SPT62_ARATH
ID SPT62_ARATH Reviewed; 1322 AA.
AC Q9CAM1;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 13-FEB-2019, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Transcription elongation factor SPT6-like {ECO:0000305};
GN OrderedLocusNames=At1g63210 {ECO:0000312|Araport:AT1G63210};
GN ORFNames=F16M19.22 {ECO:0000312|EMBL:AAG51608.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP PROTEIN SEQUENCE OF 1-12.
RX PubMed=28432195; DOI=10.1074/mcp.m116.066662;
RA Willems P., Ndah E., Jonckheere V., Stael S., Sticker A., Martens L.,
RA Van Breusegem F., Gevaert K., Van Damme P.;
RT "N-terminal proteomics assisted profiling of the unexplored translation
RT initiation landscape in Arabidopsis thaliana.";
RL Mol. Cell. Proteomics 16:1064-1080(2017).
CC -!- FUNCTION: Transcription elongation factor that enhances the
CC transcription elongation by RNA polymerase II (RNAPII).
CC {ECO:0000250|UniProtKB:Q7KZ85}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the SPT6 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51608.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AEE34070.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC010795; AAG51608.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34070.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; NP_176510.1; NM_105000.1.
DR AlphaFoldDB; Q9CAM1; -.
DR SMR; Q9CAM1; -.
DR STRING; 3702.AT1G63210.1; -.
DR iPTMnet; Q9CAM1; -.
DR PaxDb; Q9CAM1; -.
DR PeptideAtlas; Q9CAM1; -.
DR PRIDE; Q9CAM1; -.
DR ProteomicsDB; 228370; -.
DR EnsemblPlants; AT1G63210.1; AT1G63210.1; AT1G63210.
DR GeneID; 842626; -.
DR Gramene; AT1G63210.1; AT1G63210.1; AT1G63210.
DR KEGG; ath:AT1G63210; -.
DR Araport; AT1G63210; -.
DR TAIR; locus:2015198; AT1G63210.
DR eggNOG; KOG1856; Eukaryota.
DR HOGENOM; CLU_001680_2_0_1; -.
DR InParanoid; Q9CAM1; -.
DR OrthoDB; 56990at2759; -.
DR PhylomeDB; Q9CAM1; -.
DR PRO; PR:Q9CAM1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAM1; baseline and differential.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0008023; C:transcription elongation factor complex; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0031491; F:nucleosome binding; IBA:GO_Central.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0042789; P:mRNA transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0034728; P:nucleosome organization; IBA:GO_Central.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR CDD; cd09928; SH2_Cterm_SPT6_like; 1.
DR CDD; cd09918; SH2_Nterm_SPT6_like; 1.
DR Gene3D; 1.10.10.2740; -; 1.
DR Gene3D; 1.10.10.650; -; 1.
DR Gene3D; 1.10.3500.10; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.140; -; 1.
DR Gene3D; 3.30.505.10; -; 2.
DR InterPro; IPR041692; HHH_9.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR010994; RuvA_2-like.
DR InterPro; IPR022967; S1_dom.
DR InterPro; IPR003029; S1_domain.
DR InterPro; IPR036860; SH2_dom_sf.
DR InterPro; IPR028083; Spt6_acidic_N_dom.
DR InterPro; IPR042066; Spt6_death-like.
DR InterPro; IPR032706; Spt6_HHH.
DR InterPro; IPR035420; Spt6_SH2.
DR InterPro; IPR035018; Spt6_SH2_C.
DR InterPro; IPR035019; Spt6_SH2_N.
DR InterPro; IPR028231; Spt6_YqgF.
DR InterPro; IPR023323; Tex-like_dom_sf.
DR InterPro; IPR023319; Tex-like_HTH_dom_sf.
DR InterPro; IPR017072; TF_Spt6.
DR InterPro; IPR006641; YqgF/RNaseH-like_dom.
DR InterPro; IPR037027; YqgF/RNaseH-like_dom_sf.
DR PANTHER; PTHR10145; PTHR10145; 2.
DR Pfam; PF14635; HHH_7; 1.
