SPT4_METJA
ID SPT4_METJA Reviewed; 59 AA.
AC Q57839;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Transcription elongation factor Spt4 {ECO:0000255|HAMAP-Rule:MF_00949};
GN Name=spt4 {ECO:0000255|HAMAP-Rule:MF_00949};
GN Synonyms=rpoE'', rpoE2 {ECO:0000303|PubMed:8688087};
GN OrderedLocusNames=MJ0396;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH ZINC, FUNCTION, AND
RP SUBUNIT.
RX PubMed=20197319; DOI=10.1093/nar/gkq135;
RA Hirtreiter A., Damsma G.E., Cheung A.C., Klose D., Grohmann D., Vojnic E.,
RA Martin A.C., Cramer P., Werner F.;
RT "Spt4/5 stimulates transcription elongation through the RNA polymerase
RT clamp coiled-coil motif.";
RL Nucleic Acids Res. 38:4040-4051(2010).
CC -!- FUNCTION: Stimulates transcription elongation. {ECO:0000255|HAMAP-
CC Rule:MF_00949, ECO:0000269|PubMed:20197319}.
CC -!- SUBUNIT: Heterodimer composed of Spt4 and Spt5. {ECO:0000255|HAMAP-
CC Rule:MF_00949, ECO:0000269|PubMed:20197319}.
CC -!- INTERACTION:
CC Q57839; Q57818: spt5; NbExp=3; IntAct=EBI-15739363, EBI-15739382;
CC -!- SIMILARITY: Belongs to the archaeal Spt4 family. {ECO:0000255|HAMAP-
CC Rule:MF_00949}.
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DR EMBL; L77117; AAB98386.1; -; Genomic_DNA.
DR PIR; D64349; D64349.
DR RefSeq; WP_010869895.1; NC_000909.1.
DR PDB; 3LPE; X-ray; 1.90 A; B/D/F/H=1-59.
DR PDB; 4ZN1; X-ray; 2.80 A; B=2-59.
DR PDB; 4ZN3; X-ray; 2.30 A; B=2-59.
DR PDBsum; 3LPE; -.
DR PDBsum; 4ZN1; -.
DR PDBsum; 4ZN3; -.
DR AlphaFoldDB; Q57839; -.
DR SMR; Q57839; -.
DR DIP; DIP-46332N; -.
DR IntAct; Q57839; 1.
DR STRING; 243232.MJ_0396; -.
DR EnsemblBacteria; AAB98386; AAB98386; MJ_0396.
DR GeneID; 1451253; -.
DR KEGG; mja:MJ_0396; -.
DR eggNOG; arCOG04077; Archaea.
DR HOGENOM; CLU_199467_0_0_2; -.
DR InParanoid; Q57839; -.
DR OMA; VCRECHR; -.
DR OrthoDB; 119723at2157; -.
DR PhylomeDB; Q57839; -.
DR EvolutionaryTrace; Q57839; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 2.20.28.90; -; 1.
DR HAMAP; MF_00949; Spt4_arch; 1.
DR InterPro; IPR029040; RPABC4/Spt4.
DR InterPro; IPR022800; Spt4/RpoE2_Znf.
DR InterPro; IPR007178; Spt4_arch.
DR InterPro; IPR038589; Spt4_dom_sf.
DR PANTHER; PTHR40704; PTHR40704; 1.
DR Pfam; PF06093; Spt4; 1.
DR SMART; SM01389; Spt4; 1.
DR SUPFAM; SSF63393; SSF63393; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Metal-binding; Reference proteome; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..59
FT /note="Transcription elongation factor Spt4"
FT /id="PRO_0000074037"
FT BINDING 4
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00949,
FT ECO:0000269|PubMed:20197319"
FT BINDING 7
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00949,
FT ECO:0000269|PubMed:20197319"
FT BINDING 16
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00949,
FT ECO:0000269|PubMed:20197319"
FT BINDING 19
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00949,
FT ECO:0000269|PubMed:20197319"
FT STRAND 2..4
FT /evidence="ECO:0007829|PDB:3LPE"
FT TURN 5..7
FT /evidence="ECO:0007829|PDB:3LPE"
FT STRAND 9..15
FT /evidence="ECO:0007829|PDB:3LPE"
FT TURN 17..19
FT /evidence="ECO:0007829|PDB:3LPE"
FT STRAND 22..25
FT /evidence="ECO:0007829|PDB:3LPE"
FT STRAND 30..34
FT /evidence="ECO:0007829|PDB:3LPE"
FT TURN 36..38
FT /evidence="ECO:0007829|PDB:3LPE"
FT HELIX 40..44
FT /evidence="ECO:0007829|PDB:3LPE"
FT STRAND 51..53
FT /evidence="ECO:0007829|PDB:3LPE"
FT STRAND 55..57
FT /evidence="ECO:0007829|PDB:3LPE"
SQ SEQUENCE 59 AA; 6578 MW; CFC635FF7C1B0919 CRC64;
MRACLKCKYL TNDEICPICH SPTSENWIGL LIVINPEKSE IAKKAGIDIK GKYALSVKE
