ID   SPVC_SALEN              Reviewed;         241 AA.
AC   P0A2N1; P21456;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=MAPK phosphothreonine lyase;
DE            EC=4.2.3.-;
DE   AltName: Full=27.5 kDa virulence protein;
DE   AltName: Full=Secreted effector protein SpvC;
GN   Name=spvC; Synonyms=mkaD, vsdD;
OS   Salmonella enteritidis.
OG   Plasmid pNL2001.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=149539;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AL1190;
RX   PubMed=8081495; DOI=10.1099/00221287-140-6-1307;
RA   Suzuki S., Komase K., Matsui H., Abe A., Kawahara K., Tamura Y., Kijima M.,
RA   Danbara H., Nakamura M., Sato S.;
RT   "Virulence region of plasmid pNL2001 of Salmonella enteritidis.";
RL   Microbiology 140:1307-1318(1994).
CC   -!- FUNCTION: Secreted effector that irreversibly inactivates host MAP
CC       kinases by catalyzing the dephosphorylation of the phosphothreonine
CC       residue in the pT-X-pY motif present in MAPKs, via a beta-elimination
CC       reaction leading to a dehydrobutyrine residue. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the phosphothreonine lyase family.
CC       {ECO:0000305}.
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DR   EMBL; D14490; BAA03384.1; -; Genomic_DNA.
DR   RefSeq; WP_001122242.1; NZ_WIAP01000011.1.
DR   AlphaFoldDB; P0A2N1; -.
DR   SMR; P0A2N1; -.
DR   IntAct; P0A2N1; 1.
DR   MINT; P0A2N1; -.
DR   EvolutionaryTrace; P0A2N1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   DisProt; DP02929; -.
DR   Gene3D; 3.30.2430.10; -; 1.
DR   InterPro; IPR003519; OspF/SpvC.
DR   InterPro; IPR038498; OspF/SpvC_sf.
DR   Pfam; PF03536; VRP3; 1.
DR   PRINTS; PR01342; SALVRPPROT.
PE   3: Inferred from homology;
KW   Lyase; Plasmid; Secreted; Virulence.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..241
FT                   /note="MAPK phosphothreonine lyase"
FT                   /id="PRO_0000221670"
FT   ACT_SITE        106
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        136
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   241 AA;  27646 MW;  77625271D78F8814 CRC64;
     MPINRPNLNL NIPPLNIVAA YDGAEIPSTN KHLKNNFNSL HNQMRKMPVS HFKEALDVPD
     YSGMRQSGFF AMSQGFQLNN HGYDVFIHAR RESPQSQGKF AGDKFHISVL RDMVPQAFQA
     LSGLLFSEDS PVDKWKVTDM EKVVQQARVS LGAQFTLYIK PDQENSQYSA SFLHKTRQFI
     ECLESRLSEN GVISGQCPES DVHPENWKYL SYRNELRSGR DGGEMQRQAL REEPFYRLMT
     E