ID   SPUD_PSEAB              Reviewed;         367 AA.
AC   Q02UB7;
DT   07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Putrescine-binding periplasmic protein SpuD;
DE   Flags: Precursor;
GN   Name=spuD; OrderedLocusNames=PA14_03920;
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA   Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   STRAIN=UCBPP-PA14;
RX   PubMed=24965220; DOI=10.1002/pmic.201400190;
RA   Ouidir T., Jarnier F., Cosette P., Jouenne T., Hardouin J.;
RT   "Extracellular Ser/Thr/Tyr phosphorylated proteins of Pseudomonas
RT   aeruginosa PA14 strain.";
RL   Proteomics 14:2017-2030(2014).
CC   -!- FUNCTION: Putrescine-binding protein probably required for putrescine
CC       uptake into cells. Binds putrescine with high affinity, spermidine with
CC       relatively low affinity. Does not bind cadaverine or spermine.
CC       Putrescine binding induces large inter-domain conformational changes.
CC       {ECO:0000250|UniProtKB:Q9I6J1}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000255|PIRNR:PIRNR019574}.
CC       Secreted {ECO:0000269|PubMed:24965220}. Note=Could be present
CC       extracellularly due to cell outer membrane leakage. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein PotD/PotF
CC       family. {ECO:0000255|PIRNR:PIRNR019574}.
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DR   EMBL; CP000438; ABJ15262.1; -; Genomic_DNA.
DR   RefSeq; WP_003084299.1; NZ_CP034244.1.
DR   AlphaFoldDB; Q02UB7; -.
DR   SMR; Q02UB7; -.
DR   PRIDE; Q02UB7; -.
DR   EnsemblBacteria; ABJ15262; ABJ15262; PA14_03920.
DR   KEGG; pau:PA14_03920; -.
DR   HOGENOM; CLU_026974_1_4_6; -.
DR   OMA; CISIGWS; -.
DR   BioCyc; PAER208963:G1G74-328-MON; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0019808; F:polyamine binding; IEA:InterPro.
DR   GO; GO:0015846; P:polyamine transport; IEA:InterPro.
DR   InterPro; IPR006059; SBP.
DR   InterPro; IPR001188; Sperm_putr-bd.
DR   Pfam; PF13416; SBP_bac_8; 1.
DR   PIRSF; PIRSF019574; Periplasmic_polyamine_BP; 1.
DR   PRINTS; PR00909; SPERMDNBNDNG.
PE   1: Evidence at protein level;
KW   Disulfide bond; Periplasm; Secreted; Signal; Transport.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..367
FT                   /note="Putrescine-binding periplasmic protein SpuD"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000431395"
FT   BINDING         36..37
FT                   /ligand="putrescine"
FT                   /ligand_id="ChEBI:CHEBI:326268"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I6J1"
FT   BINDING         244
FT                   /ligand="putrescine"
FT                   /ligand_id="ChEBI:CHEBI:326268"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I6J1"
FT   BINDING         275
FT                   /ligand="putrescine"
FT                   /ligand_id="ChEBI:CHEBI:326268"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I6J1"
FT   DISULFID        173..236
FT                   /evidence="ECO:0000250|UniProtKB:Q9I6J1"
SQ   SEQUENCE   367 AA;  40630 MW;  8734EE5F8F9BAF94 CRC64;
     MMKRFGKTLL ALTLAGSVAG MAQAADNKVL HVYNWSDYIA PDTLEKFTKE TGIKVVYDVY
     DSNEVLEAKL LAGKSGYDVV VPSNSFLAKQ IKAGVYQKLD KSKLPNWKNL NKDLMHTLEV
     SDPGNEHAIP YMWGTIGIGY NPDKVKAAFG DNAPVDSWDL VFKPENIQKL KQCGVSFLDS
     PTEILPAALH YLGYKPDTDN PKELKAAEEL FLKIRPYVTY FHSSKYISDL ANGNICVAIG
     YSGDIYQAKS RAEEAKNKVT VKYNIPKEGA GSFFDMVAIP KDAENTEGAL AFVNFLMKPE
     IMAEITDVVQ FPNGNAAATP LVSEAIRNDP GIYPSEEVMK KLYTFPDLPA KTQRAMTRSW
     TKIKSGK