SPY1_HUMAN
ID SPY1_HUMAN Reviewed; 319 AA.
AC O43609; D3DNX6; Q6PNE0;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Protein sprouty homolog 1;
DE Short=Spry-1;
GN Name=SPRY1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=14533010;
RA Wang L., Ji C., Wu H., Xu J., Wu Q., Dai J., Yin G., Ye X., Gu S., Xie Y.,
RA Mao Y.;
RT "Identification and expression analysis of a novel splice variant of human
RT Sprouty1 gene.";
RL Int. J. Mol. Med. 12:783-787(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=PNS;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-319.
RA Raya A., de la Luna S.;
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 181-319.
RX PubMed=9458049; DOI=10.1016/s0092-8674(00)80919-8;
RA Hacohen N., Kramer S., Sutherland D., Hiromi Y., Krasnow M.A.;
RT "Sprouty encodes a novel antagonist of FGF signaling that patterns apical
RT branching of the Drosophila airways.";
RL Cell 92:253-263(1998).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Inhibits fibroblast growth factor (FGF)-induced retinal lens
CC fiber differentiation, probably by inhibiting FGF-mediated
CC phosphorylation of ERK1/2 (By similarity). Inhibits TGFB-induced
CC epithelial-to-mesenchymal transition in lens epithelial cells (By
CC similarity). {ECO:0000250|UniProtKB:Q9QXV9}.
CC -!- SUBUNIT: Forms heterodimers with SPRY2 (By similarity). Interacts with
CC TESK1 (By similarity). Interacts with CAV1 (via C-terminus) (By
CC similarity). {ECO:0000250|UniProtKB:Q9QXV9}.
CC -!- INTERACTION:
CC O43609; O43184-4: ADAM12; NbExp=4; IntAct=EBI-3866665, EBI-12006944;
CC O43609; Q6UY14-3: ADAMTSL4; NbExp=3; IntAct=EBI-3866665, EBI-10173507;
CC O43609; X5D778: ANKRD11; NbExp=3; IntAct=EBI-3866665, EBI-17183751;
CC O43609; Q2TAC2-2: CCDC57; NbExp=3; IntAct=EBI-3866665, EBI-10961624;
CC O43609; Q86X02: CDR2L; NbExp=3; IntAct=EBI-3866665, EBI-11063830;
CC O43609; Q9H2X0: CHRD; NbExp=3; IntAct=EBI-3866665, EBI-947551;
CC O43609; Q02930-3: CREB5; NbExp=8; IntAct=EBI-3866665, EBI-10192698;
CC O43609; A8MQ03: CYSRT1; NbExp=6; IntAct=EBI-3866665, EBI-3867333;
CC O43609; O95363: FARS2; NbExp=3; IntAct=EBI-3866665, EBI-2513774;
CC O43609; P23142-4: FBLN1; NbExp=3; IntAct=EBI-3866665, EBI-11956479;
CC O43609; Q5TD97: FHL5; NbExp=5; IntAct=EBI-3866665, EBI-750641;
CC O43609; P57678: GEMIN4; NbExp=4; IntAct=EBI-3866665, EBI-356700;
CC O43609; Q9Y223-2: GNE; NbExp=3; IntAct=EBI-3866665, EBI-11975289;
CC O43609; Q96NT3-2: GUCD1; NbExp=3; IntAct=EBI-3866665, EBI-11978177;
CC O43609; Q96MH2: HEXIM2; NbExp=3; IntAct=EBI-3866665, EBI-5460660;
CC O43609; P49639: HOXA1; NbExp=8; IntAct=EBI-3866665, EBI-740785;
CC O43609; O75031: HSF2BP; NbExp=3; IntAct=EBI-3866665, EBI-7116203;
CC O43609; Q0VD86: INCA1; NbExp=3; IntAct=EBI-3866665, EBI-6509505;
CC O43609; Q5T749: KPRP; NbExp=3; IntAct=EBI-3866665, EBI-10981970;
CC O43609; Q15323: KRT31; NbExp=3; IntAct=EBI-3866665, EBI-948001;
CC O43609; Q07627: KRTAP1-1; NbExp=3; IntAct=EBI-3866665, EBI-11959885;
