SR34A_ARATH
ID SR34A_ARATH Reviewed; 300 AA.
AC A2RVS6; A0A1I9LMJ8; A8MR30; Q9CA06; Q9SCL3;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Serine/arginine-rich splicing factor SR34A;
DE Short=At-SR34A;
DE Short=At-SRp34A;
DE Short=AtSR34A;
DE AltName: Full=SER/ARG-rich protein 34A;
GN Name=SR34A; Synonyms=SRP34A; OrderedLocusNames=At3g49430;
GN ORFNames=T1G12.13, T9C5.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Kim C.J., Bautista V.R., Chen H., De Los Reyes C., Wu S.Y., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP ALTERNATIVE SPLICING, AND INDUCTION.
RX PubMed=17319848; DOI=10.1111/j.1365-313x.2006.03020.x;
RA Palusa S.G., Ali G.S., Reddy A.S.;
RT "Alternative splicing of pre-mRNAs of Arabidopsis serine/arginine-rich
RT proteins: regulation by hormones and stresses.";
RL Plant J. 49:1091-1107(2007).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=20884799; DOI=10.1105/tpc.110.078352;
RA Barta A., Kalyna M., Reddy A.S.;
RT "Implementing a rational and consistent nomenclature for serine/arginine-
RT rich protein splicing factors (SR proteins) in plants.";
RL Plant Cell 22:2926-2929(2010).
RN [6]
RP GENE FAMILY.
RX PubMed=21935421; DOI=10.1371/journal.pone.0024542;
RA Richardson D.N., Rogers M.F., Labadorf A., Ben-Hur A., Guo H.,
RA Paterson A.H., Reddy A.S.N.;
RT "Comparative analysis of serine/arginine-rich proteins across 27
RT eukaryotes: insights into sub-family classification and extent of
RT alternative splicing.";
RL PLoS ONE 6:E24542-E24542(2011).
CC -!- FUNCTION: Probably involved in intron recognition and spliceosome
CC assembly.
CC -!- SUBUNIT: Component of the spliceosome.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250|UniProtKB:O22315}.
CC Nucleus, nucleoplasm {ECO:0000250|UniProtKB:O22315}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A2RVS6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2RVS6-2; Sequence=VSP_054990;
CC -!- MISCELLANEOUS: The splicing pattern of the pre-mRNA is regulated in a
CC tissue-specific manner and by development, and changes in response to
CC various types of abiotic stresses and hormones.
CC {ECO:0000305|PubMed:17319848}.
CC -!- SIMILARITY: Belongs to the splicing factor SR family. SR subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52185.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB62448.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC012329; AAG52185.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL132964; CAB62448.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78541.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78542.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78543.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63803.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63804.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63805.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63806.1; -; Genomic_DNA.
DR EMBL; BT030067; ABN04805.1; -; mRNA.
DR PIR; T46221; T46221.
DR RefSeq; NP_001078264.1; NM_001084795.1. [A2RVS6-2]
DR RefSeq; NP_001190041.1; NM_001203112.1. [A2RVS6-1]
DR RefSeq; NP_001325874.1; NM_001339418.1. [A2RVS6-1]
DR RefSeq; NP_001325875.1; NM_001339417.1. [A2RVS6-1]
DR RefSeq; NP_001325876.1; NM_001339419.1. [A2RVS6-1]
DR RefSeq; NP_001325877.1; NM_001339420.1. [A2RVS6-1]
DR RefSeq; NP_190512.3; NM_114803.6. [A2RVS6-1]
DR AlphaFoldDB; A2RVS6; -.
DR SMR; A2RVS6; -.
DR BioGRID; 9423; 9.
DR IntAct; A2RVS6; 1.
DR STRING; 3702.AT3G49430.3; -.
DR iPTMnet; A2RVS6; -.
DR MetOSite; A2RVS6; -.
DR PaxDb; A2RVS6; -.
DR PRIDE; A2RVS6; -.
DR ProteomicsDB; 226708; -. [A2RVS6-1]
DR EnsemblPlants; AT3G49430.1; AT3G49430.1; AT3G49430. [A2RVS6-1]
DR EnsemblPlants; AT3G49430.2; AT3G49430.2; AT3G49430. [A2RVS6-2]
DR EnsemblPlants; AT3G49430.3; AT3G49430.3; AT3G49430. [A2RVS6-1]
DR EnsemblPlants; AT3G49430.4; AT3G49430.4; AT3G49430. [A2RVS6-1]
DR EnsemblPlants; AT3G49430.5; AT3G49430.5; AT3G49430. [A2RVS6-1]
DR EnsemblPlants; AT3G49430.6; AT3G49430.6; AT3G49430. [A2RVS6-1]
DR EnsemblPlants; AT3G49430.7; AT3G49430.7; AT3G49430. [A2RVS6-1]
DR GeneID; 824105; -.
DR Gramene; AT3G49430.1; AT3G49430.1; AT3G49430. [A2RVS6-1]
DR Gramene; AT3G49430.2; AT3G49430.2; AT3G49430. [A2RVS6-2]
DR Gramene; AT3G49430.3; AT3G49430.3; AT3G49430. [A2RVS6-1]
DR Gramene; AT3G49430.4; AT3G49430.4; AT3G49430. [A2RVS6-1]
DR Gramene; AT3G49430.5; AT3G49430.5; AT3G49430. [A2RVS6-1]
DR Gramene; AT3G49430.6; AT3G49430.6; AT3G49430. [A2RVS6-1]
DR Gramene; AT3G49430.7; AT3G49430.7; AT3G49430. [A2RVS6-1]
DR KEGG; ath:AT3G49430; -.
DR Araport; AT3G49430; -.
DR TAIR; locus:2114643; AT3G49430.
DR eggNOG; KOG0105; Eukaryota.
DR InParanoid; A2RVS6; -.
DR OMA; HEDCSIG; -.
DR OrthoDB; 1321443at2759; -.
DR PhylomeDB; A2RVS6; -.
DR PRO; PR:A2RVS6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; A2RVS6; baseline and differential.
DR Genevisible; A2RVS6; AT.
DR GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0008380; P:RNA splicing; NAS:TAIR.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; mRNA processing; mRNA splicing; Nucleus;
KW Phosphoprotein; Reference proteome; Repeat; RNA-binding; Spliceosome.
FT CHAIN 1..300
FT /note="Serine/arginine-rich splicing factor SR34A"
FT /id="PRO_0000429596"
FT DOMAIN 7..82
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 122..200
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 81..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 198..300
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 207..235
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 273..289
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 207
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P92964"
FT MOD_RES 209
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P92964"
FT MOD_RES 231
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P92964"
FT MOD_RES 233
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9FYB7"
FT MOD_RES 239
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P92966"
FT MOD_RES 259
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9SJA6"
FT MOD_RES 275
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P92965"
FT MOD_RES 285
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9FYB7"
FT VAR_SEQ 273..300
FT /note="AMSRSKSRSRSRSRSPSKSPPKVREGSV -> QVFCPSFVFFLSTIMILLFV
FT LLFLL (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_054990"
SQ SEQUENCE 300 AA; 33599 MW; 38AE7C228F825776 CRC64;
MSGRFSRSIY VGNLPGDIRE HEIEDIFYKY GRIVDIELKV PPRPPCYCFV EFEHSRDAED
AIKGRDGYNL DGCRLRVELA HGGRGQSSSD RRGGYGGGGS GYGGGGGGGG SARFGVSRHS
EFRVIVRGLP SSASWQDLKD HMRKAGDVCF AEVTRDSDGT YGVVDYTNYD DMKYAIRKLD
DTEFRNPWAR GFIRVKKYES SRSRSRSPSR SRSRSRSRSR SRGRGRSHSR SRSLSRSKSP
RKDLSKSPRR SLSRSISKSR SPSPDKKKSP PRAMSRSKSR SRSRSRSPSK SPPKVREGSV
