ID   SPT4_CRYNJ              Reviewed;         113 AA.
AC   P0CR68; Q55MV6; Q5KB83;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=Transcription elongation factor SPT4;
DE   AltName: Full=Chromatin elongation factor SPT4;
GN   Name=SPT4; OrderedLocusNames=CNI03430;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: The SPT4-SPT5 complex mediates both activation and inhibition
CC       of transcription elongation, and plays a role in pre-mRNA processing.
CC       This complex seems to be important for the stability of the RNA
CC       polymerase II elongation machinery on the chromatin template but not
CC       for the inherent ability of this machinery to translocate down the gene
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the SPT4-SPT5 complex. Interacts with RNA
CC       polymerase II (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere
CC       {ECO:0000250}. Note=Centromere and heterochromatin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SPT4 family. {ECO:0000305}.
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DR   EMBL; AE017349; AAW45583.1; -; Genomic_DNA.
DR   RefSeq; XP_572890.1; XM_572890.1.
DR   AlphaFoldDB; P0CR68; -.
DR   SMR; P0CR68; -.
DR   STRING; 5207.AAW45583; -.
DR   PaxDb; P0CR68; -.
DR   EnsemblFungi; AAW45583; AAW45583; CNI03430.
DR   GeneID; 3259485; -.
DR   KEGG; cne:CNI03430; -.
DR   VEuPathDB; FungiDB:CNI03430; -.
DR   eggNOG; KOG3490; Eukaryota.
DR   HOGENOM; CLU_138052_2_0_1; -.
DR   InParanoid; P0CR68; -.
DR   OMA; FDGMIAV; -.
DR   OrthoDB; 1617752at2759; -.
DR   Proteomes; UP000002149; Chromosome 9.
DR   GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR   GO; GO:0032044; C:DSIF complex; IBA:GO_Central.
DR   GO; GO:0000993; F:RNA polymerase II complex binding; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006325; P:chromatin organization; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; IBA:GO_Central.
DR   GO; GO:0032786; P:positive regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR   GO; GO:0034243; P:regulation of transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR   CDD; cd07973; Spt4; 1.
DR   Gene3D; 3.30.40.210; -; 1.
DR   InterPro; IPR029040; RPABC4/Spt4.
DR   InterPro; IPR009287; Spt4.
DR   InterPro; IPR022800; Spt4/RpoE2_Znf.
DR   InterPro; IPR038510; Spt4_sf.
DR   PANTHER; PTHR12882; PTHR12882; 1.
DR   Pfam; PF06093; Spt4; 1.
DR   PIRSF; PIRSF025023; Spt4; 1.
DR   SMART; SM01389; Spt4; 1.
DR   SUPFAM; SSF63393; SSF63393; 1.
PE   3: Inferred from homology;
KW   Centromere; Chromosome; Metal-binding; mRNA processing; Nucleus;
KW   Reference proteome; Transcription; Zinc; Zinc-finger.
FT   CHAIN           1..113
FT                   /note="Transcription elongation factor SPT4"
FT                   /id="PRO_0000238549"
FT   ZN_FING         13..33
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   113 AA;  12634 MW;  703B7FC4BC376729 CRC64;
     MPPKSGRAEL RACLVCSILQ STNDFLTQGC PNCEDILEMR GSAERVAECT SLLYDGMIAM
     IEPSESWVAR WQRIDKRMRG IYAVRVTGRA PQDVIDAIEA RGGVYRPRDA VED