ID   SPT6_EMENI              Reviewed;        1417 AA.
AC   Q5B7Q7; C8VHJ8;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-NOV-2011, sequence version 2.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Transcription elongation factor spt6;
DE   AltName: Full=Chromatin elongation factor spt6;
GN   Name=spt6; ORFNames=AN3423;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
CC   -!- FUNCTION: Plays a role in maintenance of chromatin structure during RNA
CC       polymerase II transcription elongation thereby repressing transcription
CC       initiation from cryptic promoters. Mediates the reassembly of
CC       nucleosomes onto the promoters of at least a selected set of genes
CC       during repression; the nucleosome reassembly is essential for
CC       transcriptional repression (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SPT6 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAA62900.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AACD01000056; EAA62900.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BN001306; CBF82740.1; -; Genomic_DNA.
DR   RefSeq; XP_661027.1; XM_655935.1.
DR   AlphaFoldDB; Q5B7Q7; -.
DR   SMR; Q5B7Q7; -.
DR   STRING; 162425.CADANIAP00009616; -.
DR   EnsemblFungi; CBF82740; CBF82740; ANIA_03423.
DR   EnsemblFungi; EAA62900; EAA62900; AN3423.2.
DR   GeneID; 2874464; -.
DR   KEGG; ani:AN3423.2; -.
DR   VEuPathDB; FungiDB:AN3423; -.
DR   eggNOG; KOG1856; Eukaryota.
DR   HOGENOM; CLU_001680_0_1_1; -.
DR   InParanoid; Q5B7Q7; -.
DR   OMA; LCNGFKT; -.
DR   OrthoDB; 56990at2759; -.
DR   Proteomes; UP000000560; Chromosome VI.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0000791; C:euchromatin; IEA:EnsemblFungi.
DR   GO; GO:0005721; C:pericentric heterochromatin; IEA:EnsemblFungi.
DR   GO; GO:0008023; C:transcription elongation factor complex; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR   GO; GO:0031491; F:nucleosome binding; IBA:GO_Central.
DR   GO; GO:0001073; F:transcription antitermination factor activity, DNA binding; IEA:EnsemblFungi.
DR   GO; GO:0000433; P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose; IEA:EnsemblFungi.
DR   GO; GO:0140673; P:co-transcriptional chromatin reassembly; IEA:EnsemblFungi.
DR   GO; GO:0042789; P:mRNA transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0006334; P:nucleosome assembly; IEA:EnsemblFungi.
DR   GO; GO:0034728; P:nucleosome organization; IBA:GO_Central.
DR   GO; GO:0016973; P:poly(A)+ mRNA export from nucleus; IEA:EnsemblFungi.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR   GO; GO:0071931; P:positive regulation of transcription involved in G1/S transition of mitotic cell cycle; IEA:EnsemblFungi.
DR   GO; GO:0000414; P:regulation of histone H3-K36 methylation; IEA:EnsemblFungi.
DR   GO; GO:0031440; P:regulation of mRNA 3'-end processing; IEA:EnsemblFungi.
DR   GO; GO:0043618; P:regulation of transcription from RNA polymerase II promoter in response to stress; IEA:EnsemblFungi.
DR   GO; GO:0001178; P:regulation of transcriptional start site selection at RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR   CDD; cd09928; SH2_Cterm_SPT6_like; 1.
DR   CDD; cd09918; SH2_Nterm_SPT6_like; 1.
DR   Gene3D; 1.10.10.2740; -; 1.
DR   Gene3D; 1.10.10.650; -; 1.
DR   Gene3D; 1.10.3500.10; -; 1.
DR   Gene3D; 3.30.420.140; -; 1.
DR   Gene3D; 3.30.505.10; -; 2.
DR   InterPro; IPR041692; HHH_9.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR010994; RuvA_2-like.
DR   InterPro; IPR036860; SH2_dom_sf.
DR   InterPro; IPR028083; Spt6_acidic_N_dom.
DR   InterPro; IPR042066; Spt6_death-like.
DR   InterPro; IPR032706; Spt6_HHH.
DR   InterPro; IPR028088; Spt6_HTH_DNA-bd_dom.
DR   InterPro; IPR035420; Spt6_SH2.
DR   InterPro; IPR035018; Spt6_SH2_C.
DR   InterPro; IPR035019; Spt6_SH2_N.
DR   InterPro; IPR028231; Spt6_YqgF.
DR   InterPro; IPR023323; Tex-like_dom_sf.
DR   InterPro; IPR023319; Tex-like_HTH_dom_sf.
DR   InterPro; IPR017072; TF_Spt6.
