SPY_ECO57
ID SPY_ECO57 Reviewed; 161 AA.
AC Q8XDZ4; Q7ADE9;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Periplasmic chaperone Spy {ECO:0000303|PubMed:21317898};
DE AltName: Full=Spheroplast protein Y;
DE Flags: Precursor;
GN Name=spy; OrderedLocusNames=ECs2449, Z2775;
GN ORFNames=AO055_07240, ASO15_12485;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WS4202;
RA Fellner L., Huptas C., Simon S., Krementowski A., Scherer S., Neuhaus K.;
RT "Draft genome sequence of three European lab-derivates from the
RT enterohemorrhagic E. coli O157:H7 strain EDL933, including two plasmids.";
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G10;
RA Staji H., Salehi T.Z., Orsini M., Tonelli A.;
RT "Genome sequence of Escherichia coli O157:H7 strains isolated from cattle
RT in Javadabad, Iran.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 52-147, FUNCTION, SUBUNIT, AND
RP DOMAIN.
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=21317898; DOI=10.1038/nsmb.2016;
RA Quan S., Koldewey P., Tapley T., Kirsch N., Ruane K.M., Pfizenmaier J.,
RA Shi R., Hofmann S., Foit L., Ren G., Jakob U., Xu Z., Cygler M.,
RA Bardwell J.C.;
RT "Genetic selection designed to stabilize proteins uncovers a chaperone
RT called Spy.";
RL Nat. Struct. Mol. Biol. 18:262-269(2011).
CC -!- FUNCTION: An ATP-independent periplasmic chaperone, decreases protein
CC aggregation and helps protein refolding. Binds substrate over a large
CC region. Protect proteins in vitro against tannin inactivation; tannins
CC have antimicrobial activity. Overexpression enhances the stability of
CC otherwise unstable periplasmic proteins (PubMed:21317898).
CC {ECO:0000305|PubMed:21317898}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:21317898}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305|PubMed:21317898}.
CC -!- DOMAIN: Has an elongated cradle shape. {ECO:0000269|PubMed:21317898}.
CC -!- SIMILARITY: Belongs to the CpxP/Spy family. {ECO:0000305}.
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DR EMBL; AE005174; AAG56729.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB35872.1; -; Genomic_DNA.
DR EMBL; CP012802; ALH90076.1; -; Genomic_DNA.
DR EMBL; LMXL01000076; KRQ10719.1; -; Genomic_DNA.
DR PIR; A99935; A99935.
DR PIR; E85783; E85783.
DR RefSeq; NP_310476.1; NC_002695.1.
DR RefSeq; WP_001228981.1; NZ_SWKA01000004.1.
DR PDB; 3O39; X-ray; 2.60 A; A/B=52-147.
DR PDBsum; 3O39; -.
DR AlphaFoldDB; Q8XDZ4; -.
DR SMR; Q8XDZ4; -.
DR STRING; 155864.EDL933_2702; -.
DR EnsemblBacteria; AAG56729; AAG56729; Z2775.
DR EnsemblBacteria; BAB35872; BAB35872; ECs_2449.
DR GeneID; 66674362; -.
DR GeneID; 913975; -.
DR KEGG; ece:Z2775; -.
DR KEGG; ecs:ECs_2449; -.
DR PATRIC; fig|386585.9.peg.2563; -.
DR eggNOG; COG3678; Bacteria.
DR HOGENOM; CLU_124352_1_0_6; -.
DR OMA; HDMMFKD; -.
DR EvolutionaryTrace; Q8XDZ4; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR CDD; cd09916; CpxP_like; 1.
DR InterPro; IPR012899; LTXXQ.
DR Pfam; PF07813; LTXXQ; 1.
DR PIRSF; PIRSF034445; CpxP_Spy; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Periplasm; Reference proteome; Signal;
KW Stress response.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..161
FT /note="Periplasmic chaperone Spy"
FT /id="PRO_5005941623"
FT REGION 141..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 59..70
FT /evidence="ECO:0007829|PDB:3O39"
FT TURN 71..74
FT /evidence="ECO:0007829|PDB:3O39"
FT HELIX 81..91
FT /evidence="ECO:0007829|PDB:3O39"
FT STRAND 92..95
FT /evidence="ECO:0007829|PDB:3O39"
FT HELIX 98..107
FT /evidence="ECO:0007829|PDB:3O39"
FT HELIX 109..127
FT /evidence="ECO:0007829|PDB:3O39"
FT HELIX 132..144
FT /evidence="ECO:0007829|PDB:3O39"
SQ SEQUENCE 161 AA; 18171 MW; B46C34867FC5E2AA CRC64;
MRKLTALFVA STLALGAANL AHAADTTTAA PADAKPMMHH KGKFGPHQDM MFKDLNLTDA
QKQQIREIMK GQRDQMKRPP LEERRAMHDI IASDTFDKAK AEAQIAKMEE QRKANMLAHM
ETQNKIYNIL TPEQKKQFNA NFEKRLTERP AAKGKMPATA E
