ID   SPX_STRP3               Reviewed;         134 AA.
AC   P0CZ82; Q8K7C0;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Global transcriptional regulator Spx {ECO:0000255|HAMAP-Rule:MF_01132};
GN   Name=spx {ECO:0000255|HAMAP-Rule:MF_01132}; OrderedLocusNames=SpyM3_0885;
OS   Streptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=198466;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-595 / MGAS315;
RX   PubMed=12122206; DOI=10.1073/pnas.152298499;
RA   Beres S.B., Sylva G.L., Barbian K.D., Lei B., Hoff J.S., Mammarella N.D.,
RA   Liu M.-Y., Smoot J.C., Porcella S.F., Parkins L.D., Campbell D.S.,
RA   Smith T.M., McCormick J.K., Leung D.Y.M., Schlievert P.M., Musser J.M.;
RT   "Genome sequence of a serotype M3 strain of group A Streptococcus: phage-
RT   encoded toxins, the high-virulence phenotype, and clone emergence.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:10078-10083(2002).
CC   -!- FUNCTION: Global transcriptional regulator that plays a key role in
CC       stress response and exerts either positive or negative regulation of
CC       genes. Acts by interacting with the C-terminal domain of the alpha
CC       subunit of the RNA polymerase (RNAP). This interaction can enhance
CC       binding of RNAP to the promoter region of target genes and stimulate
CC       their transcription, or block interaction of RNAP with activator.
CC       {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SUBUNIT: Interacts with the C-terminal domain of the alpha subunit of
CC       the RNAP. {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01132}.
CC   -!- SIMILARITY: Belongs to the ArsC family. Spx subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01132}.
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DR   EMBL; AE014074; AAM79492.1; -; Genomic_DNA.
DR   RefSeq; WP_002984496.1; NC_004070.1.
DR   AlphaFoldDB; P0CZ82; -.
DR   SMR; P0CZ82; -.
DR   EnsemblBacteria; AAM79492; AAM79492; SpyM3_0885.
DR   GeneID; 57852715; -.
DR   KEGG; spg:SpyM3_0885; -.
DR   HOGENOM; CLU_116644_1_1_9; -.
DR   OMA; IMIDDKR; -.
DR   Proteomes; UP000000564; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   HAMAP; MF_01132; Spx; 1.
DR   InterPro; IPR006660; Arsenate_reductase-like.
DR   InterPro; IPR023731; Spx.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   InterPro; IPR006504; Tscrpt_reg_Spx/MgsR.
DR   PANTHER; PTHR30041; PTHR30041; 1.
DR   Pfam; PF03960; ArsC; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   TIGRFAMs; TIGR01617; arsC_related; 1.
DR   PROSITE; PS51353; ARSC; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Disulfide bond; Redox-active center; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..134
FT                   /note="Global transcriptional regulator Spx"
FT                   /id="PRO_0000162578"
FT   DISULFID        10..13
FT                   /note="Redox-active"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01132"
SQ   SEQUENCE   134 AA;  15514 MW;  41549F88EC2E214E CRC64;
     MVTLFLSPSC TSCRKARAWL VKHEVDFQEH NIITSPLSRD ELMSILSFTE NGTEDIISTR
     SKVFQKLDVD VEELSISDLI DLIAKNPSLL RRPIIMDQKR MQIGFNEDEI RAFLSRDYRK
     QELRQATIKA EIEG