SR541_SOLLC
ID SR541_SOLLC Reviewed; 496 AA.
AC P49971;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Signal recognition particle 54 kDa protein 1;
DE Short=SRP54;
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Rentita;
RX PubMed=7808407; DOI=10.1007/bf00282219;
RA Krolkiewicz S., Sanger H.L., Niesbach-Klosgen U.;
RT "Structural and functional characterisation of the signal recognition
RT particle-specific 54 kDa protein (SRP54) of tomato.";
RL Mol. Gen. Genet. 245:565-576(1994).
CC -!- FUNCTION: Binds to the signal sequence of presecretory protein when
CC they emerge from the ribosomes and transfers them to TRAM
CC (translocating chain-associating membrane protein).
CC -!- SUBUNIT: Signal recognition particle consists of a 7S RNA molecule of
CC 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19,
CC SRP14 and SRP9.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- DOMAIN: Has a two domain structure: the G-domain binds GTP; the M-
CC domain binds the 7S RNA in presence of SRP19 and also binds the signal
CC sequence.
CC -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily.
CC {ECO:0000305}.
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DR EMBL; Z34518; CAA84275.1; -; Genomic_DNA.
DR PIR; S51597; S51597.
DR AlphaFoldDB; P49971; -.
DR SMR; P49971; -.
DR STRING; 4081.Solyc03g116810.2.1; -.
DR PaxDb; P49971; -.
DR PRIDE; P49971; -.
DR eggNOG; KOG0780; Eukaryota.
DR Proteomes; UP000004994; Unplaced.
DR ExpressionAtlas; P49971; baseline and differential.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IBA:GO_Central.
DR GO; GO:0008312; F:7S RNA binding; IBA:GO_Central.
DR GO; GO:0030942; F:endoplasmic reticulum signal peptide binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IBA:GO_Central.
DR Gene3D; 1.10.260.30; -; 1.
DR Gene3D; 1.20.120.140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00306; SRP54; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036891; Signal_recog_part_SRP54_M_sf.
DR InterPro; IPR013822; Signal_recog_particl_SRP54_hlx.
DR InterPro; IPR004125; Signal_recog_particle_SRP54_M.
DR InterPro; IPR036225; SRP/SRP_N.
DR InterPro; IPR022941; SRP54.
DR InterPro; IPR006325; SRP54_euk.
DR InterPro; IPR000897; SRP54_GTPase_dom.
DR InterPro; IPR042101; SRP54_N_sf.
DR PANTHER; PTHR11564; PTHR11564; 1.
DR Pfam; PF00448; SRP54; 1.
DR Pfam; PF02881; SRP54_N; 1.
DR Pfam; PF02978; SRP_SPB; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00962; SRP54; 1.
DR SMART; SM00963; SRP54_N; 1.
DR SUPFAM; SSF47364; SSF47364; 1.
DR SUPFAM; SSF47446; SSF47446; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01425; SRP54_euk; 1.
DR PROSITE; PS00300; SRP54; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTP-binding; Nucleotide-binding; Reference proteome;
KW Ribonucleoprotein; RNA-binding; Signal recognition particle.
FT CHAIN 1..496
FT /note="Signal recognition particle 54 kDa protein 1"
FT /id="PRO_0000101210"
FT REGION 1..296
FT /note="G-domain"
FT REGION 297..496
FT /note="M-domain"
FT BINDING 108..115
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 191..195
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 249..252
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 496 AA; 54826 MW; 28075F27F80C7F5D CRC64;
MVLAQLGGSI SRALQQMSNA TIIDEKVLNE CLNEITRALL QADVQFKLVR DMSTNIKKIV
NLEDLAAGHN KRRIIQQAVY NELCKILDPG KPAFTLKKGK PSVVMFVGLQ GSGKTTTCTK
YAYHHQKRGW KPALVCADTF RAGAFDQLKQ NATKAKIPFY GSSYTESDPV KIAVDGVETF
KKENCDLIIV DTSGRHKQEA ALFEEMRQVS EAQKPDLVIF VMDSSIGQAA FDQAQAFRQS
VAVGAVIVTK MDGHAKGGGA LSRVAATKSP VIFIGTGEHM DEFEVFDVKP FVSRLLGMGD
LSGLVNKIQD VVPMDQQPEL LQKLSEGHFT LRIMYEQFQS MLKMGPLGVF SMLPGFSAEM
MPQGREKESQ AKFKRYMTMM DSMTDEELDS TNPKILTESR IMRIARGSGR LVHEVMEMLE
EYKRLAKIFS KMKGLKIPKK GDMSSLSRNM NAQNMSKVLP PQMLKQIGGM GGLQNLMKQM
GSAKDMMGMF GGGGGE