ID   SPVR_SALDU              Reviewed;         297 AA.
AC   P24417;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Virulence genes transcriptional activator SpvR;
GN   Name=spvR; Synonyms=vsdA;
OS   Salmonella dublin.
OG   Plasmid pSDL2.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=98360;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Lane;
RX   PubMed=2041471; DOI=10.1111/j.1365-2958.1991.tb02111.x;
RA   Krause M., Roudier C., Fierer J., Harwood J., Guiney D.;
RT   "Molecular analysis of the virulence locus of the Salmonella dublin plasmid
RT   pSDL2.";
RL   Mol. Microbiol. 5:307-316(1991).
RN   [2]
RP   DISRUPTION PHENOTYPE UPON MOUSE INFECTION.
RC   STRAIN=Lane;
RX   PubMed=1400193; DOI=10.1128/jb.174.20.6418-6423.1992;
RA   Roudier C., Fierer J., Guiney D.G.;
RT   "Characterization of translation termination mutations in the spv operon of
RT   the Salmonella virulence plasmid pSDL2.";
RL   J. Bacteriol. 174:6418-6423(1992).
RN   [3]
RP   DISRUPTION PHENOTYPE UPON INFECTION OF HUMAN MACROPHAGES.
RC   STRAIN=Lane;
RX   PubMed=12438395; DOI=10.1128/iai.70.12.7126-7135.2002;
RA   Browne S.H., Lesnick M.L., Guiney D.G.;
RT   "Genetic requirements for Salmonella-induced cytopathology in human
RT   monocyte-derived macrophages.";
RL   Infect. Immun. 70:7126-7135(2002).
CC   -!- FUNCTION: Positive regulator for the plasmid-encoded virulence factors
CC       SpvA, SpvB, and SpvC.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DISRUPTION PHENOTYPE: A disruption of this gene is no longer virulent
CC       in mouse infection. 20-fold reduction in cytopathic effects in human
CC       monocyte-derived macrophages, loss of actin depolymerization in
CC       macrophages. {ECO:0000269|PubMed:12438395, ECO:0000269|PubMed:1400193}.
CC   -!- MISCELLANEOUS: In Salmonella spp. the spv gene cluster is encoded on a
CC       highly transmissible plasmid.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; X56727; CAA40047.1; -; Genomic_DNA.
DR   RefSeq; WP_000346692.1; NZ_VDCP01000013.1.
DR   RefSeq; YP_001716110.1; NC_010422.1.
DR   AlphaFoldDB; P24417; -.
DR   SMR; P24417; -.
DR   PHI-base; PHI:4515; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW   Transcription regulation; Virulence.
FT   CHAIN           1..297
FT                   /note="Virulence genes transcriptional activator SpvR"
FT                   /id="PRO_0000105681"
FT   DOMAIN          1..61
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        21..40
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   297 AA;  33850 MW;  75779F86722DB0A3 CRC64;
     MDFLINKKLK IFITLMETGS FSIATSVLYI TRTPLSRVIS GLERELKQRL FIRKNGTLIP
     TEFAQTIYRK VKSHYIFLHA LEQEIGPTGK TKQLEIIFDE IYPESLKNLI ISALTISGQK
     TNIMGRAVNS QIIEELCQTN NCIVISARNY FHRESLVCRT SVEGGVMLFI PKKFFLCGKP
     DINRLAGTPV LFHEGAKNFN LDTIYHFFEQ TLGITNPAFS FDNVDLFSSL YRLQQGLAML
     LIPVRVCRAL GLSTDHALHI KGVALCTSLY YPTKKRETPD YRKAIKLIQQ ELKQSTF