ID   SPX_LISMO               Reviewed;         131 AA.
AC   Q9RGX0; Q929I0;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-FEB-2004, sequence version 2.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Global transcriptional regulator Spx {ECO:0000255|HAMAP-Rule:MF_01132};
GN   Name=spx {ECO:0000255|HAMAP-Rule:MF_01132}; OrderedLocusNames=lmo2191;
OS   Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=169963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LO28 / Serovar 1/2c;
RX   PubMed=11004200; DOI=10.1128/jb.182.20.5931-5934.2000;
RA   Borezee E., Msadek T., Durant L., Berche P.;
RT   "Identification in Listeria monocytogenes of MecA, a homologue of the
RT   Bacillus subtilis competence regulatory protein.";
RL   J. Bacteriol. 182:5931-5934(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-679 / EGD-e;
RX   PubMed=11679669; DOI=10.1126/science.1063447;
RA   Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F.,
RA   Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A.,
RA   Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E.,
RA   Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O., Entian K.-D.,
RA   Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W.,
RA   Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U.,
RA   Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A.,
RA   Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B.,
RA   Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N.,
RA   Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT   "Comparative genomics of Listeria species.";
RL   Science 294:849-852(2001).
CC   -!- FUNCTION: Global transcriptional regulator that plays a key role in
CC       stress response and exerts either positive or negative regulation of
CC       genes. Acts by interacting with the C-terminal domain of the alpha
CC       subunit of the RNA polymerase (RNAP). This interaction can enhance
CC       binding of RNAP to the promoter region of target genes and stimulate
CC       their transcription, or block interaction of RNAP with activator.
CC       {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SUBUNIT: Interacts with the C-terminal domain of the alpha subunit of
CC       the RNAP. {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SIMILARITY: Belongs to the ArsC family. Spx subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01132}.
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DR   EMBL; AF103794; AAF21893.1; -; Genomic_DNA.
DR   EMBL; AL591982; CAD00269.1; -; Genomic_DNA.
DR   PIR; AG1348; AG1348.
DR   RefSeq; NP_465715.1; NC_003210.1.
DR   RefSeq; WP_003720569.1; NZ_CP023861.1.
DR   AlphaFoldDB; Q9RGX0; -.
DR   SMR; Q9RGX0; -.
DR   STRING; 169963.lmo2191; -.
DR   PaxDb; Q9RGX0; -.
DR   EnsemblBacteria; CAD00269; CAD00269; CAD00269.
DR   GeneID; 61190111; -.
DR   GeneID; 67410153; -.
DR   GeneID; 988006; -.
DR   KEGG; lmo:lmo2191; -.
DR   PATRIC; fig|169963.11.peg.2243; -.
DR   eggNOG; COG1393; Bacteria.
DR   HOGENOM; CLU_116644_1_1_9; -.
DR   OMA; IMIDDKR; -.
DR   PhylomeDB; Q9RGX0; -.
DR   BioCyc; LMON169963:LMO2191-MON; -.
DR   PHI-base; PHI:6967; -.
DR   Proteomes; UP000000817; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   HAMAP; MF_01132; Spx; 1.
DR   InterPro; IPR006660; Arsenate_reductase-like.
DR   InterPro; IPR023731; Spx.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   InterPro; IPR006504; Tscrpt_reg_Spx/MgsR.
DR   PANTHER; PTHR30041; PTHR30041; 1.
DR   Pfam; PF03960; ArsC; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   TIGRFAMs; TIGR01617; arsC_related; 1.
DR   PROSITE; PS51353; ARSC; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Disulfide bond; Redox-active center; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..131
FT                   /note="Global transcriptional regulator Spx"
FT                   /id="PRO_0000162559"
FT   DISULFID        10..13
FT                   /note="Redox-active"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01132"
FT   CONFLICT        16
FT                   /note="A -> S (in Ref. 1; AAF21893)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   131 AA;  15582 MW;  67181E1BA6CD9444 CRC64;
     MVTLYTSPSC TSCRKARAWL EEHDIPYKER NIFSEPLSLD EIKEILRMTE DGTDEIISTR
     SKTFQKLNVD LDSLPLQQLF ELIQKNPGLL RRPIIIDEKR LQVGYNEDEI RRFLPRRVRT
     YQLREAQKMV N