ID   SQHC_SINFN              Reviewed;         647 AA.
AC   P55348;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 130.
DE   RecName: Full=Probable squalene--hopene cyclase;
DE            EC=4.2.1.129;
DE            EC=5.4.99.17;
DE   AltName: Full=Squalene--hopanol cyclase;
GN   Name=shc; OrderedLocusNames=NGR_a00460; ORFNames=y4aA;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: Catalyzes the cyclization of squalene into hopene. Probably
CC       part of an operon y4aABCD involved in the synthesis of an isoprenoid
CC       compound.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=squalene = hop-22(29)-ene; Xref=Rhea:RHEA:17637,
CC         ChEBI:CHEBI:4648, ChEBI:CHEBI:15440; EC=5.4.99.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + squalene = hopan-22-ol; Xref=Rhea:RHEA:16561,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15440, ChEBI:CHEBI:36484;
CC         EC=4.2.1.129;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; hopanoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; U00090; AAB91964.1; -; Genomic_DNA.
DR   RefSeq; NP_443761.1; NC_000914.2.
DR   AlphaFoldDB; P55348; -.
DR   SMR; P55348; -.
DR   STRING; 394.NGR_a00460; -.
DR   EnsemblBacteria; AAB91964; AAB91964; NGR_a00460.
DR   KEGG; rhi:NGR_a00460; -.
DR   PATRIC; fig|394.7.peg.43; -.
DR   eggNOG; COG1657; Bacteria.
DR   HOGENOM; CLU_019345_0_0_5; -.
DR   OMA; CWARQTI; -.
DR   OrthoDB; 260889at2; -.
DR   UniPathway; UPA00337; -.
DR   Proteomes; UP000001054; Plasmid sym pNGR234a.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0051007; F:squalene-hopene cyclase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR006400; Hopene-cyclase.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01507; hopene_cyclase; 1.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   3: Inferred from homology;
KW   Isomerase; Lyase; Plasmid; Reference proteome; Repeat.
FT   CHAIN           1..647
FT                   /note="Probable squalene--hopene cyclase"
FT                   /id="PRO_0000072652"
FT   REPEAT          67..108
FT                   /note="PFTB 1"
FT   REPEAT          413..454
FT                   /note="PFTB 2"
FT   REPEAT          530..577
FT                   /note="PFTB 3"
FT   ACT_SITE        388
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   647 AA;  72130 MW;  BD0C336905D1ECAE CRC64;
     MNKHSGNRTA IDPAALEMSI ASATEALLAY RHADGHWAFE LEADSTIPSE YILLRHYLAE
     PIDVVLEAKI GNYLRRTQGA HGGWPLVHDG PFDMSASVKS YFALKMIGDS VDAAHMVKAR
     EAIRARGGAA NSNVLTRFLL ALYGVVSWRA VPVLPIEIVL LPIWSPFHLY KISYWARTTI
     VPLMVLAVLK PRAKNPKGVG IEELFLQDTK SVGMNPKAPH QSWGWFLLFR GIDGILRVIE
     PHLPKKLRER AIASALAFTE ERLNGEDGMG AIYPSMANIV MMYDALGKDD HFPPRAIARR
     AIDKLLVIGE EEAYCQPCLS PVWDTALTCH ALQEVGGANA VAKAKQGLDW LKPRQVLDVK
     GDWAVKAPNI RPGGWPFQYN NAHYPDLDDT AVVVMAMDRA QRHAGSKEYA TAIARGREWI
     EGMQSRDGGW AAFDVNNLEY YLNNLPFADH GALLDPPTED VTARCVSMLA QVGEFTQRSK
     AVAEGIAYLR RTQHAEGSWY GRWGLNYIYG TWSVLCALNA AGIDHQDPMI RKAVEWLVSI
     QSWDGGWGED AISYRLDYSG YEQAPSTSSQ TAWALLGLMA AGEVEHPAVA RGVNYLKNAQ
     TENGLWDEQR YTATGFPRVF YLRYHGYSKF FPLWALARYR NLRSTNV