CC O43609; Q8IUG1: KRTAP1-3; NbExp=3; IntAct=EBI-3866665, EBI-11749135;
CC O43609; P60412: KRTAP10-11; NbExp=3; IntAct=EBI-3866665, EBI-10217483;
CC O43609; P60370: KRTAP10-5; NbExp=3; IntAct=EBI-3866665, EBI-10172150;
CC O43609; P60409: KRTAP10-7; NbExp=6; IntAct=EBI-3866665, EBI-10172290;
CC O43609; P60410: KRTAP10-8; NbExp=6; IntAct=EBI-3866665, EBI-10171774;
CC O43609; P60411: KRTAP10-9; NbExp=6; IntAct=EBI-3866665, EBI-10172052;
CC O43609; Q8IUC1: KRTAP11-1; NbExp=3; IntAct=EBI-3866665, EBI-1052037;
CC O43609; P59991: KRTAP12-2; NbExp=3; IntAct=EBI-3866665, EBI-10176379;
CC O43609; P60328: KRTAP12-3; NbExp=3; IntAct=EBI-3866665, EBI-11953334;
CC O43609; Q9BYR7: KRTAP3-2; NbExp=3; IntAct=EBI-3866665, EBI-751260;
CC O43609; Q9BQ66: KRTAP4-12; NbExp=3; IntAct=EBI-3866665, EBI-739863;
CC O43609; Q9BYR5: KRTAP4-2; NbExp=3; IntAct=EBI-3866665, EBI-10172511;
CC O43609; P26371: KRTAP5-9; NbExp=3; IntAct=EBI-3866665, EBI-3958099;
CC O43609; Q9BYQ4: KRTAP9-2; NbExp=3; IntAct=EBI-3866665, EBI-1044640;
CC O43609; Q9BYQ3: KRTAP9-3; NbExp=3; IntAct=EBI-3866665, EBI-1043191;
CC O43609; Q5T7P2: LCE1A; NbExp=3; IntAct=EBI-3866665, EBI-11962058;
CC O43609; Q5T7P3: LCE1B; NbExp=3; IntAct=EBI-3866665, EBI-10245913;
CC O43609; Q5T751: LCE1C; NbExp=3; IntAct=EBI-3866665, EBI-12224199;
CC O43609; Q5T752: LCE1D; NbExp=3; IntAct=EBI-3866665, EBI-11741311;
CC O43609; Q5T754: LCE1F; NbExp=3; IntAct=EBI-3866665, EBI-11958008;
CC O43609; Q5TA79: LCE2A; NbExp=3; IntAct=EBI-3866665, EBI-10246607;
CC O43609; Q5TA81: LCE2C; NbExp=3; IntAct=EBI-3866665, EBI-11973993;
CC O43609; Q5TA82: LCE2D; NbExp=7; IntAct=EBI-3866665, EBI-10246750;
CC O43609; Q5TA76: LCE3A; NbExp=6; IntAct=EBI-3866665, EBI-9394625;
CC O43609; Q5T5A8: LCE3C; NbExp=3; IntAct=EBI-3866665, EBI-10245291;
CC O43609; Q9BYE3: LCE3D; NbExp=4; IntAct=EBI-3866665, EBI-6658837;
CC O43609; Q5TA78: LCE4A; NbExp=3; IntAct=EBI-3866665, EBI-10246358;
CC O43609; Q5TCM9: LCE5A; NbExp=3; IntAct=EBI-3866665, EBI-11955689;
CC O43609; Q8IYB1: MB21D2; NbExp=3; IntAct=EBI-3866665, EBI-11323212;
CC O43609; Q99750: MDFI; NbExp=7; IntAct=EBI-3866665, EBI-724076;
CC O43609; P50222: MEOX2; NbExp=4; IntAct=EBI-3866665, EBI-748397;
CC O43609; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-3866665, EBI-16439278;
CC O43609; Q7Z3S9: NOTCH2NLA; NbExp=5; IntAct=EBI-3866665, EBI-945833;
CC O43609; P0DPK4: NOTCH2NLC; NbExp=3; IntAct=EBI-3866665, EBI-22310682;
CC O43609; Q9P121-3: NTM; NbExp=3; IntAct=EBI-3866665, EBI-12027160;
CC O43609; P32242: OTX1; NbExp=4; IntAct=EBI-3866665, EBI-740446;
CC O43609; Q92824-2: PCSK5; NbExp=3; IntAct=EBI-3866665, EBI-11956269;
CC O43609; O15160: POLR1C; NbExp=3; IntAct=EBI-3866665, EBI-1055079;
CC O43609; P54646: PRKAA2; NbExp=3; IntAct=EBI-3866665, EBI-1383852;
CC O43609; O43741: PRKAB2; NbExp=3; IntAct=EBI-3866665, EBI-1053424;
CC O43609; Q9Y2K5-2: R3HDM2; NbExp=3; IntAct=EBI-3866665, EBI-10326419;
CC O43609; Q04864-2: REL; NbExp=3; IntAct=EBI-3866665, EBI-10829018;
CC O43609; P49901: SMCP; NbExp=3; IntAct=EBI-3866665, EBI-750494;
CC O43609; O43597: SPRY2; NbExp=3; IntAct=EBI-3866665, EBI-742487;
CC O43609; Q7Z6R9: TFAP2D; NbExp=3; IntAct=EBI-3866665, EBI-11952651;
CC O43609; Q96PF1: TGM7; NbExp=3; IntAct=EBI-3866665, EBI-12029034;
CC O43609; P57075-2: UBASH3A; NbExp=3; IntAct=EBI-3866665, EBI-7353612;
CC O43609; Q2TAL6: VWC2; NbExp=3; IntAct=EBI-3866665, EBI-11957238;
CC O43609; P17027: ZNF23; NbExp=4; IntAct=EBI-3866665, EBI-5657766;
CC O43609; Q9Y3S2: ZNF330; NbExp=3; IntAct=EBI-3866665, EBI-373456;
CC O43609; Q8TAU3: ZNF417; NbExp=3; IntAct=EBI-3866665, EBI-740727;
CC O43609; Q8N8Z8: ZNF441; NbExp=3; IntAct=EBI-3866665, EBI-17216366;
CC O43609; Q96EG3: ZNF837; NbExp=3; IntAct=EBI-3866665, EBI-11962574;
CC O43609; P09022: Hoxa1; Xeno; NbExp=3; IntAct=EBI-3866665, EBI-3957603;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein.
CC Note=Found in the cytoplasm in unstimulated cells but is translocated
CC to the membrane ruffles in cells stimulated with EGF (epidermal growth
CC factor).
CC -!- DOMAIN: The Cys-rich domain is responsible for the localization of the
CC protein to the membrane ruffles.
CC -!- SIMILARITY: Belongs to the sprouty family. {ECO:0000305}.
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DR EMBL; AY192146; AAP20102.1; -; mRNA.
DR EMBL; CH471056; EAX05209.1; -; Genomic_DNA.
DR EMBL; CH471056; EAX05210.1; -; Genomic_DNA.
DR EMBL; CH471056; EAX05211.1; -; Genomic_DNA.
DR EMBL; BC063856; AAH63856.1; -; mRNA.
DR EMBL; AY590694; AAT06102.1; -; mRNA.
DR EMBL; AF041037; AAC39566.1; -; mRNA.
DR CCDS; CCDS3731.1; -.
DR RefSeq; NP_001244967.1; NM_001258038.1.
DR RefSeq; NP_001244968.1; NM_001258039.1.
DR RefSeq; NP_005832.1; NM_005841.2.
DR RefSeq; NP_955359.1; NM_199327.2.
DR RefSeq; XP_005262743.1; XM_005262686.1.
DR RefSeq; XP_016863124.1; XM_017007635.1.
DR AlphaFoldDB; O43609; -.
DR SMR; O43609; -.
DR BioGRID; 115546; 110.
DR IntAct; O43609; 90.
DR MINT; O43609; -.
DR STRING; 9606.ENSP00000481675; -.
DR iPTMnet; O43609; -.
DR PhosphoSitePlus; O43609; -.
DR SwissPalm; O43609; -.
DR BioMuta; SPRY1; -.
DR EPD; O43609; -.
DR jPOST; O43609; -.
DR MassIVE; O43609; -.
DR MaxQB; O43609; -.
DR PaxDb; O43609; -.
DR PeptideAtlas; O43609; -.
DR PRIDE; O43609; -.
DR ProteomicsDB; 49078; -.
DR Antibodypedia; 26880; 275 antibodies from 31 providers.
DR DNASU; 10252; -.
DR Ensembl; ENST00000339241.1; ENSP00000343785.1; ENSG00000164056.11.
DR Ensembl; ENST00000394339.2; ENSP00000377871.2; ENSG00000164056.11.
DR Ensembl; ENST00000610581.4; ENSP00000481675.1; ENSG00000164056.11.
DR Ensembl; ENST00000622283.1; ENSP00000480540.1; ENSG00000164056.11.
DR Ensembl; ENST00000651917.1; ENSP00000498292.1; ENSG00000164056.11.
DR GeneID; 10252; -.
DR KEGG; hsa:10252; -.
DR MANE-Select; ENST00000651917.1; ENSP00000498292.1; NM_001258038.2; NP_001244967.1.
DR UCSC; uc003ifa.5; human.
DR CTD; 10252; -.
DR DisGeNET; 10252; -.
DR GeneCards; SPRY1; -.
DR HGNC; HGNC:11269; SPRY1.
DR HPA; ENSG00000164056; Tissue enhanced (adipose).
DR MIM; 602465; gene.
DR neXtProt; NX_O43609; -.
DR OpenTargets; ENSG00000164056; -.
DR PharmGKB; PA36098; -.
DR VEuPathDB; HostDB:ENSG00000164056; -.
DR eggNOG; ENOG502QSDN; Eukaryota.
DR GeneTree; ENSGT00950000183055; -.
DR HOGENOM; CLU_077696_0_0_1; -.
DR InParanoid; O43609; -.
DR OMA; AGHQFIC; -.
DR OrthoDB; 1157681at2759; -.
DR PhylomeDB; O43609; -.
DR TreeFam; TF325070; -.
DR PathwayCommons; O43609; -.
DR Reactome; R-HSA-182971; EGFR downregulation.
DR SignaLink; O43609; -.
DR SIGNOR; O43609; -.
DR BioGRID-ORCS; 10252; 9 hits in 1069 CRISPR screens.
DR ChiTaRS; SPRY1; human.
DR GeneWiki; SPRY1; -.
DR GenomeRNAi; 10252; -.
DR Pharos; O43609; Tbio.
DR PRO; PR:O43609; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; O43609; protein.
DR Bgee; ENSG00000164056; Expressed in pericardium and 188 other tissues.
DR ExpressionAtlas; O43609; baseline and differential.
DR Genevisible; O43609; HS.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005794; C:Golgi apparatus; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0048513; P:animal organ development; IBA:GO_Central.
DR GO; GO:0060449; P:bud elongation involved in lung branching; IEA:Ensembl.
DR GO; GO:0060940; P:epithelial to mesenchymal transition involved in cardiac fibroblast development; ISS:BHF-UCL.
DR GO; GO:0070371; P:ERK1 and ERK2 cascade; IEA:Ensembl.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; IEA:Ensembl.
DR GO; GO:0001656; P:metanephros development; IEA:Ensembl.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IEA:Ensembl.
DR GO; GO:0010719; P:negative regulation of epithelial to mesenchymal transition; ISS:UniProtKB.
DR GO; GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; ISS:UniProtKB.
DR GO; GO:0040037; P:negative regulation of fibroblast growth factor receptor signaling pathway; IMP:FlyBase.
DR GO; GO:0034260; P:negative regulation of GTPase activity; IEA:Ensembl.
DR GO; GO:1902747; P:negative regulation of lens fiber cell differentiation; ISS:UniProtKB.
DR GO; GO:0043407; P:negative regulation of MAP kinase activity; IBA:GO_Central.
DR GO; GO:0051387; P:negative regulation of neurotrophin TRK receptor signaling pathway; IEA:Ensembl.
DR GO; GO:0046580; P:negative regulation of Ras protein signal transduction; IBA:GO_Central.
DR GO; GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0001759; P:organ induction; IEA:Ensembl.
DR GO; GO:0001657; P:ureteric bud development; IEA:Ensembl.
DR InterPro; IPR007875; Sprouty.
DR InterPro; IPR030783; SPRY1.
DR PANTHER; PTHR12365:SF10; PTHR12365:SF10; 1.
DR Pfam; PF05210; Sprouty; 1.
DR PROSITE; PS51227; SPR; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Developmental protein; Membrane;
KW Reference proteome.
FT CHAIN 1..319
FT /note="Protein sprouty homolog 1"
FT /id="PRO_0000076898"
FT DOMAIN 183..295
FT /note="SPR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00572"
FT REGION 54..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 100..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 108..137
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 319 AA; 35122 MW; 40E2D4D12816E057 CRC64;
MDPQNQHGSG SSLVVIQQPS LDSRQRLDYE REIQPTAILS LDQIKAIRGS NEYTEGPSVV
KRPAPRTAPR QEKHERTHEI IPINVNNNYE HRHTSHLGHA VLPSNARGPI LSRSTSTGSA
ASSGSNSSAS SEQGLLGRSP PTRPVPGHRS ERAIRTQPKQ LIVDDLKGSL KEDLTQHKFI
CEQCGKCKCG ECTAPRTLPS CLACNRQCLC SAESMVEYGT CMCLVKGIFY HCSNDDEGDS
YSDNPCSCSQ SHCCSRYLCM GAMSLFLPCL LCYPPAKGCL KLCRRCYDWI HRPGCRCKNS
NTVYCKLESC PSRGQGKPS
