ID   SQHC_BRADU              Reviewed;         660 AA.
AC   P54924;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2003, sequence version 2.
DT   25-MAY-2022, entry version 127.
DE   RecName: Full=Squalene--hopene cyclase;
DE            EC=4.2.1.129;
DE            EC=5.4.99.17;
DE   AltName: Full=Squalene--hopanol cyclase;
GN   Name=shc; OrderedLocusNames=blr3004;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Perzl M., Poralla K.;
RL   Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Catalyzes the cyclization of squalene into hopene.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=squalene = hop-22(29)-ene; Xref=Rhea:RHEA:17637,
CC         ChEBI:CHEBI:4648, ChEBI:CHEBI:15440; EC=5.4.99.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + squalene = hopan-22-ol; Xref=Rhea:RHEA:16561,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15440, ChEBI:CHEBI:36484;
CC         EC=4.2.1.129;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; hopanoid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; X86552; CAA60250.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC48269.1; -; Genomic_DNA.
DR   RefSeq; NP_769644.1; NC_004463.1.
DR   RefSeq; WP_011085788.1; NZ_CP011360.1.
DR   AlphaFoldDB; P54924; -.
DR   SMR; P54924; -.
DR   STRING; 224911.27351262; -.
DR   EnsemblBacteria; BAC48269; BAC48269; BAC48269.
DR   GeneID; 64022754; -.
DR   KEGG; bja:blr3004; -.
DR   PATRIC; fig|224911.44.peg.2630; -.
DR   eggNOG; COG1657; Bacteria.
DR   HOGENOM; CLU_019345_0_0_5; -.
DR   InParanoid; P54924; -.
DR   OMA; CWARQTI; -.
DR   PhylomeDB; P54924; -.
DR   UniPathway; UPA00337; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0051007; F:squalene-hopene cyclase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR006400; Hopene-cyclase.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01507; hopene_cyclase; 1.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Isomerase; Lyase; Membrane; Reference proteome; Repeat.
FT   CHAIN           1..660
FT                   /note="Squalene--hopene cyclase"
FT                   /id="PRO_0000072651"
FT   REPEAT          73..114
FT                   /note="PFTB 1"
FT   REPEAT          419..460
FT                   /note="PFTB 2"
FT   REPEAT          536..586
FT                   /note="PFTB 3"
FT   ACT_SITE        394
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
FT   CONFLICT        7..10
FT                   /note="TARE -> PP (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        347
FT                   /note="V -> G (in Ref. 1; CAA60250)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        557
FT                   /note="A -> T (in Ref. 1; CAA60250)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        641
FT                   /note="A -> G (in Ref. 1; CAA60250)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   660 AA;  73702 MW;  FC3BBC9903959F66 CRC64;
     MDSVNATARE AKESKISESE ILESSIASAT QGVLGFQQSD GHWVFELEAD CTIPAEYVLL
     RHYLAEPVDT VLEAKIGNYL RRVQGAHGGW PLVHDGEFDM SASVKAYFAL KMIGDSIDAP
     HMVRAREAIH ARGGAIHSNV FTRFMLAMFG IVTWRAVPVL PIEIMLLPFW SPFHINKISY
     WARTTMVPLM VIAALKPRAK NPKGVGIDEL FLQDPRSIGM TAKAPHQSMA WFLLFRSLDA
     ILRVIEPLFP KSLRKRAIDT ALAFSEERLN GEDGMGAIYP PMANLVMMYD ALGKDENYPP
     RAVTRRGIDK LLVIGDDEAY CQPCVSPVWD TTLTAHALLE AGGDKAVPAA KHGLDWLIPK
     QELEVKGDWA VKRPDVRPGG WAFQYNNAYY PDLDDTAVVV MSMDRMRREH GVTGYDSAID
     RGREWIEGMQ SDDGGWAAFD VNNLEYYLNN IPFSDHGALL DPPTEDVTAR CVSMLAQLGE
     TAKTSKHVAD GVAYLRKTQH PEGSWYGRWG MNFIYGTWSV LCALNMAGVR HDDPMIRKAA
     DWLASIQNKD GGWGEDAVSY RLDYKGWEAA PSTASQTAWA LLALMAAGEV DHPAVARGVE
     YLIATQNEKG LWDEQRYTAT GFPRVFYLRY HGYSKFFPLW ALARYRNLRN TNSRVVGVGM