SPZ1_MOUSE
ID SPZ1_MOUSE Reviewed; 378 AA.
AC Q99MY0; Q3V487; Q80X69;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Spermatogenic leucine zipper protein 1;
DE AltName: Full=BHLH-Zip transcription factor SPZ1;
DE AltName: Full=Spermatogenic Zip 1;
GN Name=Spz1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INDUCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=11165476; DOI=10.1016/s0925-4773(00)00513-x;
RA Hsu S.-H., Shyu H.-W., Hsieh-Li H.-M., Li H.;
RT "Spz1, a novel bHLH-Zip protein, is specifically expressed in testis.";
RL Mech. Dev. 100:177-187(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH PPP1CC ISOFORM GAMMA-2.
RX PubMed=15226296; DOI=10.1074/jbc.m403710200;
RA Hrabchak C., Varmuza S.;
RT "Identification of the spermatogenic zip protein Spz1 as a putative protein
RT phosphatase-1 (PP1) regulatory protein that specifically binds the
RT PP1cgamma2 splice variant in mouse testis.";
RL J. Biol. Chem. 279:37079-37086(2004).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=14980513; DOI=10.1016/j.yexcr.2003.10.029;
RA Hsu S.-H., Hsieh-Li H.-M., Li H.;
RT "Dysfunctional spermatogenesis in transgenic mice overexpressing bHLH-Zip
RT transcription factor, Spz1.";
RL Exp. Cell Res. 294:185-198(2004).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND PHOSPHORYLATION.
RX PubMed=15899793; DOI=10.1158/0008-5472.can-04-3658;
RA Hsu S.-H., Hsieh-Li H.-M., Huang H.-Y., Huang P.-H., Li H.;
RT "bHLH-zip transcription factor Spz1 mediates mitogen-activated protein
RT kinase cell proliferation, transformation, and tumorigenesis.";
RL Cancer Res. 65:4041-4050(2005).
CC -!- FUNCTION: Transcription factor that binds to the DNA sequence 5'-
CC CANNTG-3'(E box) and the G-box motif. Directly binds to a guanine-rich
CC region of the PCNA promoter and up-regulates its expression which in
CC turn induces cell transformation and tumor formation. May play an
CC important role in the regulation of cell proliferation and
CC differentiation during spermatogenesis. {ECO:0000269|PubMed:11165476,
CC ECO:0000269|PubMed:14980513, ECO:0000269|PubMed:15899793}.
CC -!- SUBUNIT: Interacts with PPP1CC isoform gamma-2. This interaction can
CC prevent SPZ1 binding to the E-box and inhibits PPP1CC activity.
CC {ECO:0000269|PubMed:15226296}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15226296}. Nucleus
CC {ECO:0000269|PubMed:11165476, ECO:0000269|PubMed:15226296}.
CC -!- TISSUE SPECIFICITY: Expressed specifically in the testis and
CC epidydimis. In the testis expressed in both germ cells and somatic
CC cells (Sertoli and Leydig cells). Expressed in several tumor cell
CC lines. {ECO:0000269|PubMed:11165476, ECO:0000269|PubMed:14980513,
CC ECO:0000269|PubMed:15899793}.
CC -!- DEVELOPMENTAL STAGE: First detected in the testis at postnatal day 10
CC and levels increase further and reach highest levels at day 40 and then
CC gradually decrease. {ECO:0000269|PubMed:11165476}.
CC -!- INDUCTION: Down-regulated by testosterone and retinoic acid.
CC {ECO:0000269|PubMed:11165476}.
CC -!- PTM: Phosphorylated by MAPK1/ERK2 and MAPK3/ERK1.
CC {ECO:0000269|PubMed:15899793}.
CC -!- MISCELLANEOUS: The helix-loop-helix and basic motifs form a Spz1
CC specific bHLH different from the classical one.
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DR EMBL; AF271697; AAK15458.1; -; mRNA.
DR EMBL; AK006437; BAE43195.1; -; mRNA.
DR EMBL; BC050748; AAH50748.1; -; mRNA.
DR CCDS; CCDS26683.1; -.
DR RefSeq; NP_084513.3; NM_030237.3.
DR AlphaFoldDB; Q99MY0; -.
DR SMR; Q99MY0; -.
DR STRING; 10090.ENSMUSP00000054083; -.
DR PhosphoSitePlus; Q99MY0; -.
DR PaxDb; Q99MY0; -.
DR PRIDE; Q99MY0; -.
DR ProteomicsDB; 261582; -.
DR Antibodypedia; 44374; 118 antibodies from 24 providers.
DR DNASU; 79401; -.
DR Ensembl; ENSMUST00000050658; ENSMUSP00000054083; ENSMUSG00000046957.
DR GeneID; 79401; -.
DR KEGG; mmu:79401; -.
DR UCSC; uc007rkr.2; mouse.
DR CTD; 84654; -.
DR MGI; MGI:1930801; Spz1.
DR VEuPathDB; HostDB:ENSMUSG00000046957; -.
DR eggNOG; ENOG502QS87; Eukaryota.
DR GeneTree; ENSGT00950000182767; -.
DR HOGENOM; CLU_062447_0_0_1; -.
DR InParanoid; Q99MY0; -.
DR OMA; FNIHVAR; -.
DR OrthoDB; 1269936at2759; -.
DR PhylomeDB; Q99MY0; -.
DR TreeFam; TF337798; -.
DR BioGRID-ORCS; 79401; 4 hits in 70 CRISPR screens.
DR PRO; PR:Q99MY0; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q99MY0; protein.
DR Bgee; ENSMUSG00000046957; Expressed in seminiferous tubule of testis and 15 other tissues.
DR Genevisible; Q99MY0; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:MGI.
DR InterPro; IPR042961; Spz1.
DR PANTHER; PTHR47889; PTHR47889; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; DNA-binding; Nucleus; Phosphoprotein;
KW Proto-oncogene; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..378
FT /note="Spermatogenic leucine zipper protein 1"
FT /id="PRO_0000280508"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 116..127
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255"
FT REGION 116..122
FT /note="Interaction with PPP1CC isoform gamma-2"
FT REGION 128..193
FT /note="Basic motif"
FT /evidence="ECO:0000255"
FT REGION 252..273
FT /note="Leucine-zipper"
FT COILED 182..233
FT /evidence="ECO:0000255"
FT MOD_RES 106
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6AXY9"
FT MOD_RES 207
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6AXY9"
FT CONFLICT 261
FT /note="H -> Q (in Ref. 2; BAE43195)"
FT /evidence="ECO:0000305"
FT CONFLICT 297
FT /note="L -> P (in Ref. 3; AAH50748)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 378 AA; 43091 MW; EB83F5A921046672 CRC64;
MSDTDNSAEM PARCPSPNPA PGAKQEPPNS GITISLLEIG SLPTVCYHSF PPPKNSICPV
EKRGRVQKFS NLLKDVKDVL KNIAGVEEKS TVGEPFDDAY IPEDLSELNV RGVEKKNKIR
FKDDLFIHFD PEREQNTMKQ MLLKNQSAKN MVPKFARDLC NAEETRGFDG MLLSVKRPRN
GSLHLRGEYR KLRNNMEQLL QEADHWSKQH NELSELMRSY QECQNETQET TDKDRACLQN
QPNNGLSTKQ KLEEQVKKLS HDTHALHLIA ALLENECQVL QQRVDILKDF HLHEAGLGHE
KPLQMSCEQD KKCPKLAEAD KTDAFKHTTR ATEGTIRKPK ILRSPDVCFT KKARNNRFNA
RVAKKSLVGK RRTVSSFR