DR   InterPro; IPR037027; YqgF/RNaseH-like_dom_sf.
DR   PANTHER; PTHR10145; PTHR10145; 1.
DR   Pfam; PF14635; HHH_7; 1.
DR   Pfam; PF17674; HHH_9; 1.
DR   Pfam; PF14641; HTH_44; 1.
DR   Pfam; PF14633; SH2_2; 1.
DR   Pfam; PF14632; SPT6_acidic; 1.
DR   Pfam; PF14639; YqgF; 1.
DR   PIRSF; PIRSF036947; Spt6; 1.
DR   SUPFAM; SSF47781; SSF47781; 2.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF55550; SSF55550; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; SH2 domain; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1417
FT                   /note="Transcription elongation factor spt6"
FT                   /id="PRO_0000238575"
FT   DOMAIN          1108..1177
FT                   /note="S1 motif"
FT   DOMAIN          1226..1326
FT                   /note="SH2"
FT   REGION          1..109
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          122..185
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1181..1215
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        10..28
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        41..55
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        72..92
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        122..136
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        151..168
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1183..1215
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1417 AA;  163079 MW;  85B1D0D2C2847034 CRC64;
     MSARDFVEGE AMLDEEENEE ELVDDYGDGE ERLETGGNHY DSSEEDEDED DDEDAVRAVR
     EGFIVDEDEE EEERAERRRE RRKRRREERE REDEHLDEED LELIGELNPG LQYAAAADSK
     FKRLKRGHKD RDSRQPSQAI NDFFNSDEED EPAPDYGRHR RHPGDEMDDF IEEDVFSDDE
     LQREREDLEV ARPRKTIGFG ATDTTGLDEN ALEDMRAAFG DGNEYDFALA MEEEEEQQEE
     DVEKHLDLKD VFEPSQLAEK MLTEEDNQIR LIDEPERHQI ARKPYRNVVL SEDQFREEAA
     WIANLMLLKK RLEPELREPF QRSVAKVLEF LVTDDWEVPF IFQHRKDYMI HTVKVPVNGA
     SADDSSSQYT IKAEKLLNMT DLWDIFDYDL KFKALVEKRN TIQKTYDNIR SVFSVEDPIV
     EEMLPIATTM EELQDIQDYL HFQYASQIRD LTLTNGDTNG EVQRRKALTR NFFERVRNSK
     AYGLVRAFGI TADAFAQNAL KEGRRQYTED ASERPEDMAD GLVDNDFNNS SQVLKAAKGM
     FAEEIVMSPK MRKVIRQAYY MNGAVDCFRT EKGLRRIDEQ HPYYEFKYLR DQQLSDIARS
     PELFLRMLKA EEEGLIEVKV RFENFENFRK RLYPNIESDN YSELADSWNR LRREAVDLAL
     GKLERVINRS VKENIRQECE NHVAKECREA FSQRLDQAPY KPKGMILGTV PRVLALSTGT
     GIIGRAPIHW AYVEEDGRVL ENGKFTDLSL GDKDRGIADG KDLEALVELV NRRRPDVIGV
     SGMSPETRRL YKLLTEIVDA KDLRGALYTD DRDEEVSDRL EVVIVNDEVA RLYQNSDRAK
     KDHPSFAPLT HYCVGLAKYL QSPLKEYASL GRDIVSIQFK PGQQLVAQEL LLKQLETALV
     DMVNLVGVDI NEAVSDPATA NLLPYVCGLG PRKAAHLLKI VNMTGGVVNS RFSLLGVGVQ
     YPAMGVKVWN NSASFLYIDY ESADADSDPL DNTRVHPEDY DIARKMAADA LELDEEDIKA
     ETDENGPGAI VRKLFREDAQ DRVNDLILEE YAEQLEKNLN QRKRATLETI RAELQQPYEE
     LRKHFVFLST DDIFTMLTGE TAQTLAEGMV VPISIKSIRD DHIEGKLDCG VDALVGESEM
     TDRYDIPVRA IYSLHQTVPA KVMFLNRKTF TCNVSLREEQ VSRPSRPAAD RAHAGEWDYR
     QEEQDREALE AKTQDGGRTM RVIKHPLFRP FNSTQAVEFL GSQSRGDVVI RPSSKGPDHL
     AVTWKVADGI FQHIDVLELD KENEFSVGRT LKVGGRFTYS DLDDLIFNHV KAMAKKVDEM
     MLHEKYQEGS KDSTYQWLET YTKANPRRSA YAFCIDPKHA GYFFLCFKAG EHAQVHSWPV
     KVIPQGYELQ RNPYPDMRAL CNGFKLLFTN MQSGKRR