ID   SPX_STAAS               Reviewed;         131 AA.
AC   Q6GAS9;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Global transcriptional regulator Spx {ECO:0000255|HAMAP-Rule:MF_01132};
GN   Name=spx {ECO:0000255|HAMAP-Rule:MF_01132}; OrderedLocusNames=SAS0867;
OS   Staphylococcus aureus (strain MSSA476).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=282459;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MSSA476;
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA   Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA   Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA   Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA   Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA   Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT   for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC   -!- FUNCTION: Global transcriptional regulator that plays a key role in
CC       stress response and exerts either positive or negative regulation of
CC       genes. Acts by interacting with the C-terminal domain of the alpha
CC       subunit of the RNA polymerase (RNAP). This interaction can enhance
CC       binding of RNAP to the promoter region of target genes and stimulate
CC       their transcription, or block interaction of RNAP with activator.
CC       {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SUBUNIT: Interacts with the C-terminal domain of the alpha subunit of
CC       the RNAP. {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SIMILARITY: Belongs to the ArsC family. Spx subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01132}.
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DR   EMBL; BX571857; CAG42642.1; -; Genomic_DNA.
DR   RefSeq; WP_000258003.1; NC_002953.3.
DR   AlphaFoldDB; Q6GAS9; -.
DR   SMR; Q6GAS9; -.
DR   GeneID; 66839191; -.
DR   KEGG; sas:SAS0867; -.
DR   HOGENOM; CLU_116644_1_1_9; -.
DR   OMA; IMIDDKR; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   HAMAP; MF_01132; Spx; 1.
DR   InterPro; IPR006660; Arsenate_reductase-like.
DR   InterPro; IPR023731; Spx.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   InterPro; IPR006504; Tscrpt_reg_Spx/MgsR.
DR   PANTHER; PTHR30041; PTHR30041; 1.
DR   Pfam; PF03960; ArsC; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   TIGRFAMs; TIGR01617; arsC_related; 1.
DR   PROSITE; PS51353; ARSC; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Disulfide bond; Redox-active center; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..131
FT                   /note="Global transcriptional regulator Spx"
FT                   /id="PRO_0000162566"
FT   DISULFID        10..13
FT                   /note="Redox-active"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01132"
SQ   SEQUENCE   131 AA;  15441 MW;  89F67A54666699BF CRC64;
     MVTLFTSPSC TSCRKAKAWL QEHDIPYTER NIFSEHLTID EIKQILKMTE DGTDEIISTR
     SKTYQKLNVD IDSLPLQDLY SIIQDNPGLL RRPIILDNKR LQVGYNEDEI RRFLPRKVRT
     FQLQEAQRMV D