ID   SR542_HORVU             Reviewed;         497 AA.
AC   P49969;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Signal recognition particle 54 kDa protein 2;
DE            Short=SRP54;
GN   Name=SRP54-2;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Root, and Shoot;
RA   Chu B., Brodl M.R., Belanger F.C.;
RL   Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Binds to the signal sequence of presecretory protein when
CC       they emerge from the ribosomes and transfers them to TRAM
CC       (translocating chain-associating membrane protein).
CC   -!- SUBUNIT: Signal recognition particle consists of a 7S RNA molecule of
CC       300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19,
CC       SRP14 and SRP9.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DOMAIN: Has a two domain structure: the G-domain binds GTP; the M-
CC       domain binds the 7S RNA in presence of SRP19 and also binds the signal
CC       sequence.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; L48285; AAA79355.1; -; mRNA.
DR   PIR; T06186; T06186.
DR   AlphaFoldDB; P49969; -.
DR   SMR; P49969; -.
DR   EnsemblPlants; HORVU.MOREX.r2.2HG0094240.1; HORVU.MOREX.r2.2HG0094240.1; HORVU.MOREX.r2.2HG0094240.
DR   EnsemblPlants; HORVU.MOREX.r2.2HG0094240.1.mrna1; HORVU.MOREX.r2.2HG0094240.1.mrna1; HORVU.MOREX.r2.2HG0094240.1.
DR   Gramene; HORVU.MOREX.r2.2HG0094240.1; HORVU.MOREX.r2.2HG0094240.1; HORVU.MOREX.r2.2HG0094240.
DR   Gramene; HORVU.MOREX.r2.2HG0094240.1.mrna1; HORVU.MOREX.r2.2HG0094240.1.mrna1; HORVU.MOREX.r2.2HG0094240.1.
DR   ExpressionAtlas; P49969; baseline and differential.
DR   GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IEA:UniProtKB-KW.
DR   GO; GO:0008312; F:7S RNA binding; IEA:InterPro.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR   Gene3D; 1.10.260.30; -; 1.
DR   Gene3D; 1.20.120.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00306; SRP54; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036891; Signal_recog_part_SRP54_M_sf.
DR   InterPro; IPR013822; Signal_recog_particl_SRP54_hlx.
DR   InterPro; IPR004125; Signal_recog_particle_SRP54_M.
DR   InterPro; IPR036225; SRP/SRP_N.
DR   InterPro; IPR022941; SRP54.
DR   InterPro; IPR006325; SRP54_euk.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   InterPro; IPR042101; SRP54_N_sf.
DR   PANTHER; PTHR11564; PTHR11564; 1.
DR   Pfam; PF00448; SRP54; 1.
DR   Pfam; PF02881; SRP54_N; 1.
DR   Pfam; PF02978; SRP_SPB; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SMART; SM00963; SRP54_N; 1.
DR   SUPFAM; SSF47364; SSF47364; 1.
DR   SUPFAM; SSF47446; SSF47446; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01425; SRP54_euk; 1.
DR   PROSITE; PS00300; SRP54; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; GTP-binding; Nucleotide-binding; Ribonucleoprotein; RNA-binding;
KW   Signal recognition particle.
FT   CHAIN           1..497
FT                   /note="Signal recognition particle 54 kDa protein 2"
FT                   /id="PRO_0000101208"
FT   REGION          1..295
FT                   /note="G-domain"
FT   REGION          296..497
FT                   /note="M-domain"
FT   BINDING         108..115
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         190..194
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         248..251
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   497 AA;  54508 MW;  151A2A4473B2EA3D CRC64;
     MVLAQLGGSI SRALAQMSNA TVIDEKVLGE CLNEISRALL QSDVQFKMVR DMQTNIRKIV
     NLETLAAGTN KRRIIQQAVF TELCNMLDPG KPAFTPKKGK PSVVMFVGLQ GSGKTTTCTK
     YAYYHQRKGF KPSLVCADTF RAGAFDQLKQ NATKAKIPFY GSYMESDPVK IAVEGLERFR
     KENSDLIIID TSGRHKQEAA LFEEMRQVAE ATKPDLVIFV MDGSIGQAAF DQAQAFKQSA
     SVGAVIITKL DGHAKGGGAL SAVAATKSPV IFIGTGEHID EFEIFDVKPF VSRLLGMGDL
     SGLMDKIQDV MPADQQPELL AKLAEGTFTL RLLYEQFQNL LKMGPIGQVF SMLPGFSSEL
     MPKGHEKEGQ AKIKRYMTIM DSMTAAELDS TNPKLMTESR IIRIARGSGR QIRDVTDMLE
     EYKRLAKMWS KMKGLKMPKN GKMSDLSQNL NIQQMTKALP PQVLKQMGGM GGLQALMKQM
     GGKDMSKMLG GMGLGGD