SR543_ARATH
ID SR543_ARATH Reviewed; 495 AA.
AC P49967; Q93W49; Q9FVP9;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Signal recognition particle 54 kDa protein 3;
DE Short=SRP54;
GN Name=SRP-54C; OrderedLocusNames=At1g48900; ORFNames=F27K7.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7824644; DOI=10.1104/pp.106.3.1157;
RA Chu B., Lindstrom J.T., Belanger F.C.;
RT "Arabidopsis thaliana expresses three divergent Srp54 genes.";
RL Plant Physiol. 106:1157-1162(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Binds to the signal sequence of presecretory protein when
CC they emerge from the ribosomes and transfers them to TRAM
CC (translocating chain-associating membrane protein).
CC -!- SUBUNIT: Signal recognition particle consists of a 7S RNA molecule of
CC 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19,
CC SRP14 and SRP9.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=P49967-1; Sequence=Displayed;
CC -!- DOMAIN: Has a two domain structure: the G-domain binds GTP; the M-
CC domain binds the 7S RNA in presence of SRP19 and also binds the signal
CC sequence.
CC -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG29734.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U12127; AAA66200.1; -; Genomic_DNA.
DR EMBL; AC084414; AAG29734.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32363.1; -; Genomic_DNA.
DR EMBL; AY054273; AAL06932.1; -; mRNA.
DR EMBL; AY052331; AAK96524.1; -; mRNA.
DR EMBL; AF446864; AAL38597.1; -; mRNA.
DR PIR; G96526; G96526.
DR RefSeq; NP_564535.1; NM_103783.2. [P49967-1]
DR AlphaFoldDB; P49967; -.
DR SMR; P49967; -.
DR BioGRID; 26537; 2.
DR STRING; 3702.AT1G48900.1; -.
DR iPTMnet; P49967; -.
DR PaxDb; P49967; -.
DR PRIDE; P49967; -.
DR ProteomicsDB; 226861; -. [P49967-1]
DR EnsemblPlants; AT1G48900.1; AT1G48900.1; AT1G48900. [P49967-1]
DR GeneID; 841312; -.
DR Gramene; AT1G48900.1; AT1G48900.1; AT1G48900. [P49967-1]
DR KEGG; ath:AT1G48900; -.
DR Araport; AT1G48900; -.
DR TAIR; locus:2028563; AT1G48900.
DR eggNOG; KOG0780; Eukaryota.
DR HOGENOM; CLU_009301_6_1_1; -.
DR InParanoid; P49967; -.
DR PhylomeDB; P49967; -.
DR PRO; PR:P49967; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P49967; baseline and differential.
DR Genevisible; P49967; AT.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IBA:GO_Central.
DR GO; GO:0008312; F:7S RNA binding; IBA:GO_Central.
DR GO; GO:0030942; F:endoplasmic reticulum signal peptide binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IBA:GO_Central.
DR Gene3D; 1.10.260.30; -; 1.
DR Gene3D; 1.20.120.140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00306; SRP54; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036891; Signal_recog_part_SRP54_M_sf.
DR InterPro; IPR013822; Signal_recog_particl_SRP54_hlx.
DR InterPro; IPR004125; Signal_recog_particle_SRP54_M.
DR InterPro; IPR036225; SRP/SRP_N.
DR InterPro; IPR022941; SRP54.
DR InterPro; IPR006325; SRP54_euk.
DR InterPro; IPR000897; SRP54_GTPase_dom.
DR InterPro; IPR042101; SRP54_N_sf.
DR PANTHER; PTHR11564; PTHR11564; 1.
DR Pfam; PF00448; SRP54; 1.
DR Pfam; PF02881; SRP54_N; 1.
DR Pfam; PF02978; SRP_SPB; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00962; SRP54; 1.
DR SMART; SM00963; SRP54_N; 1.
DR SUPFAM; SSF47364; SSF47364; 1.
DR SUPFAM; SSF47446; SSF47446; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01425; SRP54_euk; 1.
DR PROSITE; PS00300; SRP54; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; GTP-binding; Nucleotide-binding;
KW Reference proteome; Ribonucleoprotein; RNA-binding;
KW Signal recognition particle.
FT CHAIN 1..495
FT /note="Signal recognition particle 54 kDa protein 3"
FT /id="PRO_0000101206"
FT REGION 1..295
FT /note="G-domain"
FT REGION 296..495
FT /note="M-domain"
FT BINDING 108..115
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 190..194
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 248..251
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT CONFLICT 9
FT /note="R -> G (in Ref. 1; AAA66200)"
FT /evidence="ECO:0000305"
FT CONFLICT 99
FT /note="A -> R (in Ref. 1; AAA66200)"
FT /evidence="ECO:0000305"
FT CONFLICT 392
FT /note="N -> G (in Ref. 1; AAA66200)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 495 AA; 54782 MW; 5B03EE98CE8483BF CRC64;
MVLAELGGRI TRAIQQMSNV TIIDEKALNE CLNEITRALL QSDVSFPLVK EMQSNIKKIV
NLEDLAAGHN KRRIIEQAIF SELCKMLDPG KPAFAPKKAK ASVVMFVGLQ GAGKTTTCTK
YAYYHQKKGY KPALVCADTF RAGAFDQLKQ NATKAKIPFY GSYTESDPVK IAVEGVDTFK
KENCDLIIVD TSGRHKQEAS LFEEMRQVAE ATKPDLVIFV MDSSIGQAAF DQAQAFKQSV
AVGAVIITKM DGHAKGGGAL SAVAATKSPV IFIGTGEHMD EFEVFDVKPF VSRLLGMGDW
SGFVDKLQEV VPKDQQPELL EKLSQGNFTL RIMYDQFQNI LNMGPLKEVF SMLPGISAEM
MPKGHEKESQ AKIKRYMTMM DSMTNDELDS SNPKVFNESR MMRIARGSGR QVREVMEMLE
EYKRLAKIWS KMKGLKIPKN GDMSALSRNM NAQHMSKVLP PQMLKQIGGM GGLQSLMKQM
GSGKDMMGMF GGGDK
