SPY_ORYSJ
ID SPY_ORYSJ Reviewed; 927 AA.
AC Q6YZI0; Q0J3Q0;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY;
DE EC=2.4.1.255 {ECO:0000250|UniProtKB:Q96301};
GN Name=SPY; OrderedLocusNames=Os08g0559300, LOC_Os08g44510;
GN ORFNames=P0562A06.20;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Probable O-linked N-acetylglucosamine transferase (OGT)
CC involved in various processes such as gibberellin (GA) signaling
CC pathway. OGTs catalyze the addition of nucleotide-activated sugars
CC directly onto the polypeptide through O-glycosidic linkage with the
CC hydroxyl of serine or threonine. Probably acts by adding O-linked
CC sugars to yet unknown proteins (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-
CC acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + H(+) + UDP;
CC Xref=Rhea:RHEA:48904, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:12251,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:57705,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:90838; EC=2.4.1.255;
CC Evidence={ECO:0000250|UniProtKB:Q96301};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-
CC (N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + H(+) + UDP;
CC Xref=Rhea:RHEA:48908, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:12252,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:57705,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:90840; EC=2.4.1.255;
CC Evidence={ECO:0000250|UniProtKB:Q96301};
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 41 family. O-GlcNAc
CC transferase subfamily. {ECO:0000305}.
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DR EMBL; AP005524; BAD13137.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF24415.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT06686.1; -; Genomic_DNA.
DR EMBL; AK065854; BAG89710.1; -; mRNA.
DR RefSeq; XP_015649746.1; XM_015794260.1.
DR AlphaFoldDB; Q6YZI0; -.
DR SMR; Q6YZI0; -.
DR STRING; 4530.OS08T0559300-01; -.
DR CAZy; GT41; Glycosyltransferase Family 41.
DR PaxDb; Q6YZI0; -.
DR PRIDE; Q6YZI0; -.
DR EnsemblPlants; Os08t0559300-01; Os08t0559300-01; Os08g0559300.
DR GeneID; 4346315; -.
DR Gramene; Os08t0559300-01; Os08t0559300-01; Os08g0559300.
DR KEGG; osa:4346315; -.
DR eggNOG; KOG4626; Eukaryota.
DR HOGENOM; CLU_001721_4_0_1; -.
DR InParanoid; Q6YZI0; -.
DR OMA; YQAHREW; -.
DR OrthoDB; 142546at2759; -.
DR UniPathway; UPA00378; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR Genevisible; Q6YZI0; OS.
DR GO; GO:0005737; C:cytoplasm; IEA:EnsemblPlants.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0097363; F:protein O-GlcNAc transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:EnsemblPlants.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009938; P:negative regulation of gibberellic acid mediated signaling pathway; IEA:EnsemblPlants.
DR GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR GO; GO:2000377; P:regulation of reactive oxygen species metabolic process; IEA:EnsemblPlants.
DR Gene3D; 1.25.40.10; -; 4.
DR InterPro; IPR029489; OGT/SEC/SPY_C.
DR InterPro; IPR006597; Sel1-like.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR001440; TPR_1.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF13844; Glyco_transf_41; 2.
DR Pfam; PF00515; TPR_1; 5.
DR SMART; SM00671; SEL1; 3.
DR SMART; SM00028; TPR; 11.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS50005; TPR; 11.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 2: Evidence at transcript level;
KW Gibberellin signaling pathway; Glycosyltransferase; Nucleus;
KW Reference proteome; Repeat; TPR repeat; Transferase.
FT CHAIN 1..927
FT /note="Probable UDP-N-acetylglucosamine--peptide N-
FT acetylglucosaminyltransferase SPINDLY"
FT /id="PRO_0000191780"
FT REPEAT 34..67
FT /note="TPR 1"
FT REPEAT 68..101
FT /note="TPR 2"
FT REPEAT 102..135
FT /note="TPR 3"
FT REPEAT 143..176
FT /note="TPR 4"
FT REPEAT 177..210
FT /note="TPR 5"
FT REPEAT 211..244
FT /note="TPR 6"
FT REPEAT 252..285
FT /note="TPR 7"
FT REPEAT 286..319
FT /note="TPR 8"
FT REPEAT 320..353
FT /note="TPR 9"
FT REPEAT 355..387
FT /note="TPR 10"
FT REPEAT 388..421
FT /note="TPR 11"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 422..927
FT /note="Catalytic region"
SQ SEQUENCE 927 AA; 102548 MW; 83607C53C42AE6AA CRC64;
MGRPGMDSSE GRESNGVVPE RNGGAVPAKQ QLDGKDTLRY ANILRSRNKF AEALQLYNNV
LEKDEANVEA LIGKGICLQA QSLPMQAIEC FNEAVRIDPG NACALTYCGM IYKDEGHLVE
AAEAYQKARN ADPSYKPAAE FLAIVLTDLG TSLKLAGNTE EGIQKYCEAL EVDSHYAPAY
YNLGVVYSEM MQFDLALTCY EKAALERPLY AEAYCNMGVI YKNRGELEAA IACYERCLTI
SPNFEIAKNN MAIALTDLGT KVKIEGDINQ GVAYYKKALF YNWHYADAMY NLGVAYGEML
NFEMAIVFYE LALHFNPRCA EACNNLGVIY KDRDNLDKAV ECYQMALSIK PNFSQSLNNL
GVVYTVQGKM DAASSMIQKA IFANSTYAEA YNNLGVLYRD AGSITSAVQA YEKCLQIDPD
SRNAGQNRLL ALNYIDEGFD DKLYQAHREW GKRFLKLYPQ YTSWDNPKVA DRPLVIGYVS
PDYFTHSVSY FIEAPLAHHD YSNYKVVVYS GVVKADAKTL RFKDKVLKKG GLWRDIYGID
EKKVASLVRE DKVDILVELT GHTANNKLGT MACRPAPIQV TWIGYPNTTG LPTIDYRITD
SLADPPDTTQ KHVEELVRLP ESFLCYSPSP EAGPVCPTPA ILNGFITFGS FNNLAKITPK
VLQVWAKILC AVPNSRLVVK CKPFCCDSIR QKFLSTLAEL GLEPLRVDLL PLIHLNHDHM
QAYSLMDISL DTFPYAGTTT TCESLYMGVP CVTMAGSVHA HNVGVSLLTK VGLGRLVAKS
ENEYVSLALD LAADVTALQE LRMSLRGLMA KSPVCDGENF TRGLESAYRN MWRRYCDGDA
PALRRLDLLQ EEPCSNNNKQ DFDDNQVAKL ADLKAQRVDA AVDGDKQSQL TAHAAVVGEV
QQAPIMVNGV SSPVSSGKVE ANGHISR
