ID   SPX3_ORYSJ              Reviewed;         277 AA.
AC   Q7XEY9; A0A0P0XU27;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=SPX domain-containing protein 3;
DE   AltName: Full=Protein SPX DOMAIN GENE 3;
DE            Short=OsSPX3;
GN   Name=SPX3; OrderedLocusNames=Os10g0392600, LOC_Os10g25310;
GN   ORFNames=OsJ_31398;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12791992; DOI=10.1126/science.1083523;
RA   Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA   Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA   Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA   Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA   Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA   Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA   Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA   Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA   Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA   Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA   Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA   Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA   Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA   Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA   Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA   Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA   Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT   "In-depth view of structure, activity, and evolution of rice chromosome
RT   10.";
RL   Science 300:1566-1569(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND INDUCTION BY
RP   PHOSPHATE.
RX   PubMed=19566645; DOI=10.1111/j.1744-7909.2009.00834.x;
RA   Wang Z., Hu H., Huang H., Duan K., Wu Z., Wu P.;
RT   "Regulation of OsSPX1 and OsSPX3 on expression of OsSPX domain genes and
RT   Pi-starvation signaling in rice.";
RL   J. Integr. Plant Biol. 51:663-674(2009).
RN   [8]
RP   GENE FAMILY, NOMENCLATURE, AND INDUCTION BY COLD.
RX   PubMed=19508276; DOI=10.1111/j.1467-7652.2009.00423.x;
RA   Zhao L., Liu F., Xu W., Di C., Zhou S., Xue Y., Yu J., Su Z.;
RT   "Increased expression of OsSPX1 enhances cold/subfreezing tolerance in
RT   tobacco and Arabidopsis thaliana.";
RL   Plant Biotechnol. J. 7:550-561(2009).
RN   [9]
RP   SUBCELLULAR LOCATION, INDUCTION BY PHOSPHATE, SUBUNIT, TISSUE SPECIFICITY,
RP   AND DISRUPTION PHENOTYPE.
RX   PubMed=24368504; DOI=10.1093/jxb/ert424;
RA   Shi J., Hu H., Zhang K., Zhang W., Yu Y., Wu Z., Wu P.;
RT   "The paralogous SPX3 and SPX5 genes redundantly modulate Pi homeostasis in
RT   rice.";
RL   J. Exp. Bot. 65:859-870(2014).
CC   -!- FUNCTION: Functional repressor of PHR2 (PubMed:24368504). Involved in
CC       maintaining cellular Pi homeostasis when plants are exposed to an
CC       external change in Pi (PubMed:24368504). Negatively regulates root-to-
CC       shoot Pi translocation redundantly with SPX3 (PubMed:24368504). Down-
CC       regulates the non-coding RNA IPS1 (At3g09922) and suppresses the
CC       responses of miR399 and PHO2 to Pi-starvation (PubMed:19566645).
CC       {ECO:0000269|PubMed:19566645, ECO:0000269|PubMed:24368504}.
CC   -!- SUBUNIT: Homo- and heterodimers with SPX5.
CC       {ECO:0000269|PubMed:24368504}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24368504}. Cytoplasm
CC       {ECO:0000269|PubMed:19566645, ECO:0000269|PubMed:24368504}.
CC   -!- TISSUE SPECIFICITY: Predominantly expressed in roots, leaves and seeds
CC       (PubMed:19566645). Expressed in root epidermis, exodermis,
CC       sclerenchymal layer, cortex and endosperm (PubMed:24368504). Under Pi
CC       starvation, also detected in mesophyll and phloem in the vascular
CC       bundles (PubMed:24368504). {ECO:0000269|PubMed:19566645,
CC       ECO:0000269|PubMed:24368504}.
CC   -!- INDUCTION: Up-regulated under phosphate starvation (PubMed:19566645,
CC       PubMed:24368504). Up-regulated during cold stress (PubMed:19508276).
CC       {ECO:0000269|PubMed:19508276, ECO:0000269|PubMed:19566645,
CC       ECO:0000269|PubMed:24368504}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype, due to the redundancy with
CC       SPX5. {ECO:0000269|PubMed:24368504}.
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DR   EMBL; DP000086; AAP53570.1; -; Genomic_DNA.
DR   EMBL; AP008216; BAF26429.1; -; Genomic_DNA.
DR   EMBL; AP014966; BAT10679.1; -; Genomic_DNA.
DR   EMBL; CM000147; EAZ15953.1; -; Genomic_DNA.
DR   EMBL; AK108539; BAG98434.1; -; mRNA.
DR   RefSeq; XP_015614909.1; XM_015759423.1.
DR   AlphaFoldDB; Q7XEY9; -.
DR   SMR; Q7XEY9; -.
DR   STRING; 4530.OS10T0392600-01; -.
DR   PaxDb; Q7XEY9; -.
DR   PRIDE; Q7XEY9; -.
DR   EnsemblPlants; Os10t0392600-01; Os10t0392600-01; Os10g0392600.
DR   GeneID; 4348535; -.
DR   Gramene; Os10t0392600-01; Os10t0392600-01; Os10g0392600.
DR   KEGG; osa:4348535; -.
DR   eggNOG; KOG1161; Eukaryota.
DR   HOGENOM; CLU_057600_1_1_1; -.
DR   InParanoid; Q7XEY9; -.
DR   OMA; CGHNDEM; -.
DR   OrthoDB; 1345784at2759; -.
DR   Proteomes; UP000000763; Chromosome 10.
DR   Proteomes; UP000007752; Chromosome 10.
DR   Proteomes; UP000059680; Chromosome 10.
DR   Genevisible; Q7XEY9; OS.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0070417; P:cellular response to cold; IEP:UniProtKB.
DR   GO; GO:0016036; P:cellular response to phosphate starvation; IEA:InterPro.
DR   InterPro; IPR004331; SPX_dom.
DR   InterPro; IPR031142; SPX_prot.
DR   PANTHER; PTHR45978; PTHR45978; 1.
DR   Pfam; PF03105; SPX; 2.
DR   PROSITE; PS51382; SPX; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..277
FT                   /note="SPX domain-containing protein 3"
FT                   /id="PRO_0000398351"
FT   DOMAIN          1..152
FT                   /note="SPX"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
SQ   SEQUENCE   277 AA;  30845 MW;  2477BED56A11F81B CRC64;
     MKFGKRLKKQ VEESLPEWRD KFLAYKRLKK LVRLVSSSSG DVGGGGGGEA EFVRLLDGEV
     DRINAFFLEQ EEEFVIRQRE LQETVEKVAG GGGGGRRPAA AEMRRVRKEI VDLHGEMVLL
     LNYSAVNYTG LAKILKKYDK RTGRLLRLPF IEKVLRQPFF TTELISRLVR DCEATMEAIF
     TSSVATTAMA GDRRTWKGCS GDAGMAPMAD QQGIFRNTVA ALATMKELRS GSSTYGRFSL
     PPMAAPASPE SDVLQSIRSD PHLKENGRIP SLQFFYA