SRA_ECOLI
ID SRA_ECOLI Reviewed; 45 AA.
AC P68191; P28690; Q2MBA4;
DT 25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Stationary-phase-induced ribosome-associated protein;
DE Short=Protein D;
DE Short=SRA;
DE AltName: Full=30S ribosomal protein S22;
GN Name=sra; Synonyms=rpsV; OrderedLocusNames=b1480, JW5962;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-37, NOT A
RP RIBOSOMAL PROTEIN, SUBUNIT, AND PROTEIN COPY NUMBER.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=11292794; DOI=10.1128/jb.183.9.2765-2773.2001;
RA Izutsu K., Wada C., Komine Y., Sako T., Ueguchi C., Nakura S., Wada A.;
RT "Escherichia coli ribosome-associated protein SRA, whose copy number
RT increases during stationary phase.";
RL J. Bacteriol. 183:2765-2773(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2199308; DOI=10.1093/genetics/125.2.261;
RA Mahajan S.K., Chu C.C., Willis D.K., Templin A., Clark A.J.;
RT "Physical analysis of spontaneous and mutagen-induced mutants of
RT Escherichia coli K-12 expressing DNA exonuclease VIII activity.";
RL Genetics 125:261-273(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP IDENTIFICATION.
RX PubMed=3553168; DOI=10.1093/oxfordjournals.jbchem.a121866;
RA Wada A.;
RT "Analysis of Escherichia coli ribosomal proteins by an improved two
RT dimensional gel electrophoresis. I. Detection of four new proteins.";
RL J. Biochem. 100:1583-1594(1986).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=17277072; DOI=10.1128/jb.01713-06;
RA Bubunenko M., Baker T., Court D.L.;
RT "Essentiality of ribosomal and transcription antitermination proteins
RT analyzed by systematic gene replacement in Escherichia coli.";
RL J. Bacteriol. 189:2844-2853(2007).
RN [7]
RP MASS SPECTROMETRY.
RC STRAIN=K12 / ATCC 25404 / DSM 5698 / NCIMB 11290;
RX PubMed=10094780; DOI=10.1006/abio.1998.3077;
RA Arnold R.J., Reilly J.P.;
RT "Observation of Escherichia coli ribosomal proteins and their
RT posttranslational modifications by mass spectrometry.";
RL Anal. Biochem. 269:105-112(1999).
RN [8]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / BW25993;
RX PubMed=21903814; DOI=10.1126/science.1204697;
RA Wang W., Li G.W., Chen C., Xie X.S., Zhuang X.;
RT "Chromosome organization by a nucleoid-associated protein in live
RT bacteria.";
RL Science 333:1445-1449(2011).
CC -!- FUNCTION: Although this protein associates with the 30S subunit of the
CC ribosome it is not considered to be a bona fide ribosomal protein.
CC {ECO:0000269|PubMed:11292794, ECO:0000269|PubMed:17277072}.
CC -!- SUBUNIT: Associates exclusively with the 30S subunit; there is 0.1 copy
CC per ribosome in the exponential phase and 0.4 copies per ribosome in
CC the stationary phase. This is also true for several other K12
CC derivatives and strain B/R. {ECO:0000269|PubMed:11292794}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21903814}.
CC Note=Enriched in the periphery of the cell, excluded from the nucleoid
CC (PubMed:21903814). {ECO:0000269|PubMed:21903814}.
CC -!- INDUCTION: Induced upon entry into stationary phase, this is controlled
CC at least in part by the RpoS sigma factor.
CC -!- MASS SPECTROMETRY: Mass=5095.9; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10094780};
CC -!- DISRUPTION PHENOTYPE: Not essential, a quadruple raiA-hpf-rmf-sra
CC knockout strain is significantly outcompeted by wild-type after 4 days
CC growth (PubMed:17277072). {ECO:0000269|PubMed:17277072}.
CC -!- SIMILARITY: Belongs to the SRA family. {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be a ribosomal protein.
CC {ECO:0000305|PubMed:3553168}.
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DR EMBL; D13179; BAA02474.1; -; Genomic_DNA.
DR EMBL; X55956; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U00096; AAC74553.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76452.1; -; Genomic_DNA.
DR PIR; C64901; C64901.
DR RefSeq; NP_415997.1; NC_000913.3.
DR RefSeq; WP_000841554.1; NZ_STEB01000054.1.
DR AlphaFoldDB; P68191; -.
DR BioGRID; 4260209; 7.
DR DIP; DIP-48175N; -.
DR IntAct; P68191; 2.
DR STRING; 511145.b1480; -.
DR jPOST; P68191; -.
DR PaxDb; P68191; -.
DR PRIDE; P68191; -.
DR EnsemblBacteria; AAC74553; AAC74553; b1480.
DR EnsemblBacteria; BAE76452; BAE76452; BAE76452.
DR GeneID; 67417271; -.
DR GeneID; 946030; -.
DR KEGG; ecj:JW5962; -.
DR KEGG; eco:b1480; -.
DR PATRIC; fig|1411691.4.peg.787; -.
DR EchoBASE; EB1471; -.
DR eggNOG; ENOG5033FPW; Bacteria.
DR HOGENOM; CLU_210948_0_0_6; -.
DR PhylomeDB; P68191; -.
DR BioCyc; EcoCyc:EG11508-MON; -.
DR BioCyc; MetaCyc:EG11508-MON; -.
DR PRO; PR:P68191; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:EcoCyc.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR InterPro; IPR012607; Ribosomal_S22.
DR Pfam; PF08136; Ribosomal_S22; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; Reference proteome.
FT CHAIN 1..45
FT /note="Stationary-phase-induced ribosome-associated
FT protein"
FT /id="PRO_0000208690"
FT REGION 21..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 45 AA; 5096 MW; 81DB6E2D2E222C2F CRC64;
MKSNRQARHI LGLDHKISNQ RKIVTEGDKS SVVNNPTGRK RPAEK
