ID   SPT5_CRYNJ              Reviewed;        1152 AA.
AC   P0CR70; Q55Z86; Q5KNK1;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Transcription elongation factor SPT5;
DE   AltName: Full=Chromatin elongation factor SPT5;
GN   Name=SPT5; OrderedLocusNames=CNA06210;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: The SPT4-SPT5 complex mediates both activation and inhibition
CC       of transcription elongation, and plays a role in pre-mRNA processing.
CC       This complex seems to be important for the stability of the RNA
CC       polymerase II elongation machinery on the chromatin template but not
CC       for the inherent ability of this machinery to translocate down the gene
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the SPT4-SPT5 complex. Interacts with RNA
CC       polymerase II (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SPT5 family. {ECO:0000305}.
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DR   EMBL; AE017341; AAW41151.1; -; Genomic_DNA.
DR   RefSeq; XP_566970.1; XM_566970.1.
DR   AlphaFoldDB; P0CR70; -.
DR   SMR; P0CR70; -.
DR   STRING; 5207.AAW41151; -.
DR   PaxDb; P0CR70; -.
DR   EnsemblFungi; AAW41151; AAW41151; CNA06210.
DR   GeneID; 3253679; -.
DR   KEGG; cne:CNA06210; -.
DR   VEuPathDB; FungiDB:CNA06210; -.
DR   eggNOG; KOG1999; Eukaryota.
DR   HOGENOM; CLU_003537_1_0_1; -.
DR   InParanoid; P0CR70; -.
DR   OMA; VGYMNTP; -.
DR   OrthoDB; 828863at2759; -.
DR   Proteomes; UP000002149; Chromosome 1.
DR   GO; GO:0032044; C:DSIF complex; IBA:GO_Central.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR   CDD; cd06081; KOW_Spt5_1; 1.
DR   CDD; cd06082; KOW_Spt5_2; 1.
DR   CDD; cd06083; KOW_Spt5_3; 1.
DR   CDD; cd06084; KOW_Spt5_4; 1.
DR   CDD; cd06085; KOW_Spt5_5; 1.
DR   CDD; cd09888; NGN_Euk; 1.
DR   Gene3D; 2.30.30.30; -; 3.
DR   Gene3D; 3.30.70.940; -; 1.
DR   InterPro; IPR005824; KOW.
DR   InterPro; IPR041973; KOW_Spt5_1.
DR   InterPro; IPR041975; KOW_Spt5_2.
DR   InterPro; IPR041976; KOW_Spt5_3.
DR   InterPro; IPR041977; KOW_Spt5_4.
DR   InterPro; IPR041978; KOW_Spt5_5.
DR   InterPro; IPR005100; NGN-domain.
DR   InterPro; IPR036735; NGN_dom_sf.
DR   InterPro; IPR039385; NGN_Euk.
DR   InterPro; IPR014722; Rib_L2_dom2.
DR   InterPro; IPR039659; SPT5.
DR   InterPro; IPR024945; Spt5_C_dom.
DR   InterPro; IPR022581; Spt5_N.
DR   InterPro; IPR017071; TF_Spt5_eukaryote.
DR   InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR   PANTHER; PTHR11125; PTHR11125; 1.
DR   Pfam; PF00467; KOW; 1.
DR   Pfam; PF03439; Spt5-NGN; 1.
DR   Pfam; PF11942; Spt5_N; 1.
DR   PIRSF; PIRSF036945; Spt5; 1.
DR   SMART; SM01104; CTD; 1.
DR   SMART; SM00739; KOW; 4.
DR   SUPFAM; SSF50104; SSF50104; 1.
PE   3: Inferred from homology;
KW   mRNA processing; Nucleus; Reference proteome; Transcription.
FT   CHAIN           1..1152
FT                   /note="Transcription elongation factor SPT5"
FT                   /id="PRO_0000238559"
FT   REGION          1..104
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          906..966
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        10..35
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        46..61
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        62..94
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1152 AA;  123917 MW;  4328D22A61D856F3 CRC64;
     MSDIEIKSSP PESPAEEQEE RRLAGRKRAR AVDPDEEDDD TQVVNNQDEE KDPKEDDGEE
     GGNAQAEGAD EGEDDEDEDE DDDDDDEDED DEDDDGERRA KRRRKQKKFR FLDVEAEVDD
     EDEEEDEDND YGDVAEFIDE APEDVGARDD HQHRRLNRVF GRNEEEDVHD IVQRLKERHA
     GAARYNGDSD AVPQRLLMPG VNDPSLWKVV VKSGREHAIC ASIFRKVFAQ QYSANPIDVI
     SVFCRDSIPG MIFIEARQSA SVSAAVNGIV GIFMSRGVNL VPIEEMAPLL KMKKKDVNLT
     PGMWVRMKRG KHAGDLAQVV DVDQITSGVV GIKFIPRIDL TPREKRKERI AIGKPGGVRP
     PARLFAYDDV RKIYGRQSVR QGAQGSYLFD NDEYVDGFCI KDVKIPAVAT EDVNPTLEEI
     SRFTGDDDST AKFDLSAIAD ANKNLSTSLL FPGDKIEVYE GEQTGLYGIV EAVSPDVIAI
     KAEGGEVHGQ TVEVPARSVR KRFDVGEHVK VLGGKHTDAS GMVVEVKGDI VTLMSDLGEQ
     EIKVFSKDLR KAADTTNLTV TKGLYDVHDM VMLDSTTAGV VTKVEGGLLR ILDQNGAAKS
     VSPEQVSIRR DNKRFAVATD SQGNDMKVGD NMKETDGEMR QGEVFNIFRS IFVFLYNREY
     TDNFGVFVAR ANSLISVTPK SAVNDLTKIN PALNQQLPYG GASLMPAPTA NLNRNRLINT
     LVVVTKGTSK GLIGVIKDVQ GENARVELKH NNKTLSVNLA SLKRKDQKTG ATFPLEMAGI
     ASAAGGYGAR PNAGQYDINP YGGATAMHPS MGGQTPALMG GRTPAARFGQ TPNPYAAGVQ
     NGKTPNPYAA GVGGKTPSAA NASGGKTPAW GASGGKTPAY GMASGGKTPA YGMQGGKTPN
     AYAMAPPGPS GGRTPAYGAY GRPEASGSRP SVMAPPSAPY SAPYSAPTPA GNGAPTPAIP
     GNPYTAPTPY GAPTPYAAPT PGMPSLSAPT PGPGAGIAPT PFGAPTPYGA QSYGASAQQQ
     RGLPWDWALD FRNVIVEIGP SYRPGSRNPL HFKRGFFDGK RFGYNDIIGE NVRAILLDDP
     SVVEEIPAEY LRPAKADSQG QVVVVIGGGP EQKGQQRTTQ YENGGSWMME LEGGDMAPLV
     VDGADLCRIW KV