SRB8_ASPOR
ID SRB8_ASPOR Reviewed; 1594 AA.
AC Q2U513;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 25-MAY-2022, entry version 65.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE AltName: Full=Mediator complex subunit 12;
GN Name=srb8; Synonyms=med12; ORFNames=AO090020000119;
OS Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=510516;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 42149 / RIB 40;
RX PubMed=16372010; DOI=10.1038/nature04300;
RA Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA Kikuchi H.;
RT "Genome sequencing and analysis of Aspergillus oryzae.";
RL Nature 438:1157-1161(2005).
CC -!- FUNCTION: Component of the srb8-11 complex. The srb8-11 complex is a
CC regulatory module of the Mediator complex which is itself involved in
CC regulation of basal and activated RNA polymerase II-dependent
CC transcription. The srb8-11 complex may be involved in the
CC transcriptional repression of a subset of genes regulated by Mediator.
CC It may inhibit the association of the Mediator complex with RNA
CC polymerase II to form the holoenzyme complex (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the srb8-11 complex, which itself associates with
CC the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE63352.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP007167; BAE63352.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; Q2U513; -.
DR SMR; Q2U513; -.
DR STRING; 510516.Q2U513; -.
DR PRIDE; Q2U513; -.
DR Proteomes; UP000006564; Chromosome 6.
DR GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR InterPro; IPR019035; Mediator_Med12.
DR Pfam; PF09497; Med12; 1.
DR SMART; SM01281; Med12; 1.
PE 3: Inferred from homology;
KW Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..1594
FT /note="Mediator of RNA polymerase II transcription subunit
FT 12"
FT /id="PRO_0000312967"
FT REGION 1..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1499..1532
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..52
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 101..116
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1594 AA; 175558 MW; 8EE6ABE361B3A3AB CRC64;
MIPHSSAGVQ SWGHPLRAVN NGSGHVDASQ AVGPPDPQFE KLPTPVPQPQ PRQPAVIDLT
TSGGDAQELE PPPKRLRLDL PAAPSARDAS PAPGSGGELR STPGTGGSKP PSLSWRNRPV
WSFQAMLSEV PGSNVMNEED ATAVAQGGKP ASPPSLPVLP WKYIPESLGS NPTTSRASSP
VKEVQTIPYR IETPSVAPVL KGEKVADFSP WIGNHPEDVL NEQTAKQGHY DRTQVSQNES
NTARPSLYAQ LKHRSGLQML SSVFAAALEK RQNHSLVTAP STFKPPPRVT LTDNKREAWL
RDLANPSVPL RKLSRTIPHG IRGKALLDQC LNKGIPVNRA VWLAKCVGAN EIRAFKRKGT
SGTLALGLEA KWVRDWTASV QQFLEGVLGA CGSAQWKMKM TYAVSLTARL FFERLLDHDQ
YLGWFLSSLE AAPVNIVPVW LLMLGIYWDN IMRYRKRGRR LAELLLVKLR QVTQPDKGGP
LQPLVDRLSL YVRRLVLEHT SSVVLPGSWE NHKELISSCL NLKDNVHKTI YQNLSERNSR
LQLPKNHQDT AERSPQQRVI QLFDSIRSAH DISSASTACL KTIEDKAILI SKLLEWTATP
FRYGLCRVYT GVRLLRKWKM SGIDVDSYIL SFLADVRVTS ALNMENIYHI ISELVRSQTF
SVGRYLQWLM AKGVTNTPQP VPSDLCLLKQ LPANRLPEHV RNLRNTLLYR AGIPVMEEDS
AIAELKISIA QRLPNIFGAE MDSAMPTESS QPHPTWAVKS EIGQWIRHGI AGHCRDSPRY
VYLLSRSTAP ANNYRKLSGV SVAVDPGASA LTPDEFYSVR EILETFGDLS MLADILKQAT
RCDDDVVLAS VADTVNCHFD CFCVIGATAD LFRGLVESYA RLKRLGNASL DLLFSLIELG
LRIPSEFNTV ALLRQDLTRL ENKSALAAPS PLSDSIPLAL SDVDPSFQEK LNQLLSSGGG
MDESTMDTVF YSLMHILENS GSPAKLSANE TARYLAYLRP FQPKHFDTML IRWICGLLKS
STPSMSRILP PLIGVGCVTI HAFVFLVKKL LQSEKVAAVI PNLAGLRVDL LQLLVPLVSG
KSKYADLVTY RFHVAQQEFF MKHPQETLDI ICDAVALVNS ETGSNPGQPD IAGCATELLD
ILLTQNPEVT VQYCLQGFIG KHSTSTTVLE RALDNLLGFD SLAGPPTMSE AEKVVRMTDD
FSLPFCQLKL QMLFNAESGR NVGNGIVDVM FKAAVEDTRS KGSNWVGFVG LMSQDIIRQV
WKHHYHYKNL EAHGHPGTAK SLETAKLYLT IIEKLAYSVP EAGVQSVAPI LVEKMDLLLH
RLVIMQTNFN NVTMNRHGAA TTQILQSRSN FERSLAFWFS AFLRMIVIHR SAFTMPSPAP
RANGLQEQSR LLISILCISL ARLPDSVIRL FPAADYFPHP IPSQGYRPCP GILLQTHALD
VAASLIDTFP DEARQQCARF LKEKCPPFLQ YQNDSRFIYL LGPMSDAAAL NSLQAASLPS
PAAGGSTPTP TPSSALPGAP SNPQPTAMTP AVTSASLSEG INCVASHLRL QYRGRAMGPY
PVRPWELLED AAPIVGVNDT AVNLKYFDAR RVRA
