ID   SPVC_SALTM              Reviewed;         241 AA.
AC   P24398;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2013, sequence version 3.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=MAPK phosphothreonine lyase;
DE            EC=4.2.3.-;
DE   AltName: Full=28 kDa virulence-associated polypeptide;
DE   AltName: Full=Mouse killing factor;
DE   AltName: Full=Secreted effector protein SpvC;
DE   AltName: Full=Virulence protein MkfA;
GN   Name=spvC; Synonyms=mkfA;
OS   Salmonella typhimurium.
OG   Plasmid pIP1350.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=90371;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=C5;
RX   PubMed=2559436; DOI=10.1016/0923-2508(89)90081-8;
RA   Norel F., Pisano M.R., Nicoli J., Popoff M.Y.;
RT   "Nucleotide sequence of the plasmid-borne virulence gene mkfA encoding a 28
RT   kDa polypeptide from Salmonella typhimurium.";
RL   Res. Microbiol. 140:263-265(1989).
CC   -!- FUNCTION: Secreted effector that irreversibly inactivates host MAP
CC       kinases by catalyzing the dephosphorylation of the phosphothreonine
CC       residue in the pT-X-pY motif present in MAPKs, via a beta-elimination
CC       reaction leading to a dehydrobutyrine residue. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the phosphothreonine lyase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X16098; CAA34225.1; -; Genomic_DNA.
DR   PIR; A43984; A43984.
DR   RefSeq; WP_001122242.1; NZ_WXYU01000026.1.
DR   RefSeq; YP_003264391.1; NC_013437.1.
DR   RefSeq; YP_003864188.1; NC_014476.2.
DR   RefSeq; YP_006955267.1; NC_019108.1.
DR   RefSeq; YP_006955407.1; NC_019109.1.
DR   RefSeq; YP_006955574.1; NC_019001.1.
DR   AlphaFoldDB; P24398; -.
DR   SMR; P24398; -.
DR   OMA; RVDQQSR; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.2430.10; -; 1.
DR   InterPro; IPR003519; OspF/SpvC.
DR   InterPro; IPR038498; OspF/SpvC_sf.
DR   Pfam; PF03536; VRP3; 1.
DR   PRINTS; PR01342; SALVRPPROT.
PE   3: Inferred from homology;
KW   Lyase; Plasmid; Secreted; Virulence.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..241
FT                   /note="MAPK phosphothreonine lyase"
FT                   /id="PRO_0000221671"
FT   ACT_SITE        106
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        136
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   241 AA;  27646 MW;  77625271D78F8814 CRC64;
     MPINRPNLNL NIPPLNIVAA YDGAEIPSTN KHLKNNFNSL HNQMRKMPVS HFKEALDVPD
     YSGMRQSGFF AMSQGFQLNN HGYDVFIHAR RESPQSQGKF AGDKFHISVL RDMVPQAFQA
     LSGLLFSEDS PVDKWKVTDM EKVVQQARVS LGAQFTLYIK PDQENSQYSA SFLHKTRQFI
     ECLESRLSEN GVISGQCPES DVHPENWKYL SYRNELRSGR DGGEMQRQAL REEPFYRLMT
     E