SPX2_ORYSJ
ID SPX2_ORYSJ Reviewed; 280 AA.
AC Q6Z784; A0A0P0VG28; A3A498;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=SPX domain-containing protein 2;
DE AltName: Full=Protein SPX DOMAIN GENE 2;
DE Short=OsSPX2;
GN Name=SPX2; OrderedLocusNames=Os02g0202200, LOC_Os02g10780;
GN ORFNames=OsJ_05799, P0419A09.34;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP TISSUE SPECIFICITY, INDUCTION BY PHOSPHATE, AND SUBCELLULAR LOCATION.
RX PubMed=19566645; DOI=10.1111/j.1744-7909.2009.00834.x;
RA Wang Z., Hu H., Huang H., Duan K., Wu Z., Wu P.;
RT "Regulation of OsSPX1 and OsSPX3 on expression of OsSPX domain genes and
RT Pi-starvation signaling in rice.";
RL J. Integr. Plant Biol. 51:663-674(2009).
RN [7]
RP GENE FAMILY, NOMENCLATURE, AND INDUCTION BY COLD.
RX PubMed=19508276; DOI=10.1111/j.1467-7652.2009.00423.x;
RA Zhao L., Liu F., Xu W., Di C., Zhou S., Xue Y., Yu J., Su Z.;
RT "Increased expression of OsSPX1 enhances cold/subfreezing tolerance in
RT tobacco and Arabidopsis thaliana.";
RL Plant Biotechnol. J. 7:550-561(2009).
RN [8]
RP FUNCTION, INTERACTION WITH PHR2, SUBCELLULAR LOCATION, INDUCTION BY PHR2,
RP AND DOMAIN.
RX PubMed=25271318; DOI=10.1073/pnas.1404680111;
RA Wang Z., Ruan W., Shi J., Zhang L., Xiang D., Yang C., Li C., Wu Z.,
RA Liu Y., Yu Y., Shou H., Mo X., Mao C., Wu P.;
RT "Rice SPX1 and SPX2 inhibit phosphate starvation responses through
RT interacting with PHR2 in a phosphate-dependent manner.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:14953-14958(2014).
CC -!- FUNCTION: Inhibits PHR2 DNA-binding activity via a Pi-dependent protein
CC interaction (PubMed:25271318). {ECO:0000269|PubMed:25271318}.
CC -!- SUBUNIT: Interacts (via SPX domain) with PHR2 (via C-terminus)
CC (PubMed:25271318). {ECO:0000269|PubMed:25271318}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19566645,
CC ECO:0000269|PubMed:25271318}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in roots, leaves and seeds.
CC {ECO:0000269|PubMed:19566645}.
CC -!- INDUCTION: Up-regulated under phosphate starvation (PubMed:19566645).
CC Up-regulated during cold stress (PubMed:19508276). Up-regulated by the
CC transcription factor PHR2 (PubMed:25271318).
CC {ECO:0000269|PubMed:19508276, ECO:0000269|PubMed:19566645,
CC ECO:0000269|PubMed:25271318}.
CC -!- DOMAIN: The SPX domain is sufficient for inhibition of PHR2 binding to
CC DNA. {ECO:0000269|PubMed:25271318}.
CC -!- MISCELLANEOUS: SPX1 and SPX2 have redundant functions in repressing the
CC activity of PHR2. {ECO:0000269|PubMed:25271318}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAZ22137.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP004869; BAD15861.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF08142.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS77525.1; -; Genomic_DNA.
DR EMBL; CM000139; EAZ22137.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK107245; BAG98008.1; -; mRNA.
DR RefSeq; XP_015626019.1; XM_015770533.1.
DR AlphaFoldDB; Q6Z784; -.
DR SMR; Q6Z784; -.
DR STRING; 4530.OS02T0202200-01; -.
DR PaxDb; Q6Z784; -.
DR PRIDE; Q6Z784; -.
DR EnsemblPlants; Os02t0202200-01; Os02t0202200-01; Os02g0202200.
DR GeneID; 4328654; -.
DR Gramene; Os02t0202200-01; Os02t0202200-01; Os02g0202200.
DR KEGG; osa:4328654; -.
DR eggNOG; KOG1161; Eukaryota.
DR HOGENOM; CLU_057600_1_1_1; -.
DR InParanoid; Q6Z784; -.
DR OMA; PTISHRN; -.
DR OrthoDB; 471106at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6Z784; OS.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0070417; P:cellular response to cold; IEP:UniProtKB.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IEP:UniProtKB.
DR InterPro; IPR004331; SPX_dom.
DR InterPro; IPR031142; SPX_prot.
DR PANTHER; PTHR45978; PTHR45978; 1.
DR Pfam; PF03105; SPX; 2.
DR PROSITE; PS51382; SPX; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..280
FT /note="SPX domain-containing protein 2"
FT /id="PRO_0000398349"
FT DOMAIN 1..162
FT /note="SPX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
FT REGION 191..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 257..280
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 197..212
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 280 AA; 30955 MW; D60DA6522E556ADD CRC64;
MKFGKSLSSQ IVEMQPEWRD NFLSYKDLKK RLNLISGGAA GERASKRRRV GGATAVTVTA
AAAGGMTLEQ AGFVGLLDAE LDKFNFFFLE KEEEYVIKQK ELRERKMASA EEVMRVRKEI
VDLHGEMVLL ENYSALNYTG LVKILKKYDK RTGSMIRLPF VQKVLQQPFF TTDLLYKLVK
ECEEMLDQLM PTNEHSVASE DGKDDSEGEE KGSKPSSSSS ANGGAVPGEA EAEDERSTDM
KSTVTAALRA LREIRSGSST VSVFSLPPLH GSNGQDEPGR
