SQHC_ZYMMO
ID SQHC_ZYMMO Reviewed; 658 AA.
AC P33990; Q5NP64;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 2.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=Squalene--hopene cyclase;
DE EC=4.2.1.129;
DE EC=5.4.99.17;
DE AltName: Full=Squalene--hopanol cyclase;
GN Name=shc; OrderedLocusNames=ZMO0872;
OS Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC Zymomonadaceae; Zymomonas.
OX NCBI_TaxID=264203;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 31821 / ZM4 / CP4;
RX PubMed=7894707; DOI=10.1099/00221287-141-1-155;
RA Reipen I.G., Poralla K., Sahm H., Sprenger G.A.;
RT "Zymomonas mobilis squalene-hopene cyclase gene (shc): cloning, DNA
RT sequence analysis, and expression in Escherichia coli.";
RL Microbiology 141:155-161(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 31821 / ZM4 / CP4;
RA Reipen I.G., Sahm H., Sprenger G.A.;
RL Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 31821 / ZM4 / CP4;
RA Um H.W.;
RL Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 31821 / ZM4 / CP4;
RX PubMed=15592456; DOI=10.1038/nbt1045;
RA Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H.,
RA Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J.,
RA Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y., Kang H.L., Lee S.Y., Lee K.J.,
RA Kang H.S.;
RT "The genome sequence of the ethanologenic bacterium Zymomonas mobilis
RT ZM4.";
RL Nat. Biotechnol. 23:63-68(2005).
CC -!- FUNCTION: Catalyzes the cyclization of squalene into hopene.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=squalene = hop-22(29)-ene; Xref=Rhea:RHEA:17637,
CC ChEBI:CHEBI:4648, ChEBI:CHEBI:15440; EC=5.4.99.17;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + squalene = hopan-22-ol; Xref=Rhea:RHEA:16561,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15440, ChEBI:CHEBI:36484;
CC EC=4.2.1.129;
CC -!- PATHWAY: Secondary metabolite biosynthesis; hopanoid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC {ECO:0000305}.
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DR EMBL; X80766; CAA56749.1; -; Genomic_DNA.
DR EMBL; X73561; CAA51958.1; -; Genomic_DNA.
DR EMBL; AJ001401; CAA04735.1; -; Genomic_DNA.
DR EMBL; AF203881; AAF12829.1; -; Genomic_DNA.
DR EMBL; AE008692; AAV89496.1; -; Genomic_DNA.
DR PIR; S37494; S37494.
DR RefSeq; WP_011240738.1; NZ_CP035711.1.
DR AlphaFoldDB; P33990; -.
DR SMR; P33990; -.
DR STRING; 264203.ZMO0872; -.
DR PRIDE; P33990; -.
DR EnsemblBacteria; AAV89496; AAV89496; ZMO0872.
DR GeneID; 58026676; -.
DR KEGG; zmo:ZMO0872; -.
DR eggNOG; COG1657; Bacteria.
DR HOGENOM; CLU_019345_0_0_5; -.
DR OMA; CWARQTI; -.
DR OrthoDB; 260889at2; -.
DR BRENDA; 5.4.99.17; 6765.
DR UniPathway; UPA00337; -.
DR Proteomes; UP000001173; Chromosome.
DR GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0051007; F:squalene-hopene cyclase activity; IEA:UniProtKB-EC.
DR GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd02892; SQCY_1; 1.
DR InterPro; IPR006400; Hopene-cyclase.
DR InterPro; IPR032696; SQ_cyclase_C.
DR InterPro; IPR032697; SQ_cyclase_N.
DR InterPro; IPR018333; Squalene_cyclase.
DR InterPro; IPR002365; Terpene_synthase_CS.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR11764; PTHR11764; 1.
DR Pfam; PF13243; SQHop_cyclase_C; 1.
DR Pfam; PF13249; SQHop_cyclase_N; 1.
DR SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR TIGRFAMs; TIGR01507; hopene_cyclase; 1.
DR TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE 3: Inferred from homology;
KW Cell membrane; Isomerase; Lyase; Membrane; Reference proteome; Repeat.
FT CHAIN 1..658
FT /note="Squalene--hopene cyclase"
FT /id="PRO_0000072653"
FT REPEAT 69..110
FT /note="PFTB 1"
FT REPEAT 418..459
FT /note="PFTB 2"
FT REPEAT 486..526
FT /note="PFTB 3"
FT REPEAT 534..584
FT /note="PFTB 4"
FT ACT_SITE 392
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P48449"
FT CONFLICT 34
FT /note="H -> Q (in Ref. 1, 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 64
FT /note="Q -> E (in Ref. 1, 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 411
FT /note="R -> H (in Ref. 1, 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 534
FT /note="I -> V (in Ref. 1, 2 and 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 658 AA; 74109 MW; 42D2D8FC06EA1D9C CRC64;
MTVSTSSAFH HSPLSDDVEP IIQKATRALL EKQHQDGHWV FELEADATIP AEYILLKHYL
GEPQDLEIEA KIGRYLRRIQ GEHGGWSLFY GGDLDLSATV KAYFALKMIG DSPDAPHMLR
ARNEILARGG AMRANVFTRI QLALFGAMSW EHVPQMPVEL MLMPEWFPVH INKMAYWART
VLVPLLVLQA LKPVARNRRG ILVDELFVPD VLPTLQESGD PIWRRFFSAL DKVLHKVEPY
WPKNMRAKAI HSCVHFVTER LNGEDGLGAI YPAIANSVMM YDALGYPENH PERAIARRAV
EKLMVLDGTE DQGDKEVYCQ PCLSPIWDTA LVAHAMLEVG GDEAEKSAIS ALSWLKPQQI
LDVKGDWAWR RPDLRPGGWA FQYRNDYYPD VDDTAVVTMA MDRAAKLSDL RDDFEESKAR
AMEWTIGMQS DNGGWGAFDA NNSYTYLNNI PFADHGALLD PPTVDVSARC VSMMAQAGIS
ITDPKMKAAV DYLLKEQEED GSWFGRWGVN YIYGTWSALC ALNVAALPHD HLAIQKAVAW
LKTIQNEDGG WGENCDSYAL DYSGYEPMDS TASQTAWALL GLMAVGEANS EAVTKGINWL
AQNQDEEGLW KEDYYSGGGF PRVFYLRYHG YSKYFPLWAL ARYRNLKKAN QPIVHYGM