DR Pfam; PF17674; HHH_9; 1.
DR Pfam; PF14633; SH2_2; 1.
DR Pfam; PF14632; SPT6_acidic; 1.
DR Pfam; PF14639; YqgF; 1.
DR PIRSF; PIRSF036947; Spt6; 1.
DR SMART; SM00316; S1; 1.
DR SMART; SM00732; YqgFc; 1.
DR SUPFAM; SSF47781; SSF47781; 2.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR PROSITE; PS50126; S1; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..1322
FT /note="Transcription elongation factor SPT6-like"
FT /id="PRO_0000437501"
FT DOMAIN 1017..1088
FT /note="S1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00180"
FT REGION 1..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 90..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..43
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..65
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1322 AA; 153058 MW; E4272CD963E942D1 CRC64;
MNRIDEEPQI HEDPVENREE DDEDEDDQYE FDDGFLVNGS DDGEEEEEQR HCSEKKSRSR
RKKDESFTLH EDDYLLLKDN NDTFQRNKYK RLKKSSEEED KINNDDDDDD LSHFIVDEDD
HGAQGMRRKK MKYKQGTDSN ALRYANDVFG DPEELLKLRK KDLKYNEKIE RKLEDEFEPM
VLSEKYMTEK DDEIRKLDVP ERMQIFEEAI GIVSLDDMSI QEESNWIYAR LLQEQGQGCL
INKDDIVKFL EMFHVQKLEI PFIAMYRKEQ CRSLLDSSDD DHDGSDFNLD KKLETKWHKV
LWMIQDLDRK WVLLRKRKTA LFGYYTKRFE EETCMSDLNK SLFESVIKSL QAAETEREVD
DVDSKFNLHF PHCEIDEGQY KRPNRKSQYS ICSKFGIKEF ANKFGYSAEQ LGLALSLEKV
FVDELEDAKK TPEEMALNYE CAMFGDSQAV LKGARHMAAV EISCEPSIRK YVRGIFMENA
VVSTSPTPDG NVVIDSFHRF SAVKWLSEKP LRKFDGVQWL LIQKAEEEKL LQVTFKLPEN
QMNRIISECS EHYLSVGVSK YAQLWNEQRK LILEDAVHGF VLPSMEKEAR SLLTSRAKTQ
LLSEYGQVLW NKVSSGPYRR NNNTSEEEAA PRVLACCWGP GNPETTFVML DSSGELVDVL
YSGSIGLRSR DVNDQKRKKM DQDRFLKFIR DHQPDVLALA AVNLSCVRLK DEIYEAIFQM
VEELPLNYVD DRIYDFPLVY ADESLPRLYE NSRISSEQLP QQAGIVKRAV SLGRYLQNPL
AMISTLCGPG RDILSWKLHS FQDFLDPDEK YEMVEQVMVD ITNQVGIDIN LAASHEWLFS
PLQFVSGLGP RKAASLQKSL VRAGSIFVRK ELINHGIGKK VFVNAAGFLR IRRSGLACSS
SQFIDLLDDT RIHPESYGLA QELAKDVYAH DTVRGDEDDA IEMAIEHVRD EPASLRKIVL
DEYLRSKNQE NKKETYSLIM RELSCGFQDW RSLFKEVDSD EEFYMISGET KETIGEGRIV
QATVKKVTSG KAKCVLDCGL PGILLKEDYS DDGRDIVDLS NKLCEGDIVT CKVKSILKQR
YHVLLVCKES EMRKHRNLGD VDDYYHEEKR NSVLIEKEKV PKEKKQFKSR MIVHPRFQNI
TAEQATVYLS DKNIGESIVR PSSRGLNHLT LMIKISDNVY ANKEIIEGEK ENKDIVSLQR
IGKTLKIGNE TFEDLDEVMD RYVDPLVTHL MTMLNHRKFR TGTKSEIDDL LRVEKGVNPK
MVVYSFGVSH EHPGSFILSY IRSANPHHEY IGLYPKGFKF RKRMFGDLDK LAAYFKRHID
NQ
