ID   SR542_SOLLC             Reviewed;         499 AA.
AC   P49972;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Signal recognition particle 54 kDa protein 2;
DE            Short=SRP54;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. UC82B;
RX   PubMed=7808407; DOI=10.1007/bf00282219;
RA   Krolkiewicz S., Sanger H.L., Niesbach-Klosgen U.;
RT   "Structural and functional characterisation of the signal recognition
RT   particle-specific 54 kDa protein (SRP54) of tomato.";
RL   Mol. Gen. Genet. 245:565-576(1994).
CC   -!- FUNCTION: Binds to the signal sequence of presecretory protein when
CC       they emerge from the ribosomes and transfers them to TRAM
CC       (translocating chain-associating membrane protein).
CC   -!- SUBUNIT: Signal recognition particle consists of a 7S RNA molecule of
CC       300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19,
CC       SRP14 and SRP9.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DOMAIN: Has a two domain structure: the G-domain binds GTP; the M-
CC       domain binds the 7S RNA in presence of SRP19 and also binds the signal
CC       sequence.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; Z34527; CAA84288.1; -; mRNA.
DR   PIR; S51598; S51598.
DR   RefSeq; NP_001234428.1; NM_001247499.2.
DR   AlphaFoldDB; P49972; -.
DR   SMR; P49972; -.
DR   STRING; 4081.Solyc12g042740.1.1; -.
DR   PaxDb; P49972; -.
DR   PRIDE; P49972; -.
DR   EnsemblPlants; Solyc12g042740.2.1; Solyc12g042740.2.1; Solyc12g042740.2.
DR   GeneID; 544156; -.
DR   Gramene; Solyc12g042740.2.1; Solyc12g042740.2.1; Solyc12g042740.2.
DR   KEGG; sly:544156; -.
DR   eggNOG; KOG0780; Eukaryota.
DR   HOGENOM; CLU_009301_6_1_1; -.
DR   InParanoid; P49972; -.
DR   OMA; SEVFSML; -.
DR   OrthoDB; 463152at2759; -.
DR   PhylomeDB; P49972; -.
DR   Proteomes; UP000004994; Chromosome 12.
DR   ExpressionAtlas; P49972; baseline and differential.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IBA:GO_Central.
DR   GO; GO:0008312; F:7S RNA binding; IBA:GO_Central.
DR   GO; GO:0030942; F:endoplasmic reticulum signal peptide binding; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IBA:GO_Central.
DR   Gene3D; 1.10.260.30; -; 1.
DR   Gene3D; 1.20.120.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00306; SRP54; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036891; Signal_recog_part_SRP54_M_sf.
DR   InterPro; IPR013822; Signal_recog_particl_SRP54_hlx.
DR   InterPro; IPR004125; Signal_recog_particle_SRP54_M.
DR   InterPro; IPR036225; SRP/SRP_N.
DR   InterPro; IPR022941; SRP54.
DR   InterPro; IPR006325; SRP54_euk.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   InterPro; IPR042101; SRP54_N_sf.
DR   PANTHER; PTHR11564; PTHR11564; 1.
DR   Pfam; PF00448; SRP54; 1.
DR   Pfam; PF02881; SRP54_N; 1.
DR   Pfam; PF02978; SRP_SPB; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SMART; SM00963; SRP54_N; 1.
DR   SUPFAM; SSF47364; SSF47364; 1.
DR   SUPFAM; SSF47446; SSF47446; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01425; SRP54_euk; 1.
DR   PROSITE; PS00300; SRP54; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; GTP-binding; Nucleotide-binding; Reference proteome;
KW   Ribonucleoprotein; RNA-binding; Signal recognition particle.
FT   CHAIN           1..499
FT                   /note="Signal recognition particle 54 kDa protein 2"
FT                   /id="PRO_0000101211"
FT   REGION          1..295
FT                   /note="G-domain"
FT   REGION          296..499
FT                   /note="M-domain"
FT   BINDING         108..115
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         190..194
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         248..251
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   499 AA;  55184 MW;  1316B7288D007F6B CRC64;
     MVLAELGGSI SRALQQMSNA TIIDEKVLNE CLNEITRALL QADVQFKLVR DMTTNIKKIV
     NLDDLAAGHN KRRIIQQAVF NELCKILDPG KPSFTPKKGK PSIVMFVGLQ GSGKTTTCTK
     YAYYHQKKGW KPALVCADTF RAGAFDQLKQ NATKAKIPFY GSYTESDPVK IAVDGVETFK
     KENCDLIIVD TSGRHKQEAA LFEEMRQVAE ATKPDLVIFV MDSSIGQAAF DQAQAFKQSV
     AVGAVIVTKM DGHAKGGGAL SAVAATKSPV IFIGTGEHMD EFEVFDVKPF VSRLLGMGDW
     SGFMDKIHEV VPMDQQPELL QKLSEGHFTL RIMYEQFQNI LKMGPIGQVF SMLPGFSAEL
     MPKGRENESQ AKIKRYMTMM DSMTNEELDS SNPKLMTDSR IMRIARGSGR QVHEVMDMME
     EYKRLAKIWS KMKGLKIPKK GEMSALSRNM NAQHMSKVLP PQMLKQIGGM GGLQNLMKQM
     GSAKDMMGGM GGMFGGGDK