SPT51_ARATH
ID SPT51_ARATH Reviewed; 1041 AA.
AC Q9STN3; F4JFX7;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 2.
DT 25-MAY-2022, entry version 135.
DE RecName: Full=Putative transcription elongation factor SPT5 homolog 1;
GN OrderedLocusNames=At4g08350; ORFNames=T28D5.40;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
CC -!- FUNCTION: May regulate transcription elongation by RNA polymerase II.
CC May enhance transcriptional pausing at sites proximal to the promoter,
CC which may in turn facilitate the assembly of an elongation competent
CC RNA polymerase II complex (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SPT5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB52557.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB77960.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL109819; CAB52557.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161511; CAB77960.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE82629.1; -; Genomic_DNA.
DR PIR; T14189; T14189.
DR RefSeq; NP_192575.3; NM_116904.6.
DR AlphaFoldDB; Q9STN3; -.
DR SMR; Q9STN3; -.
DR BioGRID; 11691; 2.
DR IntAct; Q9STN3; 1.
DR MINT; Q9STN3; -.
DR STRING; 3702.AT4G08350.1; -.
DR iPTMnet; Q9STN3; -.
DR PaxDb; Q9STN3; -.
DR PRIDE; Q9STN3; -.
DR ProteomicsDB; 245202; -.
DR EnsemblPlants; AT4G08350.1; AT4G08350.1; AT4G08350.
DR GeneID; 826391; -.
DR Gramene; AT4G08350.1; AT4G08350.1; AT4G08350.
DR KEGG; ath:AT4G08350; -.
DR Araport; AT4G08350; -.
DR TAIR; locus:2137415; AT4G08350.
DR eggNOG; KOG1999; Eukaryota.
DR HOGENOM; CLU_003537_0_1_1; -.
DR InParanoid; Q9STN3; -.
DR OMA; VGYMNTP; -.
DR OrthoDB; 828863at2759; -.
DR PRO; PR:Q9STN3; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9STN3; baseline and differential.
DR Genevisible; Q9STN3; AT.
DR GO; GO:0032044; C:DSIF complex; IBA:GO_Central.
DR GO; GO:0005840; C:ribosome; IEA:InterPro.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR CDD; cd06081; KOW_Spt5_1; 1.
DR CDD; cd06082; KOW_Spt5_2; 1.
DR CDD; cd06083; KOW_Spt5_3; 1.
DR CDD; cd06084; KOW_Spt5_4; 1.
DR CDD; cd06085; KOW_Spt5_5; 1.
DR CDD; cd06086; KOW_Spt5_6; 1.
DR CDD; cd09888; NGN_Euk; 1.
DR Gene3D; 2.30.30.30; -; 4.
DR Gene3D; 3.30.70.940; -; 1.
DR InterPro; IPR005824; KOW.
DR InterPro; IPR041973; KOW_Spt5_1.
DR InterPro; IPR041975; KOW_Spt5_2.
DR InterPro; IPR041976; KOW_Spt5_3.
DR InterPro; IPR041977; KOW_Spt5_4.
DR InterPro; IPR041978; KOW_Spt5_5.
DR InterPro; IPR041980; KOW_Spt5_6.
DR InterPro; IPR005100; NGN-domain.
DR InterPro; IPR006645; NGN_dom.
DR InterPro; IPR036735; NGN_dom_sf.
DR InterPro; IPR039385; NGN_Euk.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR005825; Ribosomal_L24/26_CS.
DR InterPro; IPR039659; SPT5.
DR InterPro; IPR022581; Spt5_N.
DR InterPro; IPR017071; TF_Spt5_eukaryote.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR11125; PTHR11125; 1.
DR Pfam; PF00467; KOW; 1.
DR Pfam; PF03439; Spt5-NGN; 1.
DR Pfam; PF11942; Spt5_N; 1.
DR PIRSF; PIRSF036945; Spt5; 1.
DR SMART; SM00739; KOW; 6.
DR SMART; SM00738; NGN; 1.
DR SUPFAM; SSF50104; SSF50104; 2.
DR PROSITE; PS01108; RIBOSOMAL_L24; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..1041
FT /note="Putative transcription elongation factor SPT5
FT homolog 1"
FT /id="PRO_0000208475"
FT DOMAIN 273..300
FT /note="KOW 1"
FT DOMAIN 425..452
FT /note="KOW 2"
FT DOMAIN 477..504
FT /note="KOW 3"
FT DOMAIN 601..628
FT /note="KOW 4"
FT DOMAIN 712..739
FT /note="KOW 5"
FT DOMAIN 988..1015
FT /note="KOW 6"
FT REGION 1..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 662..713
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 768..921
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..33
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..54
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 97..117
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 118..133
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 832..846
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 59
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19245862"
SQ SEQUENCE 1041 AA; 115405 MW; 8B91E6D4E5AAEA4B CRC64;
MPRSRDEDDE LDGDYEALDL EEEEEEDEEE EEERGRGGGG SRRKRGRSNF IDDYAEEDSQ
EEDDDDEDYG SSRGGKGAAS KRKKPSASIF LDREAHQVDD EDEEEEDEAE DDFIVDNGTD
LPDERGDRRY ERRFLPRDEN DEDVEDLERR IQERFSSRHH EEYDEEATEV EQQALLPSVR
DPKLWMVKCA IGREREVAVC LMQKFIDRGA DLQIRSVVAL DHLKNFIYVE ADKEAHVKEA
IKGMRNIYAN QKILLVPIRE MTDVLSVESK AIDLSRDTWV RMKIGTYKGD LAKVVDVDNV
RQRVTVKLIP RIDLQALASK LDGREVSKKK AFVPPPRFMN IDEARELHIR VERRRDHMTG
DYFENIGGML FKDGFHYKQV SLKSITVQNV TPTFDELEKF NKPSENGEGD FGGLSTLFAN
RKKGHFMKGD AVIVIKGDLK NLKGWVEKVD EENVLIRSEV KGLPDPLAVN ERELCKYFEP
GNHVKVVSGT HEGATGMVVK VDQHVLIILS DTTKEHVRVF ADHVVESSEV TTGVTKIGDY
ELHDLVLLDN LSFGVIIRLE NEAFQVLKGV PDRPEVALVK LREIKCKLEK KINVQDRYKN
VIAVKDDVRV IEGPSKGKQG PVKHIYKGVL FIYDRHHLEH AGFICAKCTS CIVVGGSRSG
ANRNGGDSLS RYGNFKAPAP VPSSPGRFQR GRGGGYNNSG GRHGGGRGRG DDSLLGTTVK
IRLGPFKGYR GPVVEVKGNS VRVELEMKIV TVDRGAISDN VATTPFRDTS RYSMGSETPM
HPSRTPLHPY MTPMRDSGAT PIHDGMRTPM RDRAWNPYTP MSPPRDNWED GNPGSWGTSP
QYQPGSPPSR AYEAPTPGSG WASTPGGSYS DAGTPRDHGS AYANAPSPYL PSTPGQPMTP
SSASYLPGTP GGQPMTPGTG LDVMSPVIGG DAEAWFMPDI LVDIHKAGED TDVGVIRDVS
DGTCKVSLGS SGEGDTIMAL PSELEIIPPR KSDRVKIVGG QYRGSTGKLI GIDGSDGIVK
IDDNLDVKIL DLALLAKFVQ P
