ID   SRAP_STAHJ              Reviewed;        3608 AA.
AC   Q4L9P0;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   25-MAY-2022, entry version 121.
DE   RecName: Full=Serine-rich adhesin for platelets;
DE   AltName: Full=Adhesin SraP {ECO:0000305};
DE   Flags: Precursor;
GN   Name=sraP; OrderedLocusNames=SH0326;
OS   Staphylococcus haemolyticus (strain JCSC1435).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=279808;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCSC1435;
RX   PubMed=16237012; DOI=10.1128/jb.187.21.7292-7308.2005;
RA   Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y.,
RA   Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C.,
RA   Hiramatsu K.;
RT   "Whole-genome sequencing of Staphylococcus haemolyticus uncovers the
RT   extreme plasticity of its genome and the evolution of human-colonizing
RT   staphylococcal species.";
RL   J. Bacteriol. 187:7292-7308(2005).
CC   -!- FUNCTION: Mediates binding to human platelets, possibly through a
CC       receptor-ligand interaction. {ECO:0000250,
CC       ECO:0000250|UniProtKB:Q2FUW1}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC       ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC       ProRule:PRU00477}. Note=Exported by the accessory SecA2/SecY2 system.
CC       Anchored to the cell wall by sortase A (By similarity).
CC       {ECO:0000250|UniProtKB:Q2FUW1}.
CC   -!- PTM: Proteolytically cleaved by a metalloprotease. {ECO:0000250,
CC       ECO:0000250|UniProtKB:Q2FUW1}.
CC   -!- PTM: Glycosylated (By similarity). It is probable that most of the Ser
CC       residues in SSR1 and SSR2 are O-GlcNAcylated. Sequential glycosylation
CC       by sugar transferases are able to generate complex sugar polymorphisms
CC       (By similarity). {ECO:0000250|UniProtKB:A0A0H2URK1,
CC       ECO:0000250|UniProtKB:Q2FUW1}.
CC   -!- SIMILARITY: Belongs to the serine-rich repeat protein (SRRP) family.
CC       {ECO:0000305}.
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DR   EMBL; AP006716; BAE03635.1; -; Genomic_DNA.
DR   SMR; Q4L9P0; -.
DR   STRING; 279808.SH0326; -.
DR   EnsemblBacteria; BAE03635; BAE03635; SH0326.
DR   KEGG; sha:SH0326; -.
DR   eggNOG; COG3391; Bacteria.
DR   HOGENOM; CLU_224803_0_0_9; -.
DR   OMA; KTWSVNV; -.
DR   Proteomes; UP000000543; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR   InterPro; IPR022263; KxYKxGKxW.
DR   InterPro; IPR019931; LPXTG_anchor.
DR   Pfam; PF00746; Gram_pos_anchor; 1.
DR   Pfam; PF19258; KxYKxGKxW_sig; 1.
DR   TIGRFAMs; TIGR03715; KxYKxGKxW; 1.
DR   PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE   3: Inferred from homology;
KW   Cell adhesion; Cell wall; Glycoprotein; Peptidoglycan-anchor; Secreted;
KW   Signal; Virulence.
