SQT1_YEAST
ID SQT1_YEAST Reviewed; 431 AA.
AC P35184; D6VVU2;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Ribosome assembly protein SQT1;
GN Name=SQT1; OrderedLocusNames=YIR012W; ORFNames=YIB12W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9271392; DOI=10.1128/mcb.17.9.5146;
RA Eisinger D.P., Dick F.A., Denke E., Trumpower B.L.;
RT "SQT1, which encodes an essential WD domain protein of Saccharomyces
RT cerevisiae, suppresses dominant-negative mutations of the ribosomal protein
RT gene QSR1.";
RL Mol. Cell. Biol. 17:5146-5155(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7762303; DOI=10.1002/yea.320110109;
RA Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S.,
RA Schwager C., Zimmermann J., Sander C., Ansorge W.;
RT "Nucleotide sequence and analysis of the centromeric region of yeast
RT chromosome IX.";
RL Yeast 11:61-78(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-224.
RX PubMed=7925484;
RA Liang S., Lacroute F., Kepes F.;
RT "Multicopy STS1 restores both protein transport and ribosomal RNA stability
RT in a new yeast sec23 mutant allele.";
RL Eur. J. Cell Biol. 62:270-281(1993).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: May be involved in the late step of 60S ribosomal subunit
CC assembly or modification in the cytoplasm.
CC -!- SUBUNIT: Interacts strongly with QSR1. Part of an oligomeric protein
CC complex that is loosely associated with ribosomes.
CC -!- MISCELLANEOUS: Present with 19700 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
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DR EMBL; U75717; AAB69630.1; -; Genomic_DNA.
DR EMBL; X79743; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; Z37996; CAA86082.1; -; Genomic_DNA.
DR EMBL; X75916; CAA53516.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006942; DAA08558.1; -; Genomic_DNA.
DR PIR; S48356; S48356.
DR RefSeq; NP_012277.1; NM_001179534.1.
DR PDB; 4ZOV; X-ray; 2.00 A; A/B=53-431.
DR PDB; 4ZOX; X-ray; 1.60 A; A=53-431.
DR PDB; 5AMS; X-ray; 3.35 A; A/B=1-431.
DR PDBsum; 4ZOV; -.
DR PDBsum; 4ZOX; -.
DR PDBsum; 5AMS; -.
DR AlphaFoldDB; P35184; -.
DR SMR; P35184; -.
DR BioGRID; 35004; 59.
DR DIP; DIP-2508N; -.
DR IntAct; P35184; 16.
DR MINT; P35184; -.
DR STRING; 4932.YIR012W; -.
DR iPTMnet; P35184; -.
DR MaxQB; P35184; -.
DR PaxDb; P35184; -.
DR PRIDE; P35184; -.
DR EnsemblFungi; YIR012W_mRNA; YIR012W; YIR012W.
DR GeneID; 854829; -.
DR KEGG; sce:YIR012W; -.
DR SGD; S000001451; SQT1.
DR VEuPathDB; FungiDB:YIR012W; -.
DR eggNOG; KOG0296; Eukaryota.
DR GeneTree; ENSGT00940000153648; -.
DR HOGENOM; CLU_000288_57_9_1; -.
DR InParanoid; P35184; -.
DR OMA; VWCYQIN; -.
DR BioCyc; YEAST:G3O-31433-MON; -.
DR PRO; PR:P35184; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P35184; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0051082; F:unfolded protein binding; IDA:SGD.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IMP:SGD.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IMP:SGD.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..431
FT /note="Ribosome assembly protein SQT1"
FT /id="PRO_0000051227"
FT REPEAT 63..102
FT /note="WD 1"
FT REPEAT 107..146
FT /note="WD 2"
FT REPEAT 149..192
FT /note="WD 3"
FT REPEAT 199..243
FT /note="WD 4"
FT REPEAT 309..348
FT /note="WD 5"
FT REPEAT 350..387
FT /note="WD 6"
FT STRAND 58..61
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 68..73
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 75..84
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 89..98
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 101..105
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 112..117
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 121..128
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 131..139
FT /evidence="ECO:0007829|PDB:4ZOX"
FT HELIX 140..142
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 144..152
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 157..162
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 164..166
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 169..174
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 179..184
FT /evidence="ECO:0007829|PDB:4ZOX"
FT TURN 186..188
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 191..197
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 204..210
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 220..225
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 230..234
FT /evidence="ECO:0007829|PDB:4ZOX"
FT TURN 235..237
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 240..244
FT /evidence="ECO:0007829|PDB:4ZOX"
FT HELIX 246..248
FT /evidence="ECO:0007829|PDB:4ZOX"
FT TURN 249..251
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 256..261
FT /evidence="ECO:0007829|PDB:4ZOX"
FT HELIX 264..266
FT /evidence="ECO:0007829|PDB:4ZOX"
FT TURN 267..269
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 273..278
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 281..287
FT /evidence="ECO:0007829|PDB:4ZOX"
FT TURN 288..292
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 294..299
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 305..307
FT /evidence="ECO:0007829|PDB:5AMS"
FT HELIX 309..312
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 314..320
FT /evidence="ECO:0007829|PDB:4ZOX"
FT TURN 321..324
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 325..330
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 333..339
FT /evidence="ECO:0007829|PDB:4ZOX"
FT TURN 340..342
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 345..350
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 355..361
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 364..369
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 374..378
FT /evidence="ECO:0007829|PDB:4ZOX"
FT TURN 379..381
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 384..388
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 395..401
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 413..419
FT /evidence="ECO:0007829|PDB:4ZOX"
FT STRAND 424..429
FT /evidence="ECO:0007829|PDB:4ZOX"
SQ SEQUENCE 431 AA; 47167 MW; 981D566076B0C881 CRC64;
MEPQEEFITT EEVEQEIVPT VEVEQDVPVD IEGENDDDDE MMNDDEEALE VDMSNNSLTY
FDKHTDSVFA IGHHPNLPLV CTGGGDNLAH LWTSHSQPPK FAGTLTGYGE SVISCSFTSE
GGFLVTADMS GKVLVHMGQK GGAQWKLASQ MQEVEEIVWL KTHPTIARTF AFGATDGSVW
CYQINEQDGS LEQLMSGFVH QQDCSMGEFI NTDKGENTLE LVTCSLDSTI VAWNCFTGQQ
LFKITQAEIK GLEAPWISLS LAPETLTKGN SGVVACGSNN GLLAVINCNN GGAILHLSTV
IELKPEQDEL DASIESISWS SKFSLMAIGL VCGEILLYDT SAWRVRHKFV LEDSVTKLMF
DNDDLFASCI NGKVYQFNAR TGQEKFVCVG HNMGVLDFIL LHPVANTGTE QKRKVITAGD
EGVSLVFEVP N
