ID   SRB8_ASHGO              Reviewed;        1358 AA.
AC   Q751D2;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE   AltName: Full=Mediator complex subunit 12;
GN   Name=SRB8; Synonyms=MED12; OrderedLocusNames=AGL226C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 94 AND 100.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Component of the SRB8-11 complex. The SRB8-11 complex is a
CC       regulatory module of the Mediator complex which is itself involved in
CC       regulation of basal and activated RNA polymerase II-dependent
CC       transcription. The SRB8-11 complex may be involved in the
CC       transcriptional repression of a subset of genes regulated by Mediator.
CC       It may inhibit the association of the Mediator complex with RNA
CC       polymerase II to form the holoenzyme complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the SRB8-11 complex, which itself associates with
CC       the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC       {ECO:0000305}.
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DR   EMBL; AE016820; AAS54265.2; -; Genomic_DNA.
DR   RefSeq; NP_986441.2; NM_211503.2.
DR   AlphaFoldDB; Q751D2; -.
DR   SMR; Q751D2; -.
DR   STRING; 33169.AAS54265; -.
DR   PRIDE; Q751D2; -.
DR   EnsemblFungi; AAS54265; AAS54265; AGOS_AGL226C.
DR   GeneID; 4622734; -.
DR   KEGG; ago:AGOS_AGL226C; -.
DR   eggNOG; KOG4522; Eukaryota.
DR   HOGENOM; CLU_256280_0_0_1; -.
DR   InParanoid; Q751D2; -.
DR   OMA; EFIVYHQ; -.
DR   Proteomes; UP000000591; Chromosome VII.
DR   GO; GO:1990508; C:CKM complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0003713; F:transcription coactivator activity; IEA:EnsemblFungi.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0000411; P:positive regulation of transcription by galactose; IEA:EnsemblFungi.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:EnsemblFungi.
DR   InterPro; IPR019035; Mediator_Med12.
DR   Pfam; PF09497; Med12; 1.
DR   SMART; SM01281; Med12; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..1358
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   12"
FT                   /id="PRO_0000312964"
FT   COILED          629..654
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1358 AA;  158482 MW;  F5CF03A9EF60A3E8 CRC64;
     MSSSRYILIP PEDLHPLTSN TGNEQNIYPD FDPWAHTEIE DKILLSFVAK GHYTSAKVNF
     ESISARSSLQ ESLPKVAGML AEQFSKVVHL REQTINKVSG ESEESIRGKA KFTDLAGPGF
     SLPNRVTLTD QRRTQWLQEL SSPNVSLSKL AKSIPHGFKR KQILEQCYMR QLPLQRAIWL
     IKSSYSIEWK SLTSKLKPGQ TNEGVVSQLY NLWTNSMVSI MERLLFEMPK YYNDTAQAKI
     WKPRVSYYIN LLGNCYNMGL MDRGVFNHWL VEFVEKVENF EFLPLSLHLL NIFWSGICPP
     THGIEAPDNS FLISKITVVL LHKHHMILQN KSMINDENYI INDLQRNARL KESLLLRLKA
     FILDIFHHQS LEAFIMPNQN WDLYKNCLYE IVTMDKTSNE QAMVIKRKLE LITYRNDSLQ
     FNLLKQYKMK SSSLYESDGV EIDNLCDMQS ILNVPFLDPE MTKILDNATP GYDWTLFVQQ
     RFTCIEKVAQ MIMWATNPSR KARYDGAHLV AKLLLLKTTS QENLPDYNME DMIWKLVFHF
     SKLSERELSN IVELPSLYEL LNIFIGYGII KVPTYIRKLI SSGIMYISDS KDKFFHCELL
     INLKISPLMK NQYNMMLKNI MEYDATYYVR YNYEQLQIQL NTAKEQMLQE QFEHISCLPI
     SVKIWVSEWY LSYVCSPVDN VLKPVDKGTV IKNFTIFGLY LKEIFHFYKW VEFIVYHQLL
     ADINALSALI DVLLYYENLF PLLINDQILF MKTLIHIYSK GLKNKDNLSY NITEFNPFWK
     FFMKHFPYLV EIDSDLQLQL GEVYESEKIR IEKLTKSSVD VITLYCNINQ VNEESFKFGT
     HNFPGIFQQN LKILLKTESA DACERARKGL SLLMLANLGD YTKFMSIFLK RKDYTIEQLV
     KLISVKLLTL DQIQKIIGDD ILREILSRNN YGEGLWYELH KRNFIKKNFK IVLSMYRNST
     SLDDRKLFLD LLVFYGPNSR LQEQVTTIIC NCLRESNEDY SLILSLLRYG TKNIDQGTQE
     TINIAKLYEN LNFTNLWIFQ AFTNYYTEAL FNGATSDGQT ITNFVFELID LTRYNVLCSH
     LFDRVANFDV LEKLLEVFEA DFFKKCLSED PDDVHFLAVM IETIMNLSRK MNQSSAVISM
     NNESFRLLQA TMLKFTSMNK TALCNSEMKL SIYLKIFIVH QKFIFQKVCD SLRQDRYDAE
     ADLVKSLRLL FENTGFKLKL RLLLYDILSS LKSFVIYECT KKSESKRETR KLQISEALQN
     LPPFHISSFL DDMSISAHGD FDFLGLTEQP TPAEPEEGSQ FFLYNKRTRE YECGLNIEPF
     QLLPNHQSRE PGSSCHFFND TALSLSLFDA RFDKKNPT