位置:首页 > 蛋白库 > SQHC_BACSU
SQHC_BACSU
ID   SQHC_BACSU              Reviewed;         632 AA.
AC   Q796C3; O34650;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Sporulenol synthase;
DE            EC=4.2.1.137;
DE   AltName: Full=Tetraprenyl-beta-curcumene cyclase;
GN   Name=sqhC; OrderedLocusNames=BSU19320;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT   "Sequence analysis of the Bacillus subtilis chromosome region between the
RT   terC and odhAB loci cloned in a yeast artificial chromosome.";
RL   Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=20713508; DOI=10.1101/gad.1945010;
RA   Lopez D., Kolter R.;
RT   "Functional microdomains in bacterial membranes.";
RL   Genes Dev. 24:1893-1902(2010).
RN   [4]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=21627333; DOI=10.1021/ja203779h;
RA   Sato T., Yoshida S., Hoshino H., Tanno M., Nakajima M., Hoshino T.;
RT   "Sesquarterpenes (C35 terpenes) biosynthesized via the cyclization of a
RT   linear C35 isoprenoid by a tetraprenyl-beta-curcumene synthase and a
RT   tetraprenyl-beta-curcumene cyclase: identification of a new terpene
RT   cyclase.";
RL   J. Am. Chem. Soc. 133:9734-9737(2011).
CC   -!- FUNCTION: Catalyzes the cyclization of tetraprenyl beta-curcumene into
CC       sporulenol. {ECO:0000269|PubMed:21627333}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=sporulenol = (R)-tetraprenyl-beta-curcumene + H2O;
CC         Xref=Rhea:RHEA:34255, ChEBI:CHEBI:15377, ChEBI:CHEBI:64801,
CC         ChEBI:CHEBI:67182; EC=4.2.1.137;
CC         Evidence={ECO:0000269|PubMed:21627333};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; hopanoid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: No effect on biofilm formation.
CC       {ECO:0000269|PubMed:20713508}.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB84441.1; Type=Frameshift; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF027868; AAB84441.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AL009126; CAB13824.2; -; Genomic_DNA.
DR   PIR; A69718; A69718.
DR   RefSeq; NP_389814.2; NC_000964.3.
DR   RefSeq; WP_004399534.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; Q796C3; -.
DR   SMR; Q796C3; -.
DR   STRING; 224308.BSU19320; -.
DR   PaxDb; Q796C3; -.
DR   EnsemblBacteria; CAB13824; CAB13824; BSU_19320.
DR   GeneID; 939443; -.
DR   KEGG; bsu:BSU19320; -.
DR   PATRIC; fig|224308.179.peg.2113; -.
DR   eggNOG; COG1657; Bacteria.
DR   InParanoid; Q796C3; -.
DR   OMA; CWARQTI; -.
DR   PhylomeDB; Q796C3; -.
DR   BioCyc; BSUB:BSU19320-MON; -.
DR   BRENDA; 4.2.1.137; 658.
DR   UniPathway; UPA00337; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016866; F:intramolecular transferase activity; IEA:InterPro.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Lyase; Membrane; Reference proteome; Repeat.
FT   CHAIN           1..632
FT                   /note="Sporulenol synthase"
FT                   /id="PRO_0000360070"
FT   REPEAT          395..436
FT                   /note="PFTB 1"
FT   REPEAT          465..505
FT                   /note="PFTB 2"
FT   REPEAT          513..554
FT                   /note="PFTB 3"
FT   ACT_SITE        377
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   632 AA;  71224 MW;  C79E4090386832F6 CRC64;
     MGTLQEKVRR FQKKTITELR DRQNADGSWT FCFEGPIMTN SFFILLLTSL DEGENEKELI
     SSLAAGIHAK QQPDGTFINY PDETRGNLTA TVQGYVGMLA SGCFHRTEPH MKKAEQFIIS
     HGGLRHVHFM TKWMLAANGL YPWPALYLPL SLMALPPTLP IHFYQFSSYA RIHFAPMAVT
     LNQRFVLINR NISSLHHLDP HMTKNPFTWL RSDAFEERDL TSILLHWKRV FHAPFAFQQL
     GLQTAKTYML DRIEKDGTLY SYASATIYMV YSLLSLGVSR YSPIIRRAIT GIKSLVTKCN
     GIPYLENSTS TVWDTALISY ALQKNGVTET DGSVTKAADF LLERQHTKIA DWSVKNPNSV
     PGGWGFSNIN TNNPDCDDTT AVLKAIPRNH SPAAWERGVS WLLSMQNNDG GFSAFEKNVN
     HPLIRLLPLE SAEDAAVDPS TADLTGRVLH FLGEKVGFTE KHQHIQRAVK WLFEHQEQNG
     SWYGRWGVCY IYGTWAALTG MHACGVDRKH PGIQKALRWL KSIQNDDGSW GESCKSAEIK
     TYVPLHRGTI VQTAWALDAL LTYENSEHPS VVKGMQYLTD SSSHSADSLA YPAGIGLPKQ
     FYIRYHSYPY VFSLLAVGKY LDSIEKETAN ET
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024