ID   SR541_SOLLC             Reviewed;         496 AA.
AC   P49971;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Signal recognition particle 54 kDa protein 1;
DE            Short=SRP54;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Rentita;
RX   PubMed=7808407; DOI=10.1007/bf00282219;
RA   Krolkiewicz S., Sanger H.L., Niesbach-Klosgen U.;
RT   "Structural and functional characterisation of the signal recognition
RT   particle-specific 54 kDa protein (SRP54) of tomato.";
RL   Mol. Gen. Genet. 245:565-576(1994).
CC   -!- FUNCTION: Binds to the signal sequence of presecretory protein when
CC       they emerge from the ribosomes and transfers them to TRAM
CC       (translocating chain-associating membrane protein).
CC   -!- SUBUNIT: Signal recognition particle consists of a 7S RNA molecule of
CC       300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19,
CC       SRP14 and SRP9.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DOMAIN: Has a two domain structure: the G-domain binds GTP; the M-
CC       domain binds the 7S RNA in presence of SRP19 and also binds the signal
CC       sequence.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; Z34518; CAA84275.1; -; Genomic_DNA.
DR   PIR; S51597; S51597.
DR   AlphaFoldDB; P49971; -.
DR   SMR; P49971; -.
DR   STRING; 4081.Solyc03g116810.2.1; -.
DR   PaxDb; P49971; -.
DR   PRIDE; P49971; -.
DR   eggNOG; KOG0780; Eukaryota.
DR   Proteomes; UP000004994; Unplaced.
DR   ExpressionAtlas; P49971; baseline and differential.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IBA:GO_Central.
DR   GO; GO:0008312; F:7S RNA binding; IBA:GO_Central.
DR   GO; GO:0030942; F:endoplasmic reticulum signal peptide binding; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IBA:GO_Central.
DR   Gene3D; 1.10.260.30; -; 1.
DR   Gene3D; 1.20.120.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00306; SRP54; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036891; Signal_recog_part_SRP54_M_sf.
DR   InterPro; IPR013822; Signal_recog_particl_SRP54_hlx.
DR   InterPro; IPR004125; Signal_recog_particle_SRP54_M.
DR   InterPro; IPR036225; SRP/SRP_N.
DR   InterPro; IPR022941; SRP54.
DR   InterPro; IPR006325; SRP54_euk.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   InterPro; IPR042101; SRP54_N_sf.
DR   PANTHER; PTHR11564; PTHR11564; 1.
DR   Pfam; PF00448; SRP54; 1.
DR   Pfam; PF02881; SRP54_N; 1.
DR   Pfam; PF02978; SRP_SPB; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SMART; SM00963; SRP54_N; 1.
DR   SUPFAM; SSF47364; SSF47364; 1.
DR   SUPFAM; SSF47446; SSF47446; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01425; SRP54_euk; 1.
DR   PROSITE; PS00300; SRP54; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; GTP-binding; Nucleotide-binding; Reference proteome;
KW   Ribonucleoprotein; RNA-binding; Signal recognition particle.
FT   CHAIN           1..496
FT                   /note="Signal recognition particle 54 kDa protein 1"
FT                   /id="PRO_0000101210"
FT   REGION          1..296
FT                   /note="G-domain"
FT   REGION          297..496
FT                   /note="M-domain"
FT   BINDING         108..115
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         191..195
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         249..252
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   496 AA;  54826 MW;  28075F27F80C7F5D CRC64;
     MVLAQLGGSI SRALQQMSNA TIIDEKVLNE CLNEITRALL QADVQFKLVR DMSTNIKKIV
     NLEDLAAGHN KRRIIQQAVY NELCKILDPG KPAFTLKKGK PSVVMFVGLQ GSGKTTTCTK
     YAYHHQKRGW KPALVCADTF RAGAFDQLKQ NATKAKIPFY GSSYTESDPV KIAVDGVETF
     KKENCDLIIV DTSGRHKQEA ALFEEMRQVS EAQKPDLVIF VMDSSIGQAA FDQAQAFRQS
     VAVGAVIVTK MDGHAKGGGA LSRVAATKSP VIFIGTGEHM DEFEVFDVKP FVSRLLGMGD
     LSGLVNKIQD VVPMDQQPEL LQKLSEGHFT LRIMYEQFQS MLKMGPLGVF SMLPGFSAEM
     MPQGREKESQ AKFKRYMTMM DSMTDEELDS TNPKILTESR IMRIARGSGR LVHEVMEMLE
     EYKRLAKIFS KMKGLKIPKK GDMSSLSRNM NAQNMSKVLP PQMLKQIGGM GGLQNLMKQM
     GSAKDMMGMF GGGGGE