SR541_ARATH
ID SR541_ARATH Reviewed; 479 AA.
AC P37106;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Signal recognition particle 54 kDa protein 1;
DE Short=SRP54;
GN Name=SRP-54A; Synonyms=SRP-54; OrderedLocusNames=At1g15310;
GN ORFNames=F9L1.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=8292790; DOI=10.1007/bf00042359;
RA Lindstrom J.T., Chu B., Belanger F.C.;
RT "Isolation and characterization of an Arabidopsis thaliana gene for the 54
RT kDa subunit of the signal recognition particle.";
RL Plant Mol. Biol. 23:1265-1272(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Binds to the signal sequence of presecretory protein when
CC they emerge from the ribosomes and transfers them to TRAM
CC (translocating chain-associating membrane protein).
CC -!- SUBUNIT: Signal recognition particle consists of a 7S RNA molecule of
CC 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19,
CC SRP14 and SRP9.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- DOMAIN: Has a two domain structure: the G-domain binds GTP; the M-
CC domain binds the 7S RNA in presence of SRP19 and also binds the signal
CC sequence.
CC -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily.
CC {ECO:0000305}.
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DR EMBL; L19997; AAA19728.1; -; Unassigned_DNA.
DR EMBL; AC007591; AAD39659.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29301.1; -; Genomic_DNA.
DR EMBL; AF344329; AAK06880.1; -; mRNA.
DR EMBL; AY086187; AAM64266.1; -; mRNA.
DR PIR; S42550; S42550.
DR RefSeq; NP_563970.1; NM_101400.4.
DR AlphaFoldDB; P37106; -.
DR SMR; P37106; -.
DR BioGRID; 23340; 1.
DR DIP; DIP-59002N; -.
DR IntAct; P37106; 2.
DR MINT; P37106; -.
DR STRING; 3702.AT1G15310.1; -.
DR PaxDb; P37106; -.
DR PRIDE; P37106; -.
DR ProteomicsDB; 226915; -.
DR EnsemblPlants; AT1G15310.1; AT1G15310.1; AT1G15310.
DR GeneID; 838100; -.
DR Gramene; AT1G15310.1; AT1G15310.1; AT1G15310.
DR KEGG; ath:AT1G15310; -.
DR Araport; AT1G15310; -.
DR TAIR; locus:2037803; AT1G15310.
DR eggNOG; KOG0780; Eukaryota.
DR HOGENOM; CLU_009301_6_1_1; -.
DR InParanoid; P37106; -.
DR OMA; LRIMHEQ; -.
DR OrthoDB; 463152at2759; -.
DR PhylomeDB; P37106; -.
DR PRO; PR:P37106; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P37106; baseline and differential.
DR Genevisible; P37106; AT.
DR GO; GO:0005737; C:cytoplasm; ISS:TAIR.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; ISS:TAIR.
DR GO; GO:0008312; F:7S RNA binding; IBA:GO_Central.
DR GO; GO:0030942; F:endoplasmic reticulum signal peptide binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IBA:GO_Central.
DR Gene3D; 1.10.260.30; -; 1.
DR Gene3D; 1.20.120.140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00306; SRP54; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036891; Signal_recog_part_SRP54_M_sf.
DR InterPro; IPR013822; Signal_recog_particl_SRP54_hlx.
DR InterPro; IPR004125; Signal_recog_particle_SRP54_M.
DR InterPro; IPR036225; SRP/SRP_N.
DR InterPro; IPR022941; SRP54.
DR InterPro; IPR006325; SRP54_euk.
DR InterPro; IPR000897; SRP54_GTPase_dom.
DR InterPro; IPR042101; SRP54_N_sf.
DR PANTHER; PTHR11564; PTHR11564; 1.
DR Pfam; PF00448; SRP54; 1.
DR Pfam; PF02881; SRP54_N; 1.
DR Pfam; PF02978; SRP_SPB; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00962; SRP54; 1.
DR SMART; SM00963; SRP54_N; 1.
DR SUPFAM; SSF47364; SSF47364; 1.
DR SUPFAM; SSF47446; SSF47446; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01425; SRP54_euk; 1.
DR PROSITE; PS00300; SRP54; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; GTP-binding; Nucleotide-binding; Reference proteome;
KW Ribonucleoprotein; RNA-binding; Signal recognition particle.
FT CHAIN 1..479
FT /note="Signal recognition particle 54 kDa protein 1"
FT /id="PRO_0000101204"
FT REGION 1..295
FT /note="G-domain"
FT REGION 296..479
FT /note="M-domain"
FT BINDING 108..115
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 190..194
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 248..251
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 479 AA; 53007 MW; DA4C0634420F486E CRC64;
MVLAELGGRI TRAIQQMNNV TIIDEKVLND FLNEITRALL QSDVSFGLVE KMQTNIKKIV
NLDDLAAGHN KRLIIEQAIF KELCRMLDPG KPAFAPKKAK PSVVMFVGLQ GAGKTTTCTK
YAYYHQKKGY KAALVCADTF RAGAFDQLKQ NATKAKIPFY GSYTESDPVK IAVEGVDRFK
KEKCDLIIVD TSGRHKQAAS LFEEMRQVAE ATEPDLVIFV MDSSIGQAAF EQAEAFKETV
SVGAVIITKM DGHAKGGGAL SAVAATKSPV IFIGTGEHMD EFEVFDVKPF VSRLLGKGDW
SGLVDKLQEV VPKDLQNELV ENLSQGNFTL RSMYDQFQCS LRIPLNQLFS MLPGISAEMM
PKGHGEESRV KMKRYMTMMD SMTNKELDSP NPKIFNESRI MRIARGSGRL VREVMEMLEE
YKRIAKTMKG IKIPKNGDMS KVIPPQMLKQ MGGMSGLQSL MKQMGSAKDM MGMFGGGGK