FT   SIGNAL          1..91
FT                   /evidence="ECO:0000250|UniProtKB:Q2FUW1"
FT   CHAIN           92..3567
FT                   /note="Serine-rich adhesin for platelets"
FT                   /id="PRO_0000273936"
FT   PROPEP          3568..3608
FT                   /note="Removed by sortase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT                   /id="PRO_0000273937"
FT   REGION          92..302
FT                   /note="Serine-rich repeat region 1, SRR1"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FUW1"
FT   REGION          105..280
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          303..755
FT                   /note="Non-repeat region (NRR)"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FUW1"
FT   REGION          495..3575
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          756..3567
FT                   /note="Serine-rich repeat region 2, SRR2"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FUW1, ECO:0000305"
FT   REGION          3588..3608
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           3564..3568
FT                   /note="LPXTG sorting signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT   COMPBIAS        495..3553
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        3554..3568
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         3567
FT                   /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
SQ   SEQUENCE   3608 AA;  344174 MW;  F6741519536D4ED7 CRC64;
     MSRKERNFKR FFGQEKARVK LYKSGKQWVK AGIREVQLLK VLGLPFLNKD VEQINNLDTN
     KDKNFKNQAM KATGLAGGAF TFAMLNDHHA YAASETPMTS EIASNSETVA NQNSTTVTKS
     ETSTTEYISS QTSTSQDATS STNSTEKSTS SSTTDSQTST DSTSDKSTSN SEKQDSSMSN
     SDTKASSSST TDNSTSNNST TSEKDTNSQA NTTSTDSQKG STSTNDNSIT STSTKDNQIR
     KNSTESNSIT ASNSTSDSNS GSTVSTNSTT SQLTSTSESQ INTDLGSTLL VSDSTSTSTS
     TAPLKLRTFS RLATTTFAAA AATSTTNTYT GAGTDTNYNI PIYYKLTTVN NGTSMTFTYT
     VTYDNPATTT VERPTALSNS YAIYNTGTTN QTMFTLGSAY GTPSTATSYI TDSTGAQVSN
     PRANTTNINK QGSGYTWANG YQMNGAQAKQ GYGLTTTWTV PINSSGDTSF TFNPYSTSVT
     GGTNFFNGKK VTVTDPTSTA NSQSASTSTA NSQSASTSKS TSTANSQSAS TSTSTSTANS
     QSASTSTSTS TANSQSASTS TSTSTANSQS ASTSTSTSTA NSQSTSTSTS TSTANSQSTS
     TSTSTSVSDS TSASTSLSGS TSTSVSDSTS ASTSLSDSAS TSVSDSTSAS TSLSASTSTS
     ESDSTSASTS LSESTSTSLS DSLSASTSLS DSASTSVSDS TSASTSLSGS ESASLSDSAS
     ASTSLSESTS TSESTSTSES DSTSASTSLS GSESASLSDS ASASTSLSGS ESASLSDSAS
     ASTSLSGSES ASLSDSASAS TSLSGSESAS LSDSASASTS LSGSESASLS DSASASTSLS
     ESTSTSLSDS ASASTSLSES TSTSVSDSTS ASTSLSASTS TSVSDSTSTS TSDSASTSTS
     VSDSTSTSTS LSGSTSTSVS DSTSASTSLS ASTSTSVSDS TSTSTSDSAS TSTSVSDSTS
     TSTSLSGSTS TSVSDSTSAS TSLSESTSTS LSDSASASTS LSESTSTSVS DSTSTSTSDS
     ASTSTSVSDS TSTSTSLSGS TSTSVSDSTS ASTSDSASTS TSVSDSTSAS TSDSASTSTS
     VSDSTSTSTS LSGSTSTSVS DSTSASTSLS ESTSTSVSDS TSASTSLSDS ASTSVSDSTS
     ASTSLSESTS TSVSDSTSTS TSLSESTSTS VSDSASASTS LSDSASTSVS DSTSASTSLS
     GSTSTSVSDS TSTSTSLSES TSTSLSDSAS ASTSLSDSAS TSVSDSTSAS TSLSGSTSTS
     VSDSTSTSTS LSESTSTSLS DSASASTSLS ASTSTSVSDS TSASTSLSGS TSTSESDSTS
     MSTSLSGSES TSLSDSLSAS TSLSGSTSTS VSDSTSASTS LSGSTSTSVS DSTSVSTSLS
     ASTSTSESDS TSTSTSDSAS TSTSVSDSTS ASTSLSASTS TSVSDSTSAS TSLSASTSTS
     VSDSTSASTS LSASTSTSVS DSTSASTSLS ASTSTSVSDS TSMSTSLSGS ESTSLSDSLS
     ASTSVSASTS TSVSDSTSAS TSLSGSTSTS VSDSTSTSTS LSESTSTSVS DSASASTSLS
     ASTSTSVSDS TSASTSLSAS TSTSVSDSTS ASTSLSASTS TSVSDSTSAS TSLSASTSTS
     VSDSTSTSTS LSASTSTSVS DSTSASTSLS GSASASLSDS LSASTSVSAS TSTSVSDSTS
     MSTSLSGSES TSLSDSLSAS TSVSASTSTS VSDSTSASTS LSGSTSTSVS DSTSTSTSLS
     ESTSTSVSDS ASASTSLSDS ASTSVSDSTS ASTSLSGSTS TSVSDSTSTS TSLSESTSTS
     LSDSASASTS LSDSASTSVS DSTSASTSLS GSTSTSVSDS TSTSTSLSES TSTSLSDSTS
     ISTSLSASTS TSESDSTSTS TSLSGSTSTS VSDSISRSTS LSGSTSTSVS DSTSTSTSDS
     ASTSTSVSDS TSASTSLSAS TSTSVSDSTS ASTSLSASTS TSVSDSTSAS TSLSASTSTS
     VSDSTSASTS LSASTSTSVS DSTSASTSLS ASTSTSVSDS TSASTSLSAS TSTSVSDSTS
     TSTSLSASTS TSVSDSTSAS TSLSGSASAS LSDSLSASTS VSASTSTSVS DSTSTSTSLS
     ESTSTSLSNS ASASTSLSGS TSTSVSDSTS ASTSLSASTS TSVSDSTSMS TSLSGSESTS
     LSDSLSASTS VSASTSTSVS DSTSASTSLS GSTSTSVSDS TSTSTSLSES TSTSVSDSAS
     ASTSLSDSAS TSVSDSTSAS TSLSGSTSTS VSDSTSTSTS LSESTSTSLS DSASASTSLS
     DSASTSVSDS TSASTSLSGS TSTSVSDSTS ASTSLSGSTS TSVSDSTSTS TSLSASTSTS
     ESDSTSTSTS LSGSTSTSVS DSISGSTSLS GSTSTSVSDS TSTSTSDSAS TSTSVSDSTS
     ASTSLSASTS TSVSDSTSAS TSLSASTSTS VSDSTSASTS LSGSASASLS DSLSASTSVS
     ASTSTSVSDS TSTSTSLSES TSTSLSNSAS ASTSLSGSTS TSVSDSTSAS TSLSASTSTS
     VSDSTSTSTS LSESTSTSLS DSASASTSLS DSASTSVSDS TSASTSLSGS ESTSLSDSAS
     ASTSLSASTS TSVSDSTSTS TSDSVSTSTS MSDSTSMSTS LSGSTSTSVS DSTSASTSLS
     GSTSTSVSDS TSVSTSLSAS TSTSESDSTS TSTSLSGSTS TSVSDSTSAS TSLSGSTSTS
     VSDSTSTSTS DSASTSTSVS DSTSASTSLS ASTSTSVSDS TSASTSLSAS TSTSVSDSTS
     ASTSLSASTS TSVSDSTSAS TSLSASTSTS VSDSTSTNTS LSASTSTSVS DSTSASTSLS
     ASTSTSVSDS TSASTSLSAS TSTSVSDSTS TSTSLSASTS TSVSDSTSAS TSLSGSASAS
     LSDSLSASTS VSASTSTSVS DSTSTSTSLS ESTSTSLSNS ASASTSLSGS ASASLSDSLS
     ASTSVSASTS TSVSDSTSTS TSLSESTSTS LSDSASASTS LSDSASTSVS DSTSASTSLS
     ESTSTSVSDS TSTSTSLSGS ESTSLSDSAS ASTSLSASTS TSVSDSTSTS TSDSVSTSTS
     MSDSTSMSTS LSGSTSTSVS DSTSASTSLS GSTSTSVSDS TSVSTSLSAS TSTSESDSTS
     TSTSLSGSTS TSVSDSISGS TSLSGSTSTS VSDSTSTSTS DSASTSTSVS DSTSASTSLS
     ASTSTSVSDS TSASTSLSAS TSTSVSDSTS ASTSLSASTS TSVSDSTSAS TSLSASTSTS
     VSDSTSTSTS LSASTSTSVS DSTSGSTSLS ASTSTSVSDS TSTSTSLSAS TSTSVSDSTS
     MSTSLSGSTS TSVSDSTSAS TSLSGSTSTS VSDSTSTSTS LSASTSTSVS DSTSTSTSLS
     GSTSTSVSDS TSASTSSSES TSTSVSDSTS ASVSTSISTS ISMSESSSTS ASTSDSTSTS
     ASTSESRSAS HSMSGTDSNN TSSSDSKSHS ISNSDSNTTS DSASASTSIS DSSSTSTSDS
     NASHSFSTSH SVSESNSMST SHSQFDSIST SESMSGTDST SLSTSLSHSA STSNSTSMTT
     SESQSNNDSQ MHSNSLHHDA KDELPDTGDS DSNSTGLVSA VAAMLAGLGL FGKSRKNKKD
     KKNKGSEQ