SR542_ARATH
ID SR542_ARATH Reviewed; 497 AA.
AC P49966;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Signal recognition particle 54 kDa protein 2;
DE Short=SRP54;
GN Name=SRP-54B; OrderedLocusNames=At5g49500; ORFNames=K6M13.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7824644; DOI=10.1104/pp.106.3.1157;
RA Chu B., Lindstrom J.T., Belanger F.C.;
RT "Arabidopsis thaliana expresses three divergent Srp54 genes.";
RL Plant Physiol. 106:1157-1162(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Binds to the signal sequence of presecretory protein when
CC they emerge from the ribosomes and transfers them to TRAM
CC (translocating chain-associating membrane protein).
CC -!- SUBUNIT: Signal recognition particle consists of a 7S RNA molecule of
CC 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19,
CC SRP14 and SRP9.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- DOMAIN: Has a two domain structure: the G-domain binds GTP; the M-
CC domain binds the 7S RNA in presence of SRP19 and also binds the signal
CC sequence.
CC -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA66199.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAA66199.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; U12126; AAA66199.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AB023033; BAB10763.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95821.1; -; Genomic_DNA.
DR RefSeq; NP_199761.1; NM_124327.2.
DR AlphaFoldDB; P49966; -.
DR SMR; P49966; -.
DR BioGRID; 20256; 2.
DR IntAct; P49966; 1.
DR STRING; 3702.AT5G49500.1; -.
DR PaxDb; P49966; -.
DR PRIDE; P49966; -.
DR ProteomicsDB; 226719; -.
DR EnsemblPlants; AT5G49500.1; AT5G49500.1; AT5G49500.
DR GeneID; 835010; -.
DR Gramene; AT5G49500.1; AT5G49500.1; AT5G49500.
DR KEGG; ath:AT5G49500; -.
DR Araport; AT5G49500; -.
DR TAIR; locus:2157809; AT5G49500.
DR eggNOG; KOG0780; Eukaryota.
DR HOGENOM; CLU_009301_6_1_1; -.
DR InParanoid; P49966; -.
DR OMA; RFEKENC; -.
DR OrthoDB; 463152at2759; -.
DR PRO; PR:P49966; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P49966; baseline and differential.
DR Genevisible; P49966; AT.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IBA:GO_Central.
DR GO; GO:0008312; F:7S RNA binding; IBA:GO_Central.
DR GO; GO:0030942; F:endoplasmic reticulum signal peptide binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IBA:GO_Central.
DR Gene3D; 1.10.260.30; -; 1.
DR Gene3D; 1.20.120.140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00306; SRP54; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036891; Signal_recog_part_SRP54_M_sf.
DR InterPro; IPR013822; Signal_recog_particl_SRP54_hlx.
DR InterPro; IPR004125; Signal_recog_particle_SRP54_M.
DR InterPro; IPR036225; SRP/SRP_N.
DR InterPro; IPR022941; SRP54.
DR InterPro; IPR006325; SRP54_euk.
DR InterPro; IPR000897; SRP54_GTPase_dom.
DR InterPro; IPR042101; SRP54_N_sf.
DR PANTHER; PTHR11564; PTHR11564; 1.
DR Pfam; PF00448; SRP54; 1.
DR Pfam; PF02881; SRP54_N; 1.
DR Pfam; PF02978; SRP_SPB; 1.
DR SMART; SM00962; SRP54; 1.
DR SMART; SM00963; SRP54_N; 1.
DR SUPFAM; SSF47364; SSF47364; 1.
DR SUPFAM; SSF47446; SSF47446; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01425; SRP54_euk; 1.
DR PROSITE; PS00300; SRP54; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTP-binding; Nucleotide-binding; Reference proteome;
KW Ribonucleoprotein; RNA-binding; Signal recognition particle.
FT CHAIN 1..497
FT /note="Signal recognition particle 54 kDa protein 2"
FT /id="PRO_0000101205"
FT REGION 1..297
FT /note="G-domain"
FT REGION 298..497
FT /note="M-domain"
FT BINDING 108..117
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 192..196
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 250..253
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 497 AA; 55263 MW; A84627610B7B75A2 CRC64;
MVLAELGGRI MSAIQKMNNV TIIDEKALND CLNEITRALL QSDVSFPLVK EMQTNIKKIV
NLEDLAAGHN KRRIIEQAIF SELCKMLDPG KSAFAPKKAK PSVVMFVGLQ GEVLEKPQLV
PSMLIIIRRK GYKPALVCAD TFRAGAFDQL KQNATKSKIP YYGSYTGSDP VKIAVEGVDR
FKKENCDLII VDTSGRHKQQ ASLFEEMRQI SEATKPDLVI FVMDSSIGQT AFEQARAFKQ
TVAVGAVIIT KMDGHAKGGG TLSAVAATKS PVIFIGTGEH MDEFEVFDAK PFVSRLLGNG
DMSGFVNKLQ EVVPKDQQPE LLEMLSHGKF TLRIMYDQFQ NMLNMGPLKE VFSMLPGMRA
EMMPEGHEKE SQAKIKRYMT MMDSMTNEEL DSSNPKVFNE SRIMRIARGS GRIVREVMEM
LEEYKRLTTM WGKVKGLKNL EKGNMSALSK NKNSQQLSKV LPAQMLKQIG GMSGLQSLMK
QMGSGKDLMG MFGGRDE
