SPY2_CHICK
ID SPY2_CHICK Reviewed; 313 AA.
AC Q9PTL2;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Protein sprouty homolog 2;
DE Short=Spry-2;
GN Name=SPRY2;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION BY FGF.
RX PubMed=10498682; DOI=10.1242/dev.126.20.4465;
RA Minowada G., Jarvis L.A., Chi C.L., Neubueser A., Sun X., Hacohen N.,
RA Krasnow M.A., Martin G.R.;
RT "Vertebrate sprouty genes are induced by FGF signaling and can cause
RT chondrodysplasia when overexpressed.";
RL Development 126:4465-4475(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND INDUCTION BY
RP FGF.
RC TISSUE=Embryo;
RX PubMed=10662503; DOI=10.1006/mcne.1999.0801;
RA Chambers D., Medhurst A.D., Walsh F.S., Price J., Mason I.;
RT "Differential display of genes expressed at the midbrain-hindbrain junction
RT identifies sprouty2: an FGF8-inducible member of a family of intracellular
RT FGF antagonists.";
RL Mol. Cell. Neurosci. 15:22-35(2000).
CC -!- FUNCTION: Acts as an antagonist of FGF-induced retinal lens fiber
CC differentiation (By similarity). Inhibits TGFB-induced epithelial-to-
CC mesenchymal transition in retinal lens epithelial cells (By
CC similarity). May play an important role in FGF-mediated patterning of
CC the mid/hindbrain region by acting to modulate the signaling effects of
CC FGF8 (PubMed:10662503). {ECO:0000250|UniProtKB:Q9QXV8,
CC ECO:0000269|PubMed:10662503}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:O43597}.
CC Membrane {ECO:0000250|UniProtKB:O43597}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:O43597}. Note=Found in the cytoplasm in
CC unstimulated cells but is translocated to the membrane ruffles in cells
CC stimulated with EGF (epidermal growth factor).
CC {ECO:0000250|UniProtKB:O43597}.
CC -!- TISSUE SPECIFICITY: Brain and interlimb region.
CC -!- DEVELOPMENTAL STAGE: At the 4- to 5-somite stage (4/5S) found in the
CC embryo in scattered cells across the neural plate in the presumptive
CC mid/ hindbrain region (PubMed:10662503). At 7/8S found in the isthmus
CC and throughout the presumptive r1 territory. Between 10-14S stage found
CC throughout the R1 region and at the isthmic constriction
CC (PubMed:10662503). By 26S the anterior limit of expression extends into
CC the posterior midbrain region and this pattern of expression is
CC maintained at later stages (PubMed:10662503).
CC {ECO:0000269|PubMed:10662503}.
CC -!- INDUCTION: By FGF signaling. {ECO:0000269|PubMed:10498682,
CC ECO:0000269|PubMed:10662503}.
CC -!- DOMAIN: The Cys-rich domain is responsible for the localization of the
CC protein to the membrane ruffles.
CC -!- SIMILARITY: Belongs to the sprouty family. {ECO:0000305}.
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DR EMBL; AF176904; AAD56005.1; -; mRNA.
DR RefSeq; NP_990131.1; NM_204800.1.
DR AlphaFoldDB; Q9PTL2; -.
DR SMR; Q9PTL2; -.
DR STRING; 9031.ENSGALP00000027276; -.
DR PaxDb; Q9PTL2; -.
DR GeneID; 395584; -.
DR KEGG; gga:395584; -.
DR CTD; 10253; -.
DR VEuPathDB; HostDB:geneid_395584; -.
DR eggNOG; ENOG502QTG8; Eukaryota.
DR InParanoid; Q9PTL2; -.
DR OrthoDB; 1157681at2759; -.
DR PhylomeDB; Q9PTL2; -.
DR PRO; PR:Q9PTL2; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0048513; P:animal organ development; IBA:GO_Central.
DR GO; GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; IEA:InterPro.
DR GO; GO:0040037; P:negative regulation of fibroblast growth factor receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0043407; P:negative regulation of MAP kinase activity; IBA:GO_Central.
DR GO; GO:0046580; P:negative regulation of Ras protein signal transduction; IBA:GO_Central.
DR GO; GO:0045595; P:regulation of cell differentiation; IEA:InterPro.
DR GO; GO:0042127; P:regulation of cell population proliferation; IEA:InterPro.
DR InterPro; IPR007875; Sprouty.
DR InterPro; IPR030780; SPRY2.
DR PANTHER; PTHR12365:SF8; PTHR12365:SF8; 1.
DR Pfam; PF05210; Sprouty; 1.
DR PROSITE; PS51227; SPR; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Developmental protein; Membrane; Reference proteome.
FT CHAIN 1..313
FT /note="Protein sprouty homolog 2"
FT /id="PRO_0000076903"
FT DOMAIN 175..289
FT /note="SPR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00572"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 54..146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 96..138
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 313 AA; 34417 MW; 614C335E0DC36E83 CRC64;
METRVQHGSG SQALLQARRD SGRPHGEPDL RDVLTQQVHI LSLDQIRAIR NTNEYTEGPT
VAPRPGVKSA PRSASQPKSE RPHGLPEHRH FGRVQHTQTH ASPRAPLSRS ISTVSTGSRS
STRTSTSSNS SEQRLLGSSS GPVADGIVRM QPKSELKSSE LKPLSKEDLG AHSYRCEDCG
KCKCKECTYP RTLPSCWICD KQCLCSAQNV VDYGTCVCCV KGLFYHCSND DEDNCADNPC
SCSQSHCCTR WSAMGVVSLF LPCLWCYLPA KGCLKLCQGC YDRVNRPGCR CKHSNTVCCK
VPSVPPRNFE KPT
