SPT52_ARATH
ID SPT52_ARATH Reviewed; 989 AA.
AC O80770; F4IGX3;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Putative transcription elongation factor SPT5 homolog 2;
GN OrderedLocusNames=At2g34210; ORFNames=F13P17.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: May regulate transcription elongation by RNA polymerase II.
CC May enhance transcriptional pausing at sites proximal to the promoter,
CC which may in turn facilitate the assembly of an elongation competent
CC RNA polymerase II complex (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SPT5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC27397.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004481; AAC27397.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08936.1; -; Genomic_DNA.
DR PIR; T02309; T02309.
DR RefSeq; NP_180968.2; NM_128972.2.
DR AlphaFoldDB; O80770; -.
DR SMR; O80770; -.
DR STRING; 3702.AT2G34210.1; -.
DR PaxDb; O80770; -.
DR PRIDE; O80770; -.
DR ProteomicsDB; 228317; -.
DR EnsemblPlants; AT2G34210.1; AT2G34210.1; AT2G34210.
DR GeneID; 817982; -.
DR Gramene; AT2G34210.1; AT2G34210.1; AT2G34210.
DR KEGG; ath:AT2G34210; -.
DR Araport; AT2G34210; -.
DR TAIR; locus:2040954; AT2G34210.
DR eggNOG; KOG1999; Eukaryota.
DR HOGENOM; CLU_003537_0_1_1; -.
DR InParanoid; O80770; -.
DR OrthoDB; 828863at2759; -.
DR PRO; PR:O80770; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O80770; baseline and differential.
DR Genevisible; O80770; AT.
DR GO; GO:0032044; C:DSIF complex; IBA:GO_Central.
DR GO; GO:0005840; C:ribosome; IEA:InterPro.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR CDD; cd06081; KOW_Spt5_1; 1.
DR CDD; cd06082; KOW_Spt5_2; 1.
DR CDD; cd06083; KOW_Spt5_3; 1.
DR CDD; cd06084; KOW_Spt5_4; 1.
DR CDD; cd06086; KOW_Spt5_6; 1.
DR CDD; cd09888; NGN_Euk; 1.
DR Gene3D; 2.30.30.30; -; 4.
DR Gene3D; 3.30.70.940; -; 1.
DR InterPro; IPR005824; KOW.
DR InterPro; IPR041973; KOW_Spt5_1.
DR InterPro; IPR041975; KOW_Spt5_2.
DR InterPro; IPR041976; KOW_Spt5_3.
DR InterPro; IPR041977; KOW_Spt5_4.
DR InterPro; IPR041980; KOW_Spt5_6.
DR InterPro; IPR005100; NGN-domain.
DR InterPro; IPR006645; NGN_dom.
DR InterPro; IPR036735; NGN_dom_sf.
DR InterPro; IPR039385; NGN_Euk.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR005825; Ribosomal_L24/26_CS.
DR InterPro; IPR039659; SPT5.
DR InterPro; IPR022581; Spt5_N.
DR InterPro; IPR017071; TF_Spt5_eukaryote.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR11125; PTHR11125; 1.
DR Pfam; PF00467; KOW; 3.
DR Pfam; PF03439; Spt5-NGN; 1.
DR Pfam; PF11942; Spt5_N; 1.
DR PIRSF; PIRSF036945; Spt5; 1.
DR SMART; SM00739; KOW; 6.
DR SMART; SM00738; NGN; 1.
DR SUPFAM; SSF50104; SSF50104; 2.
DR PROSITE; PS01108; RIBOSOMAL_L24; 1.
PE 3: Inferred from homology;
KW Activator; Nucleus; Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..989
FT /note="Putative transcription elongation factor SPT5
FT homolog 2"
FT /id="PRO_0000208476"
FT DOMAIN 260..287
FT /note="KOW 1"
FT DOMAIN 412..439
FT /note="KOW 2"
FT DOMAIN 464..491
FT /note="KOW 3"
FT DOMAIN 588..615
FT /note="KOW 4"
FT DOMAIN 683..710
FT /note="KOW 5"
FT DOMAIN 936..963
FT /note="KOW 6"
FT REGION 1..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 790..852
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..25
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..57
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 65..82
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 801..852
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 989 AA; 110277 MW; A940B90557C3A11C CRC64;
MSQYSDDDYS HEDDSEMEDE DEEDEYEPRS SRKGRSGKKR GRSNSDSDGR RGSKKKSSGS
AFIDWEVEVD DDVEDDDDDV DVEDGKQQLK FGDFSLCFIV SGEADLPNED SDHRRQYYQR
GFHPHEEDVD ELEKRTLERL STKYAKDDYE LDDVNDVDQQ ALLPSVRDPK LWLVKCAIGR
EREVAVCLMQ KIVDRGSEFK IRSAIALDHL QNYVYIEADM EAHVKEAIKG MRNIYANQKI
LLVPIKEMTA VLSVESKAID LSRDSWVRMK LGIYKGDLAQ VVDVDNVRKR VTVKLIPRID
LQALANKLEG TENVKKKAFA PPPRFMNIDE ARELHIRVEH RRDPMTGDYF ENIGGMLFKD
GFLYKKVSTK SIAAQNVTPT FDELERFKRP NENGEIDFVD ESTLFANRKK GHFMKGDAVI
VIKGDLKNLK GWIEKVDEEN VLIRSEMKDL PNPIAVNGRE LCKYFEPGNF VKVVSGIHEG
GTGMIVKVDQ HMLIILSDTT KEHICVFADH VAKSAEVTKG VTKIGDYELH DLVILSDFSF
GVILKLDSEA IQILKGVPDS SEVSIVKASE IKYKIWKKIN VQDRYKNVVA VKDVVRVIEG
PSKGKQGPVV QIYKGVLFIH DRHNLEHTGF ICTRCSSCVL AGGNFKTPAL VPPSPRRFQR
ADMGYNPGAG GRHQGGRGRR GDDHLVGTYV KIRLGPFKGY SGRLVEVKDK LVRVELEAKI
VTVERKAISD MTDNVVATPQ YNMGSQTPMH PSRTPLHPCM TPMRHSGATP IHDGMRTPMR
GRAWNPYMPM SPPRDNWEDG NPGSWGTSPY EAATPGSDWG SSTPGRSSYR DAGTPINNAN
APSPMTPSST SYLPTTPGGQ AMTPGTDLDV MSLDIGGDAE TRFIPGILVN VHKAGEDRNP
GVIRDVLPDG SCVVALGHRG EGETIRATQN KVSLVCPKKN ERVKILGGKY CGSTAKVIGE
DGQDGIVKLD ESLDIKILKL TILAKLVHE
