SPT5_NEUCR
ID SPT5_NEUCR Reviewed; 1054 AA.
AC Q7S3C4;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 19-MAR-2014, sequence version 3.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Transcription elongation factor spt5;
DE AltName: Full=Chromatin elongation factor spt5;
DE AltName: Full=Transcription elongation factor 3;
GN Name=trf-3; Synonyms=spt5; ORFNames=NCU06929;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: The spt4-spt5 complex mediates both activation and inhibition
CC of transcription elongation, and plays a role in pre-mRNA processing.
CC This complex seems to be important for the stability of the RNA
CC polymerase II elongation machinery on the chromatin template but not
CC for the inherent ability of this machinery to translocate down the gene
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the spt4-spt5 complex. Interacts with RNA
CC polymerase II (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SPT5 family. {ECO:0000305}.
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DR EMBL; CM002242; EAA30040.3; -; Genomic_DNA.
DR RefSeq; XP_959276.3; XM_954183.3.
DR AlphaFoldDB; Q7S3C4; -.
DR SMR; Q7S3C4; -.
DR STRING; 5141.EFNCRP00000006729; -.
DR EnsemblFungi; EAA30040; EAA30040; NCU06929.
DR GeneID; 3875384; -.
DR KEGG; ncr:NCU06929; -.
DR VEuPathDB; FungiDB:NCU06929; -.
DR HOGENOM; CLU_003537_1_1_1; -.
DR InParanoid; Q7S3C4; -.
DR Proteomes; UP000001805; Chromosome 7, Linkage Group VII.
DR GO; GO:0032044; C:DSIF complex; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0060195; P:negative regulation of antisense RNA transcription; IEA:EnsemblFungi.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0034243; P:regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR CDD; cd06081; KOW_Spt5_1; 1.
DR CDD; cd06082; KOW_Spt5_2; 1.
DR CDD; cd06083; KOW_Spt5_3; 1.
DR CDD; cd06084; KOW_Spt5_4; 1.
DR CDD; cd06085; KOW_Spt5_5; 1.
DR CDD; cd09888; NGN_Euk; 1.
DR Gene3D; 2.30.30.30; -; 3.
DR Gene3D; 3.30.70.940; -; 1.
DR InterPro; IPR005824; KOW.
DR InterPro; IPR041973; KOW_Spt5_1.
DR InterPro; IPR041975; KOW_Spt5_2.
DR InterPro; IPR041976; KOW_Spt5_3.
DR InterPro; IPR041977; KOW_Spt5_4.
DR InterPro; IPR041978; KOW_Spt5_5.
DR InterPro; IPR005100; NGN-domain.
DR InterPro; IPR006645; NGN_dom.
DR InterPro; IPR036735; NGN_dom_sf.
DR InterPro; IPR039385; NGN_Euk.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR039659; SPT5.
DR InterPro; IPR024945; Spt5_C_dom.
DR InterPro; IPR022581; Spt5_N.
DR InterPro; IPR017071; TF_Spt5_eukaryote.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR11125; PTHR11125; 1.
DR Pfam; PF03439; Spt5-NGN; 1.
DR Pfam; PF11942; Spt5_N; 1.
DR PIRSF; PIRSF036945; Spt5; 1.
DR SMART; SM01104; CTD; 1.
DR SMART; SM00739; KOW; 5.
DR SMART; SM00738; NGN; 1.
DR SUPFAM; SSF50104; SSF50104; 1.
PE 3: Inferred from homology;
KW mRNA processing; Nucleus; Reference proteome; Transcription.
FT CHAIN 1..1054
FT /note="Transcription elongation factor spt5"
FT /id="PRO_0000238564"
FT REGION 1..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 387..412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 726..759
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 819..897
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 909..1054
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..35
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 71..86
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 87..118
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 141..155
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 847..869
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 932..948
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1054 AA; 114289 MW; C3441F293C56F413 CRC64;
MASNSWQFAG ADDESDDDNF NPAPADMSDE ENNDNDEETT HNHRPAGKTV RQASSPVPDE
EEEEEEHDEE EDARPAKRSR RDHHDDDEDE EQDEEENEEI DEEEEEDEEE EEDEEDEDDV
HGGNRRKRRN KDRRAAFFDI EAEVDDEDEG EDEVGDGEEI EDFIDNAHPD DIADSGRLND
DRRHRELDRR REMEASLDAE KQAEILRERY GKRAPARGYG DMAIVPRRLL LPSVEDPSIW
AVRCKEGKER EVIFSIMRRI EERMGTKNEV PITAAFERGG INSVMKGYIY VEAQRQNDIL
VALDGILNVY PRSNMHLVEI KDMPDLLRVI KTPNLEPGAW VRLKKPAKHA GDLAQVIDVT
ENGLEARVRF IPRLDYGVRD DPVFQADGKR KRPGVPGPRP PQRLFSEAEA RKRHPRYLQG
NPQTNSWNYN GDDFENGFQV KDIKIQQLEV KNVNPTLEEV TKFASGAEDG TENLDLKALA
ATLKDSAKSV AYVPGDIIEV YEGEQQGVIG KATNVQGDIV TLQVTEGDLA GRTIDVPNKG
LRKRFRIGDH VKVIGGSRFR DEVGMVVKIV DDRVTLLTDQ TNTEITVFSK DLREASDIGG
QGSLGQYSLL DLVQLDATTV GCIVKVDRES VVVLDQNGDT KQVQPSQITN KLPKRKFAVA
ADRNGSEIRL DDVVREYGGQ QRQGKIIHIH RSYIFLHTTT TNENAGVFVT RANSVTTVAA
KGGRNTATAG PDLSAMNPAL KRNPTASGKQ MPAPRTFGPD RAINQTVVIR RGGYKGLLGI
VKDTTDTHAR VELHSRGKII TIPKADLSFK DKVTGKTIDI NQRGGSRGGF GGGGRGGGEF
GSRTPMASGG SDRTPAWGSS RSTARTPAWN RDAGGSRTPA WNDGSRTVNP YDGSRTPYGG
ATAYGGIGSR TPAWHSGSRT PAPDSFGHGS KTPAWSAGSD SWGSKTPGYG ASAPTPGASG
DAWGYTPGAS GGGNSSSAYD APTPGGGLSA PTPGAALNAP TPGAYSAPTP AAVSAPTPGA
WQGGWGSGGD AVSAPTPGAL GAPTPGAPGY ADDD
