ID   SPX2_LACLA              Reviewed;         132 AA.
AC   P60375; Q9ETA4;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-FEB-2004, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Global transcriptional regulator Spx 2 {ECO:0000255|HAMAP-Rule:MF_01132};
GN   Name=spx2 {ECO:0000255|HAMAP-Rule:MF_01132}; Synonyms=spxA2, trmA;
GN   OrderedLocusNames=LL0675; ORFNames=L73853;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
RN   [2]
RP   INVOLVEMENT IN STRESS RESPONSE.
RX   PubMed=10048028; DOI=10.1046/j.1365-2958.1999.01222.x;
RA   Duwat P., Ehrlich S.D., Gruss A.;
RT   "Effects of metabolic flux on stress response pathways in Lactococcus
RT   lactis.";
RL   Mol. Microbiol. 31:845-858(1999).
RN   [3]
RP   INVOLVEMENT IN STRESS TOLERANCE.
RX   PubMed=11454203; DOI=10.1046/j.1365-2958.2001.02503.x;
RA   Frees D., Varmanen P., Ingmer H.;
RT   "Inactivation of a gene that is highly conserved in Gram-positive bacteria
RT   stimulates degradation of non-native proteins and concomitantly increases
RT   stress tolerance in Lactococcus lactis.";
RL   Mol. Microbiol. 41:93-103(2001).
CC   -!- FUNCTION: Global transcriptional regulator that plays a key role in
CC       stress response and exerts either positive or negative regulation of
CC       genes. Acts by interacting with the C-terminal domain of the alpha
CC       subunit of the RNA polymerase (RNAP). This interaction can enhance
CC       binding of RNAP to the promoter region of target genes and stimulate
CC       their transcription, or block interaction of RNAP with activator.
CC       {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SUBUNIT: Interacts with the C-terminal domain of the alpha subunit of
CC       the RNAP. {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SIMILARITY: Belongs to the ArsC family. Spx subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01132, ECO:0000305}.
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DR   EMBL; AE005176; AAK04773.1; -; Genomic_DNA.
DR   PIR; C86709; C86709.
DR   RefSeq; NP_266831.1; NC_002662.1.
DR   RefSeq; WP_003129597.1; NC_002662.1.
DR   AlphaFoldDB; P60375; -.
DR   SMR; P60375; -.
DR   STRING; 272623.L73853; -.
DR   PaxDb; P60375; -.
DR   EnsemblBacteria; AAK04773; AAK04773; L73853.
DR   GeneID; 60355107; -.
DR   GeneID; 61108935; -.
DR   GeneID; 66441573; -.
DR   KEGG; lla:L73853; -.
DR   PATRIC; fig|272623.7.peg.722; -.
DR   eggNOG; COG1393; Bacteria.
DR   HOGENOM; CLU_116644_1_1_9; -.
DR   OMA; NGHKLPY; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   HAMAP; MF_01132; Spx; 1.
DR   InterPro; IPR006660; Arsenate_reductase-like.
DR   InterPro; IPR023731; Spx.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   InterPro; IPR006504; Tscrpt_reg_Spx/MgsR.
DR   PANTHER; PTHR30041; PTHR30041; 1.
DR   Pfam; PF03960; ArsC; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   TIGRFAMs; TIGR01617; arsC_related; 1.
DR   PROSITE; PS51353; ARSC; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Disulfide bond; Redox-active center; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..132
FT                   /note="Global transcriptional regulator Spx 2"
FT                   /id="PRO_0000162554"
FT   DISULFID        10..13
FT                   /note="Redox-active"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01132"
SQ   SEQUENCE   132 AA;  15030 MW;  F63EE4B8204136B3 CRC64;
     MITIYTAPSC TSCKKAKTWL SYHHIPFNER NLIADPLSTT EISQILQKCD DGVEGLISSR
     NRFVKTLGVD FEDISLSQAI KIISENPQIM RRPIIMDEKR LHVGYNEEEI RAFLPRTVRV
     LENGGARLRS AI