SPT5_USTMA
ID SPT5_USTMA Reviewed; 954 AA.
AC Q4PIC4; A0A0D1E712;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 16-SEP-2015, sequence version 2.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Transcription elongation factor SPT5;
DE AltName: Full=Chromatin elongation factor SPT5;
GN Name=SPT5; ORFNames=UMAG_10033;
OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=237631;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=17080091; DOI=10.1038/nature05248;
RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA Birren B.W.;
RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT maydis.";
RL Nature 444:97-101(2006).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=521 / FGSC 9021;
RA Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The SPT4-SPT5 complex mediates both activation and inhibition
CC of transcription elongation, and plays a role in pre-mRNA processing.
CC This complex seems to be important for the stability of the RNA
CC polymerase II elongation machinery on the chromatin template but not
CC for the inherent ability of this machinery to translocate down the gene
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the SPT4-SPT5 complex. Interacts with RNA
CC polymerase II (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SPT5 family. {ECO:0000305}.
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DR EMBL; CM003140; KIS71699.1; -; Genomic_DNA.
DR RefSeq; XP_011386622.1; XM_011388320.1.
DR AlphaFoldDB; Q4PIC4; -.
DR SMR; Q4PIC4; -.
DR STRING; 5270.UM00139P0; -.
DR EnsemblFungi; KIS71699; KIS71699; UMAG_10033.
DR GeneID; 23566108; -.
DR KEGG; uma:UMAG_10033; -.
DR VEuPathDB; FungiDB:UMAG_10033; -.
DR eggNOG; KOG1999; Eukaryota.
DR HOGENOM; CLU_003537_1_1_1; -.
DR InParanoid; Q4PIC4; -.
DR OrthoDB; 828863at2759; -.
DR Proteomes; UP000000561; Chromosome 1.
DR GO; GO:0032044; C:DSIF complex; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0060195; P:negative regulation of antisense RNA transcription; IEA:EnsemblFungi.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0034243; P:regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR CDD; cd06081; KOW_Spt5_1; 1.
DR CDD; cd06082; KOW_Spt5_2; 1.
DR CDD; cd06083; KOW_Spt5_3; 1.
DR CDD; cd06084; KOW_Spt5_4; 1.
DR CDD; cd06085; KOW_Spt5_5; 1.
DR CDD; cd09888; NGN_Euk; 1.
DR Gene3D; 2.30.30.30; -; 3.
DR Gene3D; 3.30.70.940; -; 1.
DR InterPro; IPR005824; KOW.
DR InterPro; IPR041973; KOW_Spt5_1.
DR InterPro; IPR041975; KOW_Spt5_2.
DR InterPro; IPR041976; KOW_Spt5_3.
DR InterPro; IPR041977; KOW_Spt5_4.
DR InterPro; IPR041978; KOW_Spt5_5.
DR InterPro; IPR005100; NGN-domain.
DR InterPro; IPR006645; NGN_dom.
DR InterPro; IPR036735; NGN_dom_sf.
DR InterPro; IPR039385; NGN_Euk.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR039659; SPT5.
DR InterPro; IPR022581; Spt5_N.
DR InterPro; IPR017071; TF_Spt5_eukaryote.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR11125; PTHR11125; 1.
DR Pfam; PF00467; KOW; 1.
DR Pfam; PF03439; Spt5-NGN; 1.
DR Pfam; PF11942; Spt5_N; 1.
DR PIRSF; PIRSF036945; Spt5; 1.
DR SMART; SM00739; KOW; 5.
DR SMART; SM00738; NGN; 1.
DR SUPFAM; SSF50104; SSF50104; 1.
PE 3: Inferred from homology;
KW mRNA processing; Nucleus; Reference proteome; Repeat; Transcription.
FT CHAIN 1..954
FT /note="Transcription elongation factor SPT5"
FT /id="PRO_0000238566"
FT DOMAIN 520..545
FT /note="KOW"
FT /evidence="ECO:0000255"
FT REGION 1..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 696..723
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 797..834
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 933..954
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..39
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..84
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 107..125
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 818..834
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 954 AA; 104010 MW; 290EDADF8769FA2B CRC64;
MDDNIEIKRE DQTSHALVPD KDRKSRIQHG GKRPIDPFAA EDDDEEEERG DAVDEQDQDD
DEDDDEDDDD DDDDEDDDED DEEDDTARGQ RRRAKKQRRN RFLDVEAEVD DDEEDLDEEE
EELAREDGFI EEDIPDDTED VQRRAAADNQ RLDRFRRQEE DTNAEALAEE LRQRYGRSAR
YAAQSDYAEV PQRLLMPSVE DPSLWGVPCK PGRERDIVMT LVRKAMAENF TSSPMRIISA
FCRDSIPGRV YVEARRADDV VKACNGLAGA YARQTTSLHL IPISEMADLL KLQKVQNEIQ
VGGWVRIKRG KYAGDLAQVL DVSENGEEVG LKMIPRIDLN PKDSGLYTDS LGRKRKKGAG
SSATTVAFRP PQRFFNPEEV QKAYPRDTPT KRGSQWVFQN DSYRDGYLEK DVRVTGIITE
NVNPTLDEIT KFAGESSIED GINKLGAVDL SLIADASKKA TEALIQPKDQ VEIFEGEQAG
VYGTVKAINN EVITIQLEHE DLMDQIVEVP ARSVRKRFKP GDHIKVMSGK HADETGLVVK
VEDNVTTFLS DLSMQEVSVF SKDIRQAAEV GSGVNVISGY ELHNLVQLDA QTVGIIFNIE
RESFKVLDQT GQVVTVKPHQ ISTKKDTSRA FALDHDGNEI RAGDMVKEVA GPFSRLRQGQ
VLHIYQSMVV FLHNREYTEN GGVFIARARS LEPLAPKSVS TKTKKDGGDL SKMNPALSNL
SGGGANDVRV EGVRRFGGRD MHKGKNVAII KGPYKTYRGR ITETTGNMAR IELTSVSKPI
TVSLDWLVEK DPITGRSTKL NAGGFGGPSR GGHSGTGSRM GGASSNPYSS GSTSMGAGGR
YNPYGGSQPA AAVGSYGGSS GTGAGGYGGY SQPVATSAAG ASGYGGTGGI GARTPAYNPY
AGDGGKTPAY NPYGDGGKTP AYNPLGDGGK TPAYNAAADG GRTPGYNPYA GDGW
