SPTA3_MOUSE
ID SPTA3_MOUSE Reviewed; 193 AA.
AC Q9D9T6; Q80ZS4; Q9D5L5; Q9DAF5;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Spermatogenesis-associated protein 3;
DE AltName: Full=Testis and spermatogenesis cell-related protein 1;
DE Short=mTSARG1;
DE AltName: Full=Testis spermatocyte apoptosis-related protein 1;
GN Name=Spata3; Synonyms=Tsarg1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=12812072;
RA Fu J.J., Lu G.X., Li L.Y., Liu G., Xing X.W., Liu S.F.;
RT "Molecular cloning for testis spermatogenesis cell apoptosis related gene
RT TSARG1 and Mtsarg1 and expression analysis for Mtsarg1 gene.";
RL Yi Chuan Xue Bao 30:25-29(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q9D9T6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9D9T6-2; Sequence=VSP_008525;
CC Name=3;
CC IsoId=Q9D9T6-3; Sequence=VSP_008525, VSP_008526, VSP_008527;
CC Name=4;
CC IsoId=Q9D9T6-4; Sequence=VSP_008523, VSP_008524;
CC -!- TISSUE SPECIFICITY: Strongly expressed in testis. Faintly expressed in
CC epididymis, ovary, spleen, kidney, lung, heart, brain, epididymis,
CC liver and skeletal muscle. {ECO:0000269|PubMed:12812072}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to competing acceptor splice
CC site. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 3]: May be due to competing acceptor splice
CC site and by intron retention. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 4]: May be due to competing donor splice site.
CC {ECO:0000305}.
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DR EMBL; AF399971; AAM90619.1; -; mRNA.
DR EMBL; AK005880; BAB24295.1; -; mRNA.
DR EMBL; AK006491; BAB24616.1; -; mRNA.
DR EMBL; AK015193; BAB29741.1; -; mRNA.
DR EMBL; BC048516; AAH48516.1; -; mRNA.
DR CCDS; CCDS15116.1; -. [Q9D9T6-2]
DR CCDS; CCDS35643.1; -. [Q9D9T6-1]
DR CCDS; CCDS48301.1; -. [Q9D9T6-3]
DR RefSeq; NP_001116204.1; NM_001122732.1. [Q9D9T6-2]
DR RefSeq; NP_081305.1; NM_027029.2. [Q9D9T6-3]
DR RefSeq; NP_081576.2; NM_027300.3. [Q9D9T6-1]
DR RefSeq; NP_082923.1; NM_028647.4. [Q9D9T6-2]
DR AlphaFoldDB; Q9D9T6; -.
DR BioGRID; 213843; 1.
DR STRING; 10090.ENSMUSP00000050509; -.
DR iPTMnet; Q9D9T6; -.
DR PhosphoSitePlus; Q9D9T6; -.
DR PaxDb; Q9D9T6; -.
DR PRIDE; Q9D9T6; -.
DR ProteomicsDB; 261637; -. [Q9D9T6-1]
DR ProteomicsDB; 261638; -. [Q9D9T6-2]
DR ProteomicsDB; 261639; -. [Q9D9T6-3]
DR Antibodypedia; 11837; 50 antibodies from 21 providers.
DR Ensembl; ENSMUST00000052854; ENSMUSP00000050509; ENSMUSG00000026226. [Q9D9T6-1]
DR Ensembl; ENSMUST00000113344; ENSMUSP00000108971; ENSMUSG00000026226. [Q9D9T6-2]
DR Ensembl; ENSMUST00000125083; ENSMUSP00000120768; ENSMUSG00000026226. [Q9D9T6-4]
DR Ensembl; ENSMUST00000130504; ENSMUSP00000116903; ENSMUSG00000026226. [Q9D9T6-2]
DR Ensembl; ENSMUST00000152501; ENSMUSP00000123194; ENSMUSG00000026226. [Q9D9T6-3]
DR Ensembl; ENSMUST00000159876; ENSMUSP00000124671; ENSMUSG00000026226. [Q9D9T6-1]
DR GeneID; 70060; -.
DR KEGG; mmu:70060; -.
DR UCSC; uc007bur.2; mouse. [Q9D9T6-3]
DR UCSC; uc007buv.2; mouse. [Q9D9T6-1]
DR CTD; 130560; -.
DR MGI; MGI:1917310; Spata3.
DR VEuPathDB; HostDB:ENSMUSG00000026226; -.
DR eggNOG; ENOG502TDUQ; Eukaryota.
DR GeneTree; ENSGT00390000003032; -.
DR HOGENOM; CLU_099077_0_0_1; -.
DR InParanoid; Q9D9T6; -.
DR OMA; PRDCGCG; -.
DR OrthoDB; 1608431at2759; -.
DR TreeFam; TF338040; -.
DR BioGRID-ORCS; 70060; 3 hits in 72 CRISPR screens.
DR PRO; PR:Q9D9T6; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q9D9T6; protein.
DR Bgee; ENSMUSG00000026226; Expressed in seminiferous tubule of testis and 35 other tissues.
DR ExpressionAtlas; Q9D9T6; baseline and differential.
DR Genevisible; Q9D9T6; MM.
DR InterPro; IPR026717; SPATA3.
DR PANTHER; PTHR22234; PTHR22234; 1.
DR Pfam; PF15662; SPATA3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome.
FT CHAIN 1..193
FT /note="Spermatogenesis-associated protein 3"
FT /id="PRO_0000072175"
FT REGION 1..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..33
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..51
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..91
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 87..89
FT /note="VPM -> QCL (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_008523"
FT VAR_SEQ 90..193
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_008524"
FT VAR_SEQ 101
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:12812072,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_008525"
FT VAR_SEQ 160..171
FT /note="KPSRKYCAPRNS -> FRMNGRAKKPWV (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_008526"
FT VAR_SEQ 172..193
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_008527"
FT CONFLICT 16
FT /note="N -> D (in Ref. 2; BAB24616)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 193 AA; 20947 MW; 6B3495A59F2D5944 CRC64;
MKKVKKKKSD SRRRRNSISP QTSSDSSQQP SSETPPSCPE PASPPSKPQP CQESTTPHQV
NPEPKPQQHT PQPLPPPEKP ASSPFLVPME PKPILPSRKA AVPLTYVAPR SCSCAACPGS
SACWHRLGLC HSRIFDVLLP RDWSSMPGRG VPNLLTFYRK PSRKYCAPRN SRASSSRNCC
CGSGGLGSCL LHG
