SPZ3_DROME
ID SPZ3_DROME Reviewed; 611 AA.
AC Q9VLV7; Q8WTE9;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Protein spaetzle 3 {ECO:0000303|PubMed:11536362};
DE AltName: Full=Protein spatzle 3 {ECO:0000312|FlyBase:FBgn0031959};
DE Flags: Precursor;
GN Name=spz3 {ECO:0000312|FlyBase:FBgn0031959};
GN ORFNames=CG7104 {ECO:0000312|FlyBase:FBgn0031959};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|EMBL:AAL33883.1}
RP NUCLEOTIDE SEQUENCE [MRNA], AND IDENTIFICATION.
RX PubMed=11536362; DOI=10.1002/prot.1125;
RA Parker J.S., Mizuguchi K., Gay N.J.;
RT "A family of proteins related to Spaetzle, the toll receptor ligand, are
RT encoded in the Drosophila genome.";
RL Proteins 45:71-80(2001).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|EMBL:AAM52001.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM52001.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAM52001.1};
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.;
RL Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=24662564; DOI=10.1083/jcb.201308115;
RA Ballard S.L., Miller D.L., Ganetzky B.;
RT "Retrograde neurotrophin signaling through Tollo regulates synaptic growth
RT in Drosophila.";
RL J. Cell Biol. 204:1157-1172(2014).
CC -!- FUNCTION: Neurotrophin which may function as a ligand to the Toll-
CC related receptor Tollo. Involved in a Tollo and JNK signaling pathway
CC that positively regulates neuromuscular junction (NMJ) growth in
CC presynaptic motorneurons. May function by activating Tollo to promote
CC the phosphorylation of JNK. {ECO:0000269|PubMed:24662564}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000250|UniProtKB:P48607}.
CC -!- DEVELOPMENTAL STAGE: Expressed in larval muscles.
CC {ECO:0000269|PubMed:24662564}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown has no effect on
CC neuromuscular junction (NMJ) growth. However, conditional knockdown in
CC the muscles results in a decrease in bouton number at the NMJs.
CC {ECO:0000269|PubMed:24662564}.
CC -!- MISCELLANEOUS: 'Spaetzle' means 'noodles' in German. {ECO:0000305}.
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DR EMBL; AF296285; AAL33883.1; -; mRNA.
DR EMBL; AE014134; AAF52574.2; -; Genomic_DNA.
DR EMBL; AY121674; AAM52001.1; -; mRNA.
DR RefSeq; NP_609160.2; NM_135316.3.
DR AlphaFoldDB; Q9VLV7; -.
DR IntAct; Q9VLV7; 2.
DR STRING; 7227.FBpp0079183; -.
DR GlyGen; Q9VLV7; 4 sites.
DR PaxDb; Q9VLV7; -.
DR DNASU; 34077; -.
DR EnsemblMetazoa; FBtr0079561; FBpp0079183; FBgn0031959.
DR GeneID; 34077; -.
DR KEGG; dme:Dmel_CG7104; -.
DR UCSC; CG7104-RA; d. melanogaster.
DR CTD; 34077; -.
DR FlyBase; FBgn0031959; spz3.
DR VEuPathDB; VectorBase:FBgn0031959; -.
DR eggNOG; ENOG502QWM3; Eukaryota.
DR HOGENOM; CLU_035541_1_0_1; -.
DR InParanoid; Q9VLV7; -.
DR OMA; KNNPYGP; -.
DR OrthoDB; 844699at2759; -.
DR PhylomeDB; Q9VLV7; -.
DR BioGRID-ORCS; 34077; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34077; -.
DR PRO; PR:Q9VLV7; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031959; Expressed in wing disc and 11 other tissues.
DR GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0008083; F:growth factor activity; IBA:GO_Central.
DR GO; GO:0005121; F:Toll binding; IBA:GO_Central.
DR GO; GO:0021556; P:central nervous system formation; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR GO; GO:0008592; P:regulation of Toll signaling pathway; IBA:GO_Central.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR032104; Spaetzle.
DR Pfam; PF16077; Spaetzle; 1.
DR SUPFAM; SSF57501; SSF57501; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Reference proteome; Signal.
FT SIGNAL 1..14
FT /evidence="ECO:0000255"
FT CHAIN 15..611
FT /note="Protein spaetzle 3"
FT /evidence="ECO:0000303|PubMed:11536362"
FT /id="PRO_0000437665"
FT DOMAIN 521..609
FT /note="Spaetzle"
FT /evidence="ECO:0000255"
FT REGION 57..322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 477..518
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..159
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 167..217
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..243
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..307
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 491..518
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 5
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 335
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 351
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 511
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 522..573
FT /evidence="ECO:0000250|UniProtKB:P48607"
FT DISULFID 559..605
FT /evidence="ECO:0000250|UniProtKB:P48607"
FT DISULFID 567..607
FT /evidence="ECO:0000250|UniProtKB:P48607"
FT DISULFID 604
FT /note="Interchain"
FT /evidence="ECO:0000250|UniProtKB:P48607"
SQ SEQUENCE 611 AA; 67126 MW; 67B6C56AC3E47E8A CRC64;
MALTNFSLPF GALGQPWGVT IAPLHPIHQL ASNTNNLLYS PADHQQQTPA EAAADPEYFK
NNPYAPPQSG GYQYQNTAGR RKQSNAYLPP TAPNAVRNSV YHIQQVQQTQ QQQTQQQHQQ
QDQHENSVSF QSSSSRSSSS STTGQSSIQL TQTHASGRGP AEGSYSRYPG QQAQPPQQQQ
PQQKQYFNAH GSASATFTKN SGSFSITSFG SRQQQQQPPQ PQQPPPSQQQ QPPPAPPPQR
SRQAKPEAQP AQTYGVAPPE NYPERAPGFT RVQAGQGSRT QVHAVLDYDV EEGEEDEEED
GEEEGQFYEG QENDKSNNNQ MPTVTPIQGP IYLKNGTVPV VPLFSYPKLN NGSFLQIPIW
WTALSVALGL DVRGDVIKGV PCIKRYHQLF CPTAGNSYPI DKIERFIDDN KALMRRMYGD
FEMNMEGPGG GGGRQQGKVR KRRFIDEPDI FIPPGAFAAN AGETVEAGDS YFGQLRKKRQ
AAAGGSRNRG GSAGGSGNGN TNANRQPGNK NGSSGTGRLD ACESKIEIVT PYWASNSAGK
IRAIVNTQHF EQAIHQEVCS NTQTPRCEGE CGCEQKYKWH RLLAYDPDND CKGIFMDWFL
FPSCCVCRCN P
