SPX5_ORYSJ
ID SPX5_ORYSJ Reviewed; 247 AA.
AC Q7Y0F6; W8QH36;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=SPX domain-containing protein 5;
DE AltName: Full=Protein SPX DOMAIN GENE 5;
DE Short=OsSPX5;
GN Name=SPX5; OrderedLocusNames=Os03g0406100, LOC_Os03g29250;
GN ORFNames=OsJ_11210, OSJNBa0002D18.21, OSJNBb0074M06.1;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INDUCTION BY PHOSPHATE,
RP SUBUNIT, AND TISSUE SPECIFICITY.
RX PubMed=24368504; DOI=10.1093/jxb/ert424;
RA Shi J., Hu H., Zhang K., Zhang W., Yu Y., Wu Z., Wu P.;
RT "The paralogous SPX3 and SPX5 genes redundantly modulate Pi homeostasis in
RT rice.";
RL J. Exp. Bot. 65:859-870(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP TISSUE SPECIFICITY, AND INDUCTION BY PHOSPHATE; SPX1 AND SPX3.
RX PubMed=19566645; DOI=10.1111/j.1744-7909.2009.00834.x;
RA Wang Z., Hu H., Huang H., Duan K., Wu Z., Wu P.;
RT "Regulation of OsSPX1 and OsSPX3 on expression of OsSPX domain genes and
RT Pi-starvation signaling in rice.";
RL J. Integr. Plant Biol. 51:663-674(2009).
RN [8]
RP GENE FAMILY, NOMENCLATURE, AND INDUCTION BY COLD.
RX PubMed=19508276; DOI=10.1111/j.1467-7652.2009.00423.x;
RA Zhao L., Liu F., Xu W., Di C., Zhou S., Xue Y., Yu J., Su Z.;
RT "Increased expression of OsSPX1 enhances cold/subfreezing tolerance in
RT tobacco and Arabidopsis thaliana.";
RL Plant Biotechnol. J. 7:550-561(2009).
CC -!- FUNCTION: Functional repressor of PHR2 (PubMed:24368504). Involved in
CC maintaining cellular Pi homeostasis when plants are exposed to an
CC external change in Pi (PubMed:24368504). Negatively regulates root-to-
CC shoot Pi translocation redundantly with SPX3 (PubMed:24368504).
CC {ECO:0000269|PubMed:24368504}.
CC -!- SUBUNIT: Homo- and heterodimers with SPX3.
CC {ECO:0000269|PubMed:24368504}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24368504}. Cytoplasm
CC {ECO:0000269|PubMed:24368504}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in roots (PubMed:19566645).
CC Expressed in root epidermis, exodermis and the sclerenchymal layer
CC (PubMed:24368504). Under Pi starvation, also detected in mesophyll and
CC phloem in the vascular bundles (PubMed:24368504).
CC {ECO:0000269|PubMed:19566645, ECO:0000269|PubMed:24368504}.
CC -!- INDUCTION: Up-regulated under phosphate starvation (PubMed:19566645,
CC PubMed:24368504). Down-regulated by SPX1 and SPX3 (PubMed:19566645).
CC Up-regulated during cold stress (PubMed:19508276).
CC {ECO:0000269|PubMed:19508276, ECO:0000269|PubMed:19566645,
CC ECO:0000269|PubMed:24368504}.
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DR EMBL; KF267997; AHL42466.1; -; mRNA.
DR EMBL; AC133932; AAP50928.1; -; Genomic_DNA.
DR EMBL; AC134886; AAU89246.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF96518.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12246.1; -; Genomic_DNA.
DR EMBL; AP014959; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CM000140; EAZ27274.1; -; Genomic_DNA.
DR RefSeq; XP_015630420.1; XM_015774934.1.
DR AlphaFoldDB; Q7Y0F6; -.
DR SMR; Q7Y0F6; -.
DR STRING; 4530.OS03T0406100-01; -.
DR PaxDb; Q7Y0F6; -.
DR PRIDE; Q7Y0F6; -.
DR EnsemblPlants; Os03t0406100-01; Os03t0406100-01; Os03g0406100.
DR GeneID; 4333078; -.
DR Gramene; Os03t0406100-01; Os03t0406100-01; Os03g0406100.
DR KEGG; osa:4333078; -.
DR eggNOG; KOG1161; Eukaryota.
DR InParanoid; Q7Y0F6; -.
DR OrthoDB; 1345784at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0070417; P:cellular response to cold; IEP:UniProtKB.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IEP:UniProtKB.
DR InterPro; IPR004331; SPX_dom.
DR InterPro; IPR031142; SPX_prot.
DR PANTHER; PTHR45978; PTHR45978; 1.
DR Pfam; PF03105; SPX; 2.
DR PROSITE; PS51382; SPX; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..247
FT /note="SPX domain-containing protein 5"
FT /id="PRO_0000398355"
FT DOMAIN 1..139
FT /note="SPX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
FT REGION 224..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 229..247
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 247 AA; 27514 MW; AEB862975B2255CC CRC64;
MKFGKRLKRQ IEESLPEWRD HFLNYKELKR RLNAVSSPDP AAEARFLALL HAEVDKFNAF
FLEQEEDFVI RQRELQERIQ SSSSAAAEME GRVRREVVDL HGEMVLLLNY SSINYTGLAK
ILKKYDKRTG GVLRLPVIAG VLRQPFYATD LLSSLVRDCE AIMDAVFPSL PSPSAAAAAA
ARAAAEQAIF RNTVAALLTM QEVRSGSSTY GHFSLPPMTP LPDSDWLIQS VQPPPPPPPS
SPLIIPT
