SPT5_SCHPO
ID SPT5_SCHPO Reviewed; 990 AA.
AC O13936; Q9US64;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Transcription elongation factor spt5;
DE AltName: Full=Chromatin elongation factor spt5;
GN Name=spt5; ORFNames=SPAC23C4.19;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 240-350, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=ATCC 38364 / 968;
RX PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA Hiraoka Y.;
RT "Large-scale screening of intracellular protein localization in living
RT fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL Genes Cells 5:169-190(2000).
RN [3]
RP FUNCTION, AND INTERACTION WITH SPT4.
RX PubMed=11893740; DOI=10.1074/jbc.m200015200;
RA Pei Y., Shuman S.;
RT "Interactions between fission yeast mRNA capping enzymes and elongation
RT factor Spt5.";
RL J. Biol. Chem. 277:19639-19648(2002).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: The spt4-spt5 complex mediates both activation and inhibition
CC of transcription elongation, and plays a role in pre-mRNA processing.
CC This complex seems to be important for the stability of the RNA
CC polymerase II elongation machinery on the chromatin template but not
CC for the inherent ability of this machinery to translocate down the gene
CC (By similarity). {ECO:0000250, ECO:0000269|PubMed:11893740}.
CC -!- SUBUNIT: Component of the spt4-spt5 complex. Interacts with RNA
CC polymerase II (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10759889,
CC ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the SPT5 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB16890.1; -; Genomic_DNA.
DR EMBL; AB028017; BAA87321.1; -; Genomic_DNA.
DR PIR; T38274; T38274.
DR RefSeq; NP_593191.1; NM_001018587.2.
DR PDB; 4PZ8; X-ray; 3.10 A; B=827-844.
DR PDBsum; 4PZ8; -.
DR AlphaFoldDB; O13936; -.
DR SMR; O13936; -.
DR BioGRID; 278406; 17.
DR STRING; 4896.SPAC23C4.19.1; -.
DR iPTMnet; O13936; -.
DR SwissPalm; O13936; -.
DR MaxQB; O13936; -.
DR PaxDb; O13936; -.
DR PRIDE; O13936; -.
DR EnsemblFungi; SPAC23C4.19.1; SPAC23C4.19.1:pep; SPAC23C4.19.
DR GeneID; 2541916; -.
DR KEGG; spo:SPAC23C4.19; -.
DR PomBase; SPAC23C4.19; spt5.
DR VEuPathDB; FungiDB:SPAC23C4.19; -.
DR eggNOG; KOG1999; Eukaryota.
DR HOGENOM; CLU_003537_1_1_1; -.
DR InParanoid; O13936; -.
DR OMA; VGYMNTP; -.
DR PhylomeDB; O13936; -.
DR Reactome; R-SPO-113418; Formation of the Early Elongation Complex.
DR Reactome; R-SPO-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-SPO-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-SPO-72086; mRNA Capping.
DR Reactome; R-SPO-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-SPO-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR PRO; PR:O13936; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000785; C:chromatin; IC:PomBase.
DR GO; GO:0032044; C:DSIF complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003711; F:transcription elongation regulator activity; EXP:PomBase.
DR GO; GO:0140673; P:co-transcriptional chromatin reassembly; TAS:PomBase.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0060195; P:negative regulation of antisense RNA transcription; IMP:PomBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0034243; P:regulation of transcription elongation from RNA polymerase II promoter; IMP:PomBase.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR CDD; cd06081; KOW_Spt5_1; 1.
DR CDD; cd06082; KOW_Spt5_2; 1.
DR CDD; cd06083; KOW_Spt5_3; 1.
DR CDD; cd06084; KOW_Spt5_4; 1.
DR CDD; cd06085; KOW_Spt5_5; 1.
DR CDD; cd09888; NGN_Euk; 1.
DR Gene3D; 2.30.30.30; -; 3.
DR Gene3D; 3.30.70.940; -; 1.
DR InterPro; IPR005824; KOW.
DR InterPro; IPR041973; KOW_Spt5_1.
DR InterPro; IPR041975; KOW_Spt5_2.
DR InterPro; IPR041976; KOW_Spt5_3.
DR InterPro; IPR041977; KOW_Spt5_4.
DR InterPro; IPR041978; KOW_Spt5_5.
DR InterPro; IPR005100; NGN-domain.
DR InterPro; IPR006645; NGN_dom.
DR InterPro; IPR036735; NGN_dom_sf.
DR InterPro; IPR039385; NGN_Euk.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR039659; SPT5.
DR InterPro; IPR024945; Spt5_C_dom.
DR InterPro; IPR022581; Spt5_N.
DR InterPro; IPR017071; TF_Spt5_eukaryote.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR11125; PTHR11125; 1.
DR Pfam; PF00467; KOW; 1.
DR Pfam; PF03439; Spt5-NGN; 1.
DR Pfam; PF11942; Spt5_N; 1.
DR PIRSF; PIRSF036945; Spt5; 1.
DR SMART; SM01104; CTD; 1.
DR SMART; SM00739; KOW; 5.
DR SMART; SM00738; NGN; 1.
DR SUPFAM; SSF50104; SSF50104; 1.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; Nucleus; Phosphoprotein; Reference proteome;
KW Repeat; Transcription.
FT CHAIN 1..990
FT /note="Transcription elongation factor spt5"
FT /id="PRO_0000238565"
FT DOMAIN 471..498
FT /note="KOW 1"
FT DOMAIN 520..547
FT /note="KOW 2"
FT DOMAIN 727..754
FT /note="KOW 3"
FT REPEAT 818..826
FT /note="1"
FT REPEAT 827..835
FT /note="2"
FT REPEAT 836..844
FT /note="3"
FT REPEAT 845..853
FT /note="4"
FT REPEAT 854..862
FT /note="5"
FT REPEAT 863..871
FT /note="6"
FT REPEAT 872..880
FT /note="7"
FT REPEAT 881..889
FT /note="8"
FT REPEAT 890..898
FT /note="9"
FT REPEAT 899..907
FT /note="10"
FT REPEAT 908..916
FT /note="11"
FT REGION 1..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 818..916
FT /note="11 X 9 AA tandem repeats of T-P-A-W-N-X-G-[NS]-[KR]"
FT REGION 818..862
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 946..990
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..76
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 77..96
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 97..128
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 150..172
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 36
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 990 AA; 109014 MW; 6FF52652D1C8EE89 CRC64;
MDTNSPKSID KDANSTEVDA AEQDAASVKI NSTRASPNGS DLLNDDSEAA KITTNEKQSS
PVDSHNESPN DTTINKGEDG NENEVDNVNN NDKKEDEDNV EENEEEADAN EEEEEDEEDD
EEDEEDEDES GGGRRKRARH DRRNQFLDIE AEVDEDEEEL EDEEDEIGRE DGFIEEEVGA
DYVGDDRRHR ELDRQRQELQ SVDAERLAEE YREKYGRSQT VVGDTSNVPQ RLLLPSVNDP
NIWAVRCKIG KEKDIVFTIM RKAMDLQYTS SPLEIISAFQ RDSLVGYIYV EARKQSHVLD
ALNGVLNVYT NNMILVPIKE MPDLLKVQKQ VVELLPGAYV RIRRGKYAGD LAQVDNLSEN
GLTARVRIVP RIDYSDGLKR KNSATRPQAR LFNESEAFKS NPSKFSKRGP RLFLFNNEEF
EDGFLVKDIR ISSLITEGVN PTLDEVSKFN PNNEDLDLSS LALSVKGGHA EFQPGDHVEV
YVGEQTGVSG VVENVRGSVI TMVSSDGLRL DVPSRGLRKR FRHGDYVKVI AGKYKDDTGM
VVRISKDEVT FLSDTLMTEL TVFSRDLGEA SSAQAVNSAY ELHDLVQLDV NTVACIFSVD
RDTYKVIDQN GGVRTVLASQ ITMRHSNRRG VATDRNGAEI RIGDKVKEVG GEGKQGTILH
IYRAFVFLHN RDIAENNGVF SARSRNVATI AAKGARISAD LTKMNPALSN GPALPPVANL
KRTIGRDKAI GATVRIRRGP MKGLLGVIKD TTDANARVEL HTGNKMVTIP KENLLYTTKT
GELISYTEFI ERSRGIRPGS ISTADGPNVP NWAQGARTPA VANGSRTPAW NTGSRTPAWN
SGSKTPAWNS GSRTPAWNSG NKTPAWNAGS RTPAWNSGNK TPAWNVGNKT PAWNSGAKTP
AWNAGNKTPS WNNGTKTPAW NANQTPMVAN GTNTSWGQTP AYGGFSETNW DTEDNSKPYT
APTPGAWAAP TPGGWDDEEG DSPKYVPPSP
