SPT8_SCHPO
ID SPT8_SCHPO Reviewed; 526 AA.
AC O60097;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 25-MAY-2022, entry version 153.
DE RecName: Full=Transcription factor spt8;
GN Name=spt8; Synonyms=sep9; ORFNames=SPBC14C8.17c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Functions as a component of the transcription regulatory
CC histone acetylation (HAT) complex SAGA. SAGA is involved in RNA
CC polymerase II-dependent transcriptional regulation of approximately 10%
CC of yeast genes. At the promoters, SAGA is required for recruitment of
CC the basal transcription machinery (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the SAGA complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the WD repeat SPT8 family. {ECO:0000305}.
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DR EMBL; CU329671; CAA18434.1; -; Genomic_DNA.
DR PIR; T39445; T39445.
DR RefSeq; NP_595920.1; NM_001021828.2.
DR AlphaFoldDB; O60097; -.
DR SMR; O60097; -.
DR BioGRID; 276326; 15.
DR IntAct; O60097; 2.
DR MINT; O60097; -.
DR STRING; 4896.SPBC14C8.17c.1; -.
DR iPTMnet; O60097; -.
DR SwissPalm; O60097; -.
DR MaxQB; O60097; -.
DR PaxDb; O60097; -.
DR EnsemblFungi; SPBC14C8.17c.1; SPBC14C8.17c.1:pep; SPBC14C8.17c.
DR GeneID; 2539775; -.
DR KEGG; spo:SPBC14C8.17c; -.
DR PomBase; SPBC14C8.17c; spt8.
DR VEuPathDB; FungiDB:SPBC14C8.17c; -.
DR eggNOG; ENOG502QS8F; Eukaryota.
DR HOGENOM; CLU_010934_1_0_1; -.
DR InParanoid; O60097; -.
DR OMA; IWDRRQP; -.
DR PhylomeDB; O60097; -.
DR PRO; PR:O60097; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0000785; C:chromatin; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0000124; C:SAGA complex; IDA:PomBase.
DR GO; GO:0017025; F:TBP-class protein binding; IBA:GO_Central.
DR GO; GO:0003713; F:transcription coactivator activity; ISO:PomBase.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0006325; P:chromatin organization; IBA:GO_Central.
DR GO; GO:0006338; P:chromatin remodeling; IC:PomBase.
DR GO; GO:0045815; P:epigenetic maintenance of chromatin in transcription-competent conformation; IC:PomBase.
DR GO; GO:1900237; P:positive regulation of induction of conjugation with cellular fusion; IMP:PomBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; EXP:PomBase.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW Transcription; Transcription regulation; WD repeat.
FT CHAIN 1..526
FT /note="Transcription factor spt8"
FT /id="PRO_0000358862"
FT REPEAT 116..155
FT /note="WD 1"
FT REPEAT 186..225
FT /note="WD 2"
FT REPEAT 230..271
FT /note="WD 3"
FT REPEAT 348..389
FT /note="WD 4"
FT REPEAT 395..434
FT /note="WD 5"
FT REPEAT 440..478
FT /note="WD 6"
FT REGION 1..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 327..364
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..75
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..97
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 327..341
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 342..364
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 526 AA; 58418 MW; 5F4096B0233B3D5C CRC64;
MEAEESESFS YPMLLPSDGL YPEDDDDADM DQDKNPEENV MNEKEDEDVD AVDEKSGEED
VEMDTMEDEN ENDEDTEQTS EKKETEETPK ENPLEKLKQK ASTASFYDVV PTLAIPMATS
INAFAFTSDL KWLFTGGEDG YIRKYDFFPS INGDLSLTVA QRHPFVDTVT KAGILLNYWE
NAYDGNKPSS VYSLAAHSRG LWVLSGVNNG DIILYSTRHQ EGYPVTSLKK HTAPVSCLAL
HGSEKKVLSG SWDKMVYYWD LNTGDAISTY NCESGQISNI VYRPSGAVNP WGSESDDMRS
LFGTPASSSS YDALFDEEVE KAIEEETKSV QANEENETEK PSQTESTTNN DNIKAPSESS
SEESNIFLTT SIDGVMNVWD HRMVDSVLKY PVPKGVPPWA MSACWSPDGN NIYIGRRNGI
VEEYNIHSGK EPVRSLKMPL DSGPVSNVYS MPNGRHLVIS SFDNIRLYDL QSKAGIGFLI
IPGHHGGLVS SLYVDTSCQF MFSASGNRGW QGTTTEVFLG YQIMTS
