SPZ4_DROME
ID SPZ4_DROME Reviewed; 597 AA.
AC Q9VKG9; D0IQH0;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Protein spaetzle 4 {ECO:0000303|PubMed:11536362};
DE AltName: Full=Protein spatzle 4 {ECO:0000312|FlyBase:FBgn0032362};
DE Flags: Precursor;
GN Name=spz4 {ECO:0000312|FlyBase:FBgn0032362};
GN ORFNames=CG14928 {ECO:0000312|FlyBase:FBgn0032362};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:ACY46083.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Carlson J., Booth B., Frise E., Sandler J., Wan K., Yu C., Celniker S.;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP IDENTIFICATION, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=11536362; DOI=10.1002/prot.1125;
RA Parker J.S., Mizuguchi K., Gay N.J.;
RT "A family of proteins related to Spaetzle, the toll receptor ligand, are
RT encoded in the Drosophila genome.";
RL Proteins 45:71-80(2001).
CC -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000250|UniProtKB:P48607}.
CC -!- DEVELOPMENTAL STAGE: Detected in larvae and adults.
CC {ECO:0000269|PubMed:11536362}.
CC -!- INDUCTION: Transiently down-regulated by microbial infection.
CC {ECO:0000269|PubMed:11536362}.
CC -!- MISCELLANEOUS: 'Spaetzle' means 'noodles' in German. {ECO:0000305}.
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DR EMBL; AE014134; AAF53100.2; -; Genomic_DNA.
DR EMBL; BT100208; ACY46083.1; -; mRNA.
DR RefSeq; NP_609504.2; NM_135660.3.
DR AlphaFoldDB; Q9VKG9; -.
DR IntAct; Q9VKG9; 1.
DR STRING; 7227.FBpp0293785; -.
DR GlyGen; Q9VKG9; 4 sites.
DR PaxDb; Q9VKG9; -.
DR DNASU; 34572; -.
DR EnsemblMetazoa; FBtr0305261; FBpp0293785; FBgn0032362.
DR GeneID; 34572; -.
DR KEGG; dme:Dmel_CG14928; -.
DR UCSC; CG14928-RA; d. melanogaster.
DR CTD; 34572; -.
DR FlyBase; FBgn0032362; spz4.
DR VEuPathDB; VectorBase:FBgn0032362; -.
DR eggNOG; ENOG502R7GT; Eukaryota.
DR HOGENOM; CLU_533434_0_0_1; -.
DR InParanoid; Q9VKG9; -.
DR OMA; SSCICKC; -.
DR OrthoDB; 844699at2759; -.
DR PhylomeDB; Q9VKG9; -.
DR BioGRID-ORCS; 34572; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34572; -.
DR PRO; PR:Q9VKG9; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032362; Expressed in crop (Drosophila) and 3 other tissues.
DR GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0008083; F:growth factor activity; IBA:GO_Central.
DR GO; GO:0005121; F:Toll binding; IBA:GO_Central.
DR GO; GO:0021556; P:central nervous system formation; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR GO; GO:0008592; P:regulation of Toll signaling pathway; IBA:GO_Central.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR032104; Spaetzle.
DR Pfam; PF16077; Spaetzle; 1.
DR SUPFAM; SSF57501; SSF57501; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Disulfide bond; Glycoprotein; Immunity; Reference proteome;
KW Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..597
FT /note="Protein spaetzle 4"
FT /evidence="ECO:0000303|PubMed:11536362"
FT /id="PRO_0000437522"
FT DOMAIN 448..538
FT /note="Spaetzle"
FT /evidence="ECO:0000255"
FT REGION 179..235
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 289..340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 390..419
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 107..135
FT /evidence="ECO:0000255"
FT COMPBIAS 179..224
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..314
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 325..340
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 215
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 287
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 354
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 463
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 450..502
FT /evidence="ECO:0000250|UniProtKB:P48607"
FT DISULFID 487..534
FT /evidence="ECO:0000250|UniProtKB:P48607"
FT DISULFID 496..536
FT /evidence="ECO:0000250|UniProtKB:P48607"
SQ SEQUENCE 597 AA; 65755 MW; 4B16D97114BED4B8 CRC64;
MVRTQMDRRS PITRLITIEV IGYLLILMTI ALPLSSAGFG FDSANSCSPN GKISRRGRAQ
ILAAIPCDLG QQAFCHLPGS AYPWHAVRRF VHENQGLMKR MYGDVRHISI LRDEIQNNEV
DADDIEETAE RYSKDGGRRS AKYLMNNRDR DREREDFGSF KSNDVLMEPH FRPVSTITTS
TTKATTTTTT APEIVSSTTE RVPNEVTDTT PGNTNSTIST TKGTAPPLHE KPESETDIVE
IQPSPESNSV YEVVSPSVLS TTSTAKPSSA AGENIQIIDS PQLGLRNLSA EASPSQEPPT
ATTGKPTSVP KSTTPLPIRK RKPAETTTSA ASPKVSSTIS TPTTTTATLL IRRNVTKYSP
ASVTTPVSFA SLFSGTSSGL FSPERLRRPL TTTSSTSAPA SSPPAGGTPA SSSTGSASKS
GLLREGQLFQ DAMKQEPVAV ASNLRGVNAC PVKDEVVAPF WANNTRGEVL ALLNLYPFEQ
YVHWEKCTHE FKQMFCRDGC RCEQQYRLHR LLAYDPHNEC RGIFSDWFRF PSSCICKCYN
IPMEFRATSR SPRSDSRFDA PKSDPIEAAE AEVQRAIYEH ATEEWYRPRD EFDFLED
