ID   SPX2_OCEIH              Reviewed;         135 AA.
AC   Q8ERE2;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 115.
DE   RecName: Full=Global transcriptional regulator Spx 2 {ECO:0000255|HAMAP-Rule:MF_01132};
GN   Name=spx2 {ECO:0000255|HAMAP-Rule:MF_01132}; OrderedLocusNames=OB1363;
OS   Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC
OS   3954 / HTE831).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus.
OX   NCBI_TaxID=221109;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831;
RX   PubMed=12235376; DOI=10.1093/nar/gkf526;
RA   Takami H., Takaki Y., Uchiyama I.;
RT   "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge
RT   and its unexpected adaptive capabilities to extreme environments.";
RL   Nucleic Acids Res. 30:3927-3935(2002).
CC   -!- FUNCTION: Global transcriptional regulator that plays a key role in
CC       stress response and exerts either positive or negative regulation of
CC       genes. Acts by interacting with the C-terminal domain of the alpha
CC       subunit of the RNA polymerase (RNAP). This interaction can enhance
CC       binding of RNAP to the promoter region of target genes and stimulate
CC       their transcription, or block interaction of RNAP with activator.
CC       {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SUBUNIT: Interacts with the C-terminal domain of the alpha subunit of
CC       the RNAP. {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SIMILARITY: Belongs to the ArsC family. Spx subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- CAUTION: Thr-13 is present instead of the conserved Cys which is
CC       potentially a redox-active site. {ECO:0000305}.
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DR   EMBL; BA000028; BAC13319.1; -; Genomic_DNA.
DR   RefSeq; WP_011065769.1; NC_004193.1.
DR   AlphaFoldDB; Q8ERE2; -.
DR   SMR; Q8ERE2; -.
DR   STRING; 221109.22777045; -.
DR   EnsemblBacteria; BAC13319; BAC13319; BAC13319.
DR   KEGG; oih:OB1363; -.
DR   eggNOG; COG1393; Bacteria.
DR   HOGENOM; CLU_116644_1_1_9; -.
DR   OrthoDB; 1606021at2; -.
DR   PhylomeDB; Q8ERE2; -.
DR   Proteomes; UP000000822; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   HAMAP; MF_01132; Spx; 1.
DR   InterPro; IPR006660; Arsenate_reductase-like.
DR   InterPro; IPR023731; Spx.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   InterPro; IPR006504; Tscrpt_reg_Spx/MgsR.
DR   PANTHER; PTHR30041; PTHR30041; 1.
DR   Pfam; PF03960; ArsC; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   TIGRFAMs; TIGR01617; arsC_related; 1.
DR   PROSITE; PS51353; ARSC; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..135
FT                   /note="Global transcriptional regulator Spx 2"
FT                   /id="PRO_0000162561"
FT   ACT_SITE        10
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01132"
SQ   SEQUENCE   135 AA;  16090 MW;  15FEACFD04EF81AF CRC64;
     MTVNIYGASC SSTRKARQWF KKHGIAYKER NILRQPLTIN ELQEILRMTV EGTDEIISTR
     SKIFKELNLN LDELPLQKLL ELIHEHPRLL KSPILMDEKR FQVGYHEDDI RQFLPRKTRE
     HLWLQWKLDL GLVKG