SQV7_CAEEL
ID SQV7_CAEEL Reviewed; 329 AA.
AC Q18779;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=UDP-sugar transporter sqv-7;
DE AltName: Full=Squashed vulva protein 7;
GN Name=sqv-7; ORFNames=C52E12.3;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND MUTAGENESIS OF GLY-95 AND LEU-151.
RX PubMed=9927677; DOI=10.1073/pnas.96.3.968;
RA Herman T., Hartwieg E., Horvitz H.R.;
RT "sqv mutants of Caenorhabditis elegans are defective in vulval epithelial
RT invagination.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:968-973(1999).
RN [3]
RP FUNCTION, AND MUTAGENESIS OF GLY-95.
RX PubMed=11005858; DOI=10.1073/pnas.97.20.10838;
RA Bulik D.A., Wei G., Toyoda H., Kinoshita-Toyoda A., Waldrip W.R.,
RA Esko J.D., Robbins P.W., Selleck S.B.;
RT "sqv-3, -7, and -8, a set of genes affecting morphogenesis in
RT Caenorhabditis elegans, encode enzymes required for glycosaminoglycan
RT biosynthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:10838-10843(2000).
RN [4]
RP FUNCTION.
RX PubMed=11259660; DOI=10.1073/pnas.061593098;
RA Berninsone P., Hwang H.Y., Zemtseva I., Horvitz H.R., Hirschberg C.B.;
RT "SQV-7, a protein involved in Caenorhabditis elegans epithelial
RT invagination and early embryogenesis, transports UDP-glucuronic acid, UDP-
RT N-acetylgalactosamine, and UDP-galactose.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:3738-3743(2001).
RN [5]
RP FUNCTION.
RX PubMed=24052309; DOI=10.1126/science.1242528;
RA Pedersen M.E., Snieckute G., Kagias K., Nehammer C., Multhaupt H.A.,
RA Couchman J.R., Pocock R.;
RT "An epidermal microRNA regulates neuronal migration through control of the
RT cellular glycosylation state.";
RL Science 341:1404-1408(2013).
CC -!- FUNCTION: Involved in the import of UDP-glucuronic acid (UDP-GlcA),
CC UDP-N-acetylgalactosamine (UDP-GalNAc) and UDP-galactose (UDP-Gal) from
CC the cytoplasm into the Golgi lumen for the biosynthesis of
CC glycoconjugates that play a pivotal role in development
CC (PubMed:11259660). Involved in the synthesis of chondroitin sulfate and
CC heparan sulfate proteoglycans (PubMed:11005858). Required for embryonic
CC development (PubMed:9927677). Involved in vulva epithelium invagination
CC and embryonic development (PubMed:9927677, PubMed:11259660). Involved
CC in the directed migration of hermaphrodite-specific neurons
CC (PubMed:24052309). {ECO:0000269|PubMed:11005858,
CC ECO:0000269|PubMed:11259660, ECO:0000269|PubMed:24052309,
CC ECO:0000269|PubMed:9927677}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass membrane
CC protein.
CC -!- SIMILARITY: Belongs to the TPT transporter family. SLC35D subfamily.
CC {ECO:0000305}.
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DR EMBL; FO080554; CCD64624.1; -; Genomic_DNA.
DR PIR; T15823; T15823.
DR RefSeq; NP_495436.1; NM_063035.5.
DR AlphaFoldDB; Q18779; -.
DR SMR; Q18779; -.
DR STRING; 6239.C52E12.3.1; -.
DR TCDB; 2.A.7.15.2; the drug/metabolite transporter (dmt) superfamily.
DR EPD; Q18779; -.
DR PaxDb; Q18779; -.
DR EnsemblMetazoa; C52E12.3.1; C52E12.3.1; WBGene00005025.
DR EnsemblMetazoa; C52E12.3.2; C52E12.3.2; WBGene00005025.
DR GeneID; 174145; -.
DR KEGG; cel:CELE_C52E12.3; -.
DR UCSC; C52E12.3; c. elegans.
DR CTD; 174145; -.
DR WormBase; C52E12.3; CE04263; WBGene00005025; sqv-7.
DR eggNOG; KOG1444; Eukaryota.
DR GeneTree; ENSGT00940000167969; -.
DR HOGENOM; CLU_040726_1_0_1; -.
DR InParanoid; Q18779; -.
DR OMA; IQSTVCV; -.
DR OrthoDB; 1093260at2759; -.
DR PhylomeDB; Q18779; -.
DR Reactome; R-CEL-173599; Formation of the active cofactor, UDP-glucuronate.
DR Reactome; R-CEL-2022854; Keratan sulfate biosynthesis.
DR Reactome; R-CEL-2022928; HS-GAG biosynthesis.
DR Reactome; R-CEL-727802; Transport of nucleotide sugars.
DR PRO; PR:Q18779; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00005025; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; IDA:WormBase.
DR GO; GO:0005461; F:UDP-glucuronic acid transmembrane transporter activity; IDA:WormBase.
DR GO; GO:0005463; F:UDP-N-acetylgalactosamine transmembrane transporter activity; IDA:WormBase.
DR GO; GO:0005462; F:UDP-N-acetylglucosamine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0030206; P:chondroitin sulfate biosynthetic process; IMP:UniProtKB.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0035262; P:gonad morphogenesis; IMP:UniProtKB.
DR GO; GO:0015014; P:heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process; IMP:UniProtKB.
DR GO; GO:0002009; P:morphogenesis of an epithelium; IMP:WormBase.
DR GO; GO:0018991; P:oviposition; IMP:WormBase.
DR GO; GO:1903354; P:regulation of distal tip cell migration; IMP:UniProtKB.
DR GO; GO:0000003; P:reproduction; IMP:WormBase.
DR GO; GO:0072334; P:UDP-galactose transmembrane transport; IDA:WormBase.
DR GO; GO:0015787; P:UDP-glucuronic acid transmembrane transport; IDA:WormBase.
DR GO; GO:0015789; P:UDP-N-acetylgalactosamine transmembrane transport; IDA:WormBase.
DR GO; GO:0040025; P:vulval development; IMP:WormBase.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR Pfam; PF03151; TPT; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Membrane; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..329
FT /note="UDP-sugar transporter sqv-7"
FT /id="PRO_0000213395"
FT TRANSMEM 15..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 95
FT /note="G->D: In n2839; 50 percent reduction in brood size.
FT Partial collapse of vulva invagination. Reduced levels of
FT chondroitin and heparan sulfate proteoglycans associated
FT with a reduction in the chondroitin chain length."
FT /evidence="ECO:0000269|PubMed:11005858,
FT ECO:0000269|PubMed:9927677"
FT MUTAGEN 151
FT /note="L->P: In n2844; F1 adults accumulate eggs in the
FT uterus. Embryos are predominantly arrested at the
FT bean/comma embryonic stage."
FT /evidence="ECO:0000269|PubMed:9927677"
SQ SEQUENCE 329 AA; 36893 MW; 152080BD2F80109B CRC64;
MTSTVQSPLY SRVFSAVFYG VISVLIVFVN KILLTNYKFP SFLFVGVGQM MATILILFFA
KMFRIVQFPS LDSSIPRKIM PLPLLYFFNL ISGLGGTQMI NLPMFTVLRR FSILMTMILE
FYILNVKASK AVKISVGLMI GGSFIAAIYD LSFDALGYTM IFINNICTAA LGVYTKQKLD
AKDLGKYGLM FYNCLFMLLP ALCVVQYTGD LDRAYSFMLS DSMTSSVWTC FLLSCICGFV
LNYSLVLCTH HNSALTTTCV GPIKNLFVTY VGMFSSGDYV FQWANFTGIN VSVFGSILYT
YVTFRSKSTT ISYKPLPMTM PIDVHKPRN
