ID   SPX_STAS1               Reviewed;         131 AA.
AC   Q49WC6;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Global transcriptional regulator Spx {ECO:0000255|HAMAP-Rule:MF_01132};
GN   Name=spx {ECO:0000255|HAMAP-Rule:MF_01132}; OrderedLocusNames=SSP1788;
OS   Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS   20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=342451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX   PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA   Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA   Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT   "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT   pathogenesis of uncomplicated urinary tract infection.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
CC   -!- FUNCTION: Global transcriptional regulator that plays a key role in
CC       stress response and exerts either positive or negative regulation of
CC       genes. Acts by interacting with the C-terminal domain of the alpha
CC       subunit of the RNA polymerase (RNAP). This interaction can enhance
CC       binding of RNAP to the promoter region of target genes and stimulate
CC       their transcription, or block interaction of RNAP with activator.
CC       {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SUBUNIT: Interacts with the C-terminal domain of the alpha subunit of
CC       the RNAP. {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SIMILARITY: Belongs to the ArsC family. Spx subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01132}.
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DR   EMBL; AP008934; BAE18933.1; -; Genomic_DNA.
DR   RefSeq; WP_002483765.1; NZ_MTGA01000039.1.
DR   AlphaFoldDB; Q49WC6; -.
DR   SMR; Q49WC6; -.
DR   STRING; 342451.SSP1788; -.
DR   EnsemblBacteria; BAE18933; BAE18933; SSP1788.
DR   GeneID; 66867962; -.
DR   KEGG; ssp:SSP1788; -.
DR   eggNOG; COG1393; Bacteria.
DR   HOGENOM; CLU_116644_1_1_9; -.
DR   OMA; IMIDDKR; -.
DR   OrthoDB; 1606021at2; -.
DR   Proteomes; UP000006371; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   HAMAP; MF_01132; Spx; 1.
DR   InterPro; IPR006660; Arsenate_reductase-like.
DR   InterPro; IPR023731; Spx.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   InterPro; IPR006504; Tscrpt_reg_Spx/MgsR.
DR   PANTHER; PTHR30041; PTHR30041; 1.
DR   Pfam; PF03960; ArsC; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   TIGRFAMs; TIGR01617; arsC_related; 1.
DR   PROSITE; PS51353; ARSC; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Disulfide bond; Redox-active center; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..131
FT                   /note="Global transcriptional regulator Spx"
FT                   /id="PRO_0000162571"
FT   DISULFID        10..13
FT                   /note="Redox-active"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01132"
SQ   SEQUENCE   131 AA;  15440 MW;  89F9CDBC36748BBF CRC64;
     MVTLFTSPSC TSCRKAKAWL QEHDIPYTER NIFSEHLTID EIKQILKMTE DGTDEIISTR
     SKTYQKLNVD IDALPLQDLY SIIQDNPGLL RRPIILDEKR LQVGYNEDEI RRFLPRKVRT
     FQLQEAQRMV D