SPXM3_ARATH
ID SPXM3_ARATH Reviewed; 699 AA.
AC Q93ZQ5; O82747;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=SPX domain-containing membrane protein At4g22990;
GN OrderedLocusNames=At4g22990; ORFNames=F7H19.170;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q93ZQ5-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA19814.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79254.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL031018; CAA19814.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161558; CAB79254.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84689.1; -; Genomic_DNA.
DR EMBL; AY056374; AAL08230.1; -; mRNA.
DR PIR; T05130; T05130.
DR RefSeq; NP_567674.1; NM_118428.3. [Q93ZQ5-1]
DR AlphaFoldDB; Q93ZQ5; -.
DR STRING; 3702.AT4G22990.2; -.
DR PaxDb; Q93ZQ5; -.
DR PRIDE; Q93ZQ5; -.
DR ProteomicsDB; 245249; -. [Q93ZQ5-1]
DR EnsemblPlants; AT4G22990.1; AT4G22990.1; AT4G22990. [Q93ZQ5-1]
DR GeneID; 828398; -.
DR Gramene; AT4G22990.1; AT4G22990.1; AT4G22990. [Q93ZQ5-1]
DR KEGG; ath:AT4G22990; -.
DR Araport; AT4G22990; -.
DR eggNOG; KOG1161; Eukaryota.
DR eggNOG; KOG2325; Eukaryota.
DR HOGENOM; CLU_025236_1_0_1; -.
DR InParanoid; Q93ZQ5; -.
DR PhylomeDB; Q93ZQ5; -.
DR PRO; PR:Q93ZQ5; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q93ZQ5; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:1905011; P:transmembrane phosphate ion transport from cytosol to vacuole; IBA:GO_Central.
DR CDD; cd14479; SPX-MFS_plant; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004331; SPX_dom.
DR InterPro; IPR045264; SPXM_SPX_plant.
DR Pfam; PF07690; MFS_1; 1.
DR Pfam; PF03105; SPX; 2.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS51382; SPX; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..699
FT /note="SPX domain-containing membrane protein At4g22990"
FT /id="PRO_0000398569"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 515..535
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 546..566
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 578..598
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 606..626
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 671..691
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 2..145
FT /note="SPX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
FT REGION 475..494
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 526
FT /note="E -> K (in Ref. 3; AAL08230)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 699 AA; 78449 MW; 6D566870E1F94747 CRC64;
MVAFGKKLKE RSIQEWQGYY INYKLMKKKV KQYSRQLEGG NLERRHVLKD FSRMLDNQIE
KIALFMLEQQ GLLASRLQTL RGSHDALQEQ PDISHMSYLK EEYRAVGQDL LKLLFFVEMN
AIGIRKILKK FDKRFGYRFT NYYVKTRANH PYSELQQVFR HVGLGAVVGA VSRNLHELQN
NQGSYLSIYD QPVLPLQDPV VDSIRAAVDR LTRSTNFLHF MAQHALIMQE ELPSPQDEEG
EEEDGRYHFM SLLLNLVNTF LYMVNTYIIV PTADDYSMSL GAAATVCGVV IGAMAVAQLF
SSVYFSAWSN RSYFKPLIFS SIVLFIGNLL YALAFDFNSI AVLLIGRLFC GLGSARAVNR
RYISDCVPLK IRMQASAGFV SASALGMACG PALAGLLQIR FKIYKLTFNQ DTLPGWVMAV
AWLIYLVWLA ISFREPAREP EEIPKTSEES NHSAVQDVNL EKGMKQPLLL TSEEIEEQGE
DECDGSEEAS EDSRTPANSI LAAYRLLTPS VKVQLLIYFM LKYAMEILLS ESSVITTYYF
GWSTSSVAIF LFCLGLTVLP VNLVVGSYIS NMFEDRQILL VSEIMVCVGI LLSFHVVVPY
TVPQYVCSGL IMFVSAEVLE GVNLSLLSRV MSSRLSRGTY NGGLLSTEAG TIARVIADAT
ITVAGFFGRN MLLNVTLLPS LVICVLSIVA TCFTYNSLY